A4.05 SMOC2, a secreted calcium-binding protein from cartilage extracellular matrix is an inhibitor of cartilage and bone formation

2016 ◽  
Vol 75 (Suppl 1) ◽  
pp. A38.3-A39
Author(s):  
T Peeters ◽  
F Cailotto ◽  
RJ Lories
Keyword(s):  
Extracellular Matrix ◽  
Bone Formation ◽  
Calcium Binding ◽  
Binding Protein ◽  
Calcium Binding Protein ◽  
Cartilage Extracellular Matrix

scholarly journals Association of an extracellular protein (chondrocalcin) with the calcification of cartilage in endochondral bone formation.

1984 ◽  
Vol 98 (1) ◽  
pp. 54-65 ◽  
Author(s):  
A R Poole ◽  
I Pidoux ◽  
A Reiner ◽  
H Choi ◽  
L C Rosenberg
Keyword(s):  
Extracellular Matrix ◽  
Bone Formation ◽  
Growth Plate ◽  
Calcium Binding ◽  
Calcium Binding Protein ◽  
Cartilage Matrix ◽  
Hypertrophic Chondrocytes ◽  
Endochondral Bone Formation ◽  
Endochondral Bone ◽  
Nucleation Sites

We examined bovine fetal epiphyseal and growth plate cartilages by immunofluorescence microscopy and immunoelectron microscopy using monospecific antibodies to a newly discovered cartilage-matrix calcium-binding protein that we now call chondrocalcin. Chondrocalcin was evenly distributed at relatively low concentration in resting fetal epiphyseal cartilage. In growth plate cartilage, it was absent from the extracellular matrix in the zone of proliferating chondrocytes but was present in intracellular vacuoles in proliferating, maturing and upper hypertrophic chondrocytes. The protein then disappeared from the lower hypertrophic chondrocytes and appeared in the adjoining extracellular matrix, where it was selectively concentrated in the longitudinal septa in precisely the same location where amorphous mineral was deposited in large amounts as demonstrated by von Kossa staining and electron microscopy. Mineral then spread out from these "nucleation sites" to occupy much of the surrounding matrix. Matrix vesicles were identified in this calcifying matrix but they bore no observable morphological relationship to these major sites of calcification where chondrocalcin was concentrated. Since chondrocalcin is a calcium-binding protein and has a strong affinity for hydroxyapatite, these observations suggest that chondrocalcin may play a fundamental role in the creation of nucleation sites for the calcification of cartilage matrix in endochondral bone formation.

Calcium-binding protein 1 of Entamoeba histolytica transiently associates with phagocytic cups in a calcium-independent manner

2008 ◽  
Vol 10 (6) ◽  
pp. 1373-1389 ◽  
Author(s):  
Ruchi Jain ◽  
Julien Santi-Rocca ◽  
Narendra Padhan ◽  
Sudha Bhattacharya ◽  
Nancy Guillen ◽  
...  
Keyword(s):  
Entamoeba Histolytica ◽  
Calcium Binding ◽  
Binding Protein ◽  
Calcium Binding Protein ◽  
Independent Manner ◽  
Phagocytic Cups

scholarly journals Regulation of adenylate cyclase from glial tumor cells by calcium and a calcium-binding protein.

1976 ◽  
Vol 251 (15) ◽  
pp. 4744-4750 ◽  
Author(s):  
M A Brostrom ◽  
C O Brostrom ◽  
B M Breckenridge ◽  
D J Wolff
Keyword(s):  
Adenylate Cyclase ◽  
Tumor Cells ◽  
Calcium Binding ◽  
Binding Protein ◽  
Calcium Binding Protein ◽  
Glial Tumor
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