Effects of miRNA-136 overexpression on S100 calcium binding protein A6 contents, proliferation and apoptosis in HCT116 cells

Recurrent implantation failure (RIF) and recurrent pregnancy loss (RPL) are distressing conditions without effective treatments. The luminal epithelium (LE) is integral in determining receptivity of the endometrium, whereas functionalis glands and stroma aid in nurturing early embryo development. Calcium signalling pathways are known to be of vital importance to embryo implantation and pregnancy establishment, and anterior gradient protein 3 (AGR3) and S100 calcium-binding protein P (S100P) are involved with these pathways. We initially examined 20 full-thickness endometrial biopsies from premenopausal women across the menstrual cycle to characterize levels of AGR3 protein in each endometrial sub-region at the cellular level. A further 53 endometrial pipelle biopsies collected in the window of implantation were subsequently assessed to determine differential endometrial AGR3 and S100P levels relevant to RIF (n = 13) and RPL (n = 10) in comparison with parous women (n = 30) using immunohistochemistry. Significantly higher AGR3 and S100P immunostaining was observed in ciliated cells of the LE of women with recurrent reproductive failure compared with parous women, suggesting aberrant subcellular location-associated pathophysiology for these conditions. The nuclear localisation of S100P may allow transcriptional regulatory function, which is necessary for implantation of a viable pregnancy. Further work is thus warranted to assess their utility as diagnostic/therapeutic targets.

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S100A4 (S100 Calcium Binding Protein A4)

Atlas of Genetics and Cytogenetics in Oncology and Haematology ◽

10.4267/2042/53767 ◽

2014 ◽

Author(s):

GV Sherbet

Keyword(s):

Calcium Binding ◽

Binding Protein ◽

Calcium Binding Protein ◽

S100 Calcium Binding Protein

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S100 Calcium-Binding Protein

Encyclopedia of Signaling Molecules ◽

10.1007/978-3-319-67199-4_103387 ◽

2018 ◽

pp. 4793-4793

Keyword(s):

Calcium Binding ◽

Binding Protein ◽

Calcium Binding Protein ◽

S100 Calcium Binding Protein

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Involvement of Regucalcin in Protein Synthesis and Proteolysis: Regulation of the Enzyme Activity in the Cytoplasm and Nucleus

Integrative Biomedical Sciences ◽

10.18314/gjbs.v1i2.52 ◽

2015 ◽

Vol 1 (2) ◽

Author(s):

Masayoshi Yamaguchi

Keyword(s):

Protein Synthesis ◽

Calcium Binding ◽

Binding Protein ◽

Suppressive Effect ◽

Trna Synthetase ◽

Calcium Binding Protein ◽

Proliferation And Apoptosis ◽

Regucalcin Gene ◽

Proteolysis Regulation ◽

Nuclear Deoxyribonucleic Acid

Regucalcin was discovered in 1978 as a novel calcium-binding protein. The name, regucalcin, was proposed for this calcium-binding protein, which regulates various Ca2+- or Ca2+/calmodulin-dependent enzyme activations. The regucalcin gene (gene symbol;rgn) is localized on the X chromosome. Regucalcin has been demonstrated to play a multifunctional role in the regulation of intracellular calcium homeostasis, signal transduction, gene expression, cell proliferation and apoptosis in various types of cells and tissues. The cytoplasmic regucalcin translocases to the nucleus and suppresses nuclear deoxyribonucleic acid (DNA) and ribonucleic acid (RNA) synthesis. Moreover, regucalcin has been shown to reveal a suppressive effect on protein synthesis and a stimulatory effect on protein degradation. Regucalcin has been found to inhibit aminoacyl-tRNA synthetase and activate thiol protease. Â Regucalcin may play a suppressive role in the regulation of protein turnover in cells.

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