AB0017 CONSTANT GENETIC MARKER IL1B T-31С IS ASSOCIATED WITH ANAMNESIS OF BIOLOGICAL DRUGS TREATMENT IN RHEUMATOID ARTHRITIS

Background:Rheumatoid arthritis (RA) is chronic progressive joint disease with erosions formation. Timely and effectiveness treatment is important due to quickly structural damage and progressive losing of active motion. Synthetic DMARDs didn’t have a sufficient effect. Using biological drugs seemed like a panacea, but according to investigations at least 30-40% RA-patients lost treatment efficiency. Biological drugs act through immune cascade, that’s why mutation in regulatory region of cytokine genes may partly determine treatment failure.Objectives:The objective of our study was to analyze the frequency ofIL1 T-31Csingle nucleotide polymorphism in patient with rheumatoid arthritis and its association with biological drugs prescribing.Methods:One hundred two Caucasian RA-patients (age – 56 yrs [45; 61]; DAS28 4.7 [3.8; 5.9]) were enrolled in our study. All of them had American College of Rheumatology (ACR)-defined RA (1987 classification criteria) and gave written inform consent. Single nucleotide polymorphismsIL1B T-31C(rs1143627),IL4 C-590T(rs2243250), IL10 C-592A (rs1800872),IL10 A-1082G(rs1800896) were determined by restriction fragment length polymorphism. Descriptive statistics, Chi-squared test were used for data analysis. Results are presented as median and 25th/75th percentiles (Me [25th percentile; 75th percentile]).Results:The most of SNPs analyzed had corresponded to the Hardy Weinberg equilibrium (HWE). The only exception was IL1B T-31C – the frequencies were differed statistically significant from HWE (p=0,03). Forty seven (46.1%) patients were treatment with biological drugs. Homozygotes IL1b -31CС were founded more frequently beside patients with biological treatment compare with other group (13 from 47 (27,7%) vs. 6 from 52 (11.5%), p=0,042). Other SNPs didn’t demonstrate any associations.Conclusion:Single nucleotide polymorphismIL1B T-31C(rs1143627) may be used for prognosis of basic anti-inflammatory therapy inefficiency and the needing for prescribing biological therapy.Disclosure of Interests:None declared

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Association of single nucleotide polymorphism at position −308 of the tumor necrosis factor-alpha gene with ankylosing spondylitis and rheumatoid arthritis

Biotechnology & Biotechnological Equipment ◽

10.1080/13102818.2014.972147 ◽

2014 ◽

Vol 28 (6) ◽

pp. 1108-1114 ◽

Cited By ~ 10

Author(s):

Irena Manolova ◽

Mariana Ivanova ◽

Rumen Stoilov ◽

Rasho Rashkov ◽

Spaska Stanilova

Keyword(s):

Rheumatoid Arthritis ◽

Single Nucleotide Polymorphism ◽

Ankylosing Spondylitis ◽

Tumor Necrosis ◽

Nucleotide Polymorphism ◽

Necrosis Factor Alpha ◽

Single Nucleotide ◽

Factor Alpha ◽

Alpha Gene ◽

Necrosis Factor

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Genetic association of CCR5 promoter single nucleotide polymorphism in seronegative and seropositive rheumatoid arthritis

European Cytokine Network ◽

10.1684/ecn.2012.0305 ◽

2012 ◽

Vol 23 (2) ◽

pp. 25-28

Author(s):

Guadalupe Lima ◽

Janette Furuzawa-Carballeda ◽

Dolores Ramos-Bello ◽

Juan Jakez-Ocampo ◽

Virginia Pascual-Ramos ◽

...

Keyword(s):

Rheumatoid Arthritis ◽

Single Nucleotide Polymorphism ◽

Genetic Association ◽

Nucleotide Polymorphism ◽

Single Nucleotide ◽

Seropositive Rheumatoid Arthritis

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A variant of theIL4I50V single‐nucleotide polymorphism is associated with erosive joint disease in psoriatic arthritis

Arthritis & Rheumatism ◽

10.1002/art.23558 ◽

2008 ◽

Vol 58 (7) ◽

pp. 2207-2208 ◽

Cited By ~ 13

Author(s):

Proton Rahman ◽

Tara Snelgrove ◽

Lynette Peddle ◽

Fiotos Siannis ◽

Vernon Farewell ◽

...

Keyword(s):

Single Nucleotide Polymorphism ◽

Psoriatic Arthritis ◽

Joint Disease ◽

Nucleotide Polymorphism ◽

Single Nucleotide

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A single nucleotide polymorphism of IL6-receptor is associated with response to tocilizumab in rheumatoid arthritis patients

The Pharmacogenomics Journal ◽

10.1038/s41397-019-0072-6 ◽

2019 ◽

Vol 19 (4) ◽

pp. 368-374 ◽

Cited By ~ 5

Author(s):

Cécile Luxembourger ◽

Adeline Ruyssen-Witrand ◽

Chayma Ladhari ◽

Cécile Rittore ◽

Yannick Degboe ◽

...

Keyword(s):

Rheumatoid Arthritis ◽

Single Nucleotide Polymorphism ◽

Nucleotide Polymorphism ◽

Single Nucleotide

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Reduced Anti-Histone Antibodies and Increased Risk of Rheumatoid Arthritis Associated with a Single Nucleotide Polymorphism in PADI4 in North Americans

International Journal of Molecular Sciences ◽

10.3390/ijms20123093 ◽

2019 ◽

Vol 20 (12) ◽

pp. 3093 ◽

Cited By ~ 2

Author(s):

Aisha M. Mergaert ◽

Mandar Bawadekar ◽

Thai Q. Nguyen ◽

Laura Massarenti ◽

Caitlyn L. Holmes ◽

...

Keyword(s):

Rheumatoid Arthritis ◽

Single Nucleotide Polymorphism ◽

Enzyme Linked Immunosorbent Assay ◽

Human Rheumatoid Arthritis ◽

Nucleotide Polymorphism ◽

Single Nucleotide ◽

Rheumatoid Arthritis Diagnosis ◽

North Americans ◽

Increased Risk ◽

Citrullinated Proteins

Autoantibodies against citrullinated proteins are a hallmark of rheumatoid arthritis, a destructive inflammatory arthritis. Peptidylarginine deiminase 4 (PAD4) has been hypothesized to contribute to rheumatoid arthritis by citrullinating histones to induce neutrophil extracellular traps (NETs), which display citrullinated proteins that are targeted by autoantibodies to drive inflammation and arthritis. Consistent with this theory, PAD4-deficient mice have reduced NETs, autoantibodies, and arthritis. However, PAD4′s role in human rheumatoid arthritis is less clear. Here, we determine if single nucleotide polymorphism rs2240335 in PADI4, whose G allele is associated with reduced PAD4 in neutrophils, correlates with NETs, anti-histone antibodies, and rheumatoid arthritis susceptibility in North Americans. Control and rheumatoid arthritis subjects, divided into anti-cyclic citrullinated peptide (CCP) antibody positive and negative groups, were genotyped at rs2240335. In homozygotes, in vitro NETosis was quantified in immunofluorescent images and circulating NET and anti-histone antibody levels by enzyme linked immunosorbent assay (ELISA). Results were compared by t-test and correlation of rheumatoid arthritis diagnosis with rs2240335 by Armitage trend test. NET levels did not significantly correlate with genotype. G allele homozygotes in the CCP− rheumatoid arthritis group had reduced anti-native and anti-citrullinated histone antibodies. However, the G allele conferred increased risk for rheumatoid arthritis diagnosis, suggesting a complex role for PAD4 in human rheumatoid arthritis.

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The His155Tyr (489C>T) single nucleotide polymorphism of P2RX7 gene confers an enhanced function of P2X7 receptor in immune cells from patients with rheumatoid arthritis

Cellular Immunology ◽

10.1016/j.cellimm.2012.05.005 ◽

2012 ◽

Vol 276 (1-2) ◽

pp. 168-175 ◽

Cited By ~ 28

Author(s):

Liliana Portales-Cervantes ◽

Perla Niño-Moreno ◽

Mariana Salgado-Bustamante ◽

Mariana H. García-Hernández ◽

Lourdes Baranda-Candido ◽

...

Keyword(s):

Rheumatoid Arthritis ◽

Single Nucleotide Polymorphism ◽

Immune Cells ◽

P2x7 Receptor ◽

Nucleotide Polymorphism ◽

Single Nucleotide

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Disease Phenotypes and Gender Association of FCRL3 Single-Nucleotide Polymorphism −169T/C in Taiwanese Patients with Systemic Lupus Erythematosus and Rheumatoid Arthritis

The Journal of Rheumatology ◽

10.3899/jrheum.100437 ◽

2010 ◽

Vol 38 (2) ◽

pp. 264-270 ◽

Cited By ~ 21

Author(s):

JI-YIH CHEN ◽

CHIN-MAN WANG ◽

YEONG-JIAN JAN WU ◽

SHIN-NING KUO ◽

CHIUNG-FANG SHIU ◽

...

Keyword(s):

Rheumatoid Arthritis ◽

Systemic Lupus Erythematosus ◽

Single Nucleotide Polymorphism ◽

Lupus Erythematosus ◽

Nucleotide Polymorphism ◽

Systemic Lupus ◽

Single Nucleotide ◽

Disease Phenotypes ◽

And Gender ◽

Non Destructive

Objective.To investigate the association of the functional FCRL3 single-nucleotide polymorphism (SNP) −169T/C with disease phenotypes and susceptibility to systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA) in Taiwanese.Methods.FCRL3 SNP −169T/C was genotyped in 573 patients with SLE, 670 patients with RA, and 758 controls. Genotype distributions and allele frequencies were compared among the 3 groups as aggregates or as stratified by clinical characteristics, autoantibody profile, and sex within patient groups.Results.Overall, FCRL3 SNP −169T/C was not associated with susceptibility to either SLE or RA. However, −169CC genotype was significantly reduced in leukopenia-positive SLE patients as compared to the leukopenia-negative SLE patients (CC vs CT+TT, p = 6 × 10−4, OR 0.444, 95% CI 0.279–0.708) and controls (p = 6.1 × 10−3, OR 0.583, 95% CI 0.396–0.857). On the other hand, −169TT genotypes were significantly more numerous in RA patients with non-destructive disease as compared with patients with destructive disease (CC+CT vs TT: p = 0.007, OR 1.672, 95% CI 1.149–2.432). The −169T allele frequency was also significantly increased in non-destructive RA compared with patients with destructive disease (C vs T: p = 0.010, OR 1.423, 95% CI 1.089–1.859). FCRL3 SNP −169TT homozygous donors were significantly more numerous among female cyclic citrullinated peptide (CCP)-negative RA patients versus female CCP-positive RA patients (CC+CT vs TT: p = 0.019, OR 1.64, 95% CI 1.085–2.479).Conclusion.The functional FCRL3 SNP −169T/C appears to play important roles in the development of certain phenotypes such as SLE leukopenia and RA disease severity in Taiwanese patients with SLE and RA.

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