An introduction of an easy-operating and economical technique for tissue microarray preparation

2019 ◽  
Vol 73 (7) ◽  
pp. 403-407
Author(s):  
Yi-Jing Chen ◽  
Chun-Mei Yang ◽  
Jiang-Sheng Huang ◽  
Ping Wang ◽  
Yan-Hua Lv ◽  
...  

AimTissue microarray (TMA) is a powerful and effective tool for in situ tissue analysis. However, manual TMA construction methods showed varied qualities. This study aimed to raise a standardised TMA preparation technique that can be easily operated and is economical.MethodsA sampling needle was used to punch the tissue rods from the donor block and holes in the recipient block. To indicate the dots’ positions and ensure vertical punching, a novel auxiliary device made using commercial three-dimensional printing technology was attached. The TMA block was made up of tissue rods and a recipient block.ResultsA 77-rod (7×11) TMA block was constructed. The rows and columns were fixed in straight lines. There was no specimen loss during the process of embedding.ConclusionsAn alternative method for the construction of TMA blocks that met the basic requirement of many laboratories and can be effortlessly performed was presented.

2019 ◽  
Vol 97 ◽  
pp. 06018 ◽  
Author(s):  
Anastasiia Sharanova ◽  
Maria Dmitrieva

Three-dimensional printing is an innovative digital technology and is one of the most significant achievements in the industrial and technological development of modernity. Digital 3D technologies have reached a colossal level of development over a period of about half a century since their invention. Using 3D printing in the construction of low-rise buildings, it becomes possible to implement almost any ideas and achieve the best architectural expressiveness of objects. The aim of this work is to create cement-based compounds that can be used in additive construction methods. A review of the scientific literature on this topic was carried out, the main components of the mixture were identified, and a series of field experiments were conducted. According to the results of the work, the optimal composition for 3D construction printing was determined.


2020 ◽  
Vol 4 (1) ◽  
Author(s):  
Kiril Stoyanov ◽  
◽  
◽  

Some of the most impressive architectural masterpieces of the past, such as the Cologne Cathedral or the Cathedral in Milan, have taken several centuries for their creation and have involved thousands of people. Today, a glossy office building in Dubai has been built or rather printed in just 17 days, with 29 workers involved. Three-dimensional printing has been identified as the Fourth Industrial Revolution and is increasingly replacing traditional construction methods. There is no doubt that 3D printing will affect the design and production of buildings. Does the fact that 3D printing can be used means that it should replace analog with digital construction?


2009 ◽  
Vol 00 (00) ◽  
pp. 090730035508060-7
Author(s):  
Deng-Guang Yu ◽  
Chris Branford-White ◽  
Yi-Cheng Yang ◽  
Li-Min Zhu ◽  
Edward William Welbeck ◽  
...  

2020 ◽  
Vol 13 (12) ◽  
pp. e239286
Author(s):  
Kumar Nilesh ◽  
Prashant Punde ◽  
Nitin Shivajirao Patil ◽  
Amol Gautam

Ossifying fibroma (OF) is a rare, benign, fibro-osseous lesion of the jawbone characterised by replacement of the normal bone with fibrous tissue. The fibrous tissue shows varying amount of calcified structures resembling bone and/or cementum. The central variant of OF is rare, and shows predilection for mandible among the jawbone. Although it is classified as fibro-osseous lesion, it clinically behaves as a benign tumour and can grow to large size, causing bony swelling and facial asymmetry. This paper reports a case of large central OF of mandible in a 40-year-old male patient. The lesion was treated by segmental resection of mandible. Reconstruction of the surgical defect was done using avascular fibula bone graft. Role of three-dimensional printing of jaw and its benefits in surgical planning and reconstruction are also highlighted.


Author(s):  
Leandro Ejnisman ◽  
Bruno Gobbato ◽  
Andre Ferrari de França Camargo ◽  
Eduardo Zancul

Author(s):  
Jorge Alfonso Tavares-Negrete ◽  
Alberto Emanuel Aceves-Colin ◽  
Delia Cristal Rivera-Flores ◽  
Gladys Guadalupe Díaz-Armas ◽  
Anne-Sophie Mertgen ◽  
...  

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