Bubbled Roller Compacted Concrete Dam: an idea of a hollow dam

Roller compacted concrete is widely known for its relatively low cost and short construction time. RCC gravity dams require high foundation (rock) bearing capacity. Research has been carried out which proposes to rationalise the amount of material in the dam by creating inner voids, in the shape of bubbles. As a key requirement, the introduced bubbles should not affect the dam stability and safety. The bubbles will reduce the self-weight of the dam and minimise the required rock bearing capacity. A system of pipes connecting the bubbles ensures drainage of the bubbles. The proposed dam would save about 12% of the required concrete volume. Different construction methods were studied and the best alternative is the use of precast hollow boxes. This decreases RCC placement in the dam by 32.5%. The objective is to speed the construction process and minimise the risk of the heat of hydration.

Comparison of double-stranded and single-stranded cfDNA library construction methods for targeted genome and methylation sequencing

Cell-free DNA (cfDNA) profiling by deep sequencing (i.e., by next generation sequencing (NGS)) has wide applications in cancer diagnosis, prognosis, and therapy response monitoring. One key step of cfDNA deep sequencing workflow is NGS library construction, whose efficiency significantly affects the utilization efficiency of cfDNA molecules, and eventually determines effective sequencing depth and sequencing accuracy. In this study, we compared two different types of cfDNA library construction methods, namely double-stranded library (dsLib, the conventional method which captures dsDNA molecules) and single-stranded library (ssLib) preparation, which captures ssDNA molecules, for the applications of mutation detection and methylation profiling, respectively. Our results suggest that the dsLib method was suitable for mutation detection while the ssLib method proved more efficient for methylation analysis. Our findings could help researchers choose the more appropriate library construction method for corresponding downstream applications of cfDNA sequencing.

Excavations at the Iron Age Village Site of Fibobe II, Central Zambia

The period from c. AD 900 to AD 1300 in southern Africa is characterized by transitions from small-scale Iron Age mixed economy communities to the beginnings of more intensive food production and eventually the emergence of complex polities. In Zambia, this coincides with the appearance of larger and more permanent agro-pastoralist villages that began participating in Indian Ocean trade networks. Unlike other parts of southern Africa where stone architecture became common, the predominance of wattle-and-daub type construction methods across Zambia have often impeded preservation of Iron Age activity areas. It has therefore been difficult to reconstruct how economic and land-use changes between the Early and Later Iron Ages impacted family and community relationships reflected in intra-site and intra-household spatial organization. Fibobe II, in the Mulungushi River Basin of Central Zambia, is a rare example of an Early-to-Mid Iron Age village site where these spatial patterns may be discernable due to preservation of activity spaces and vitrified remains of wattle-and-daub structures. This paper reports on new investigations following original testing of the site in 1979, confirming preservation of an Iron Age hut with distinct patterning of features, artifacts, and charcoal. These results reaffirm the unique nature of Fibobe II and indicate the potential for programs of household archaeology aimed at studying this important and understudied period in Zambian prehistory.

Hackflex: low-cost, high-throughput, Illumina Nextera Flex library construction

We developed a low-cost method for the production of Illumina-compatible sequencing libraries that allows up to 14 times more libraries for high-throughput Illumina sequencing to be generated for the same cost. We call this new method Hackflex. The quality of library preparation was tested by constructing libraries from Escherichia coli MG1655 genomic DNA using either Hackflex, standard Nextera Flex (recently renamed as Illumina DNA Prep) or a variation of standard Nextera Flex in which the bead-linked transposase is diluted prior to use. In order to test the library quality for genomes with a higher and a lower G+C content, library construction methods were also tested on Pseudomonas aeruginosa PAO1 and Staphylococcus aureus ATCC 25923, respectively. We demonstrated that Hackflex can produce high-quality libraries and yields a highly uniform coverage, equivalent to the standard Nextera Flex kit. We show that strongly size-selected libraries produce sufficient yield and complexity to support de novo microbial genome assembly, and that assemblies of the large-insert libraries can be much more contiguous than standard libraries without strong size selection. We introduce a new set of sample barcodes that are distinct from standard Illumina barcodes, enabling Hackflex samples to be multiplexed with samples barcoded using standard Illumina kits. Using Hackflex, we were able to achieve a per-sample reagent cost for library prep of A$7.22 (Australian dollars) (US $5.60; UK £3.87, £1=A$1.87), which is 9.87 times lower than the standard Nextera Flex protocol at advertised retail price. An additional simple modification and further simplification of the protocol by omitting the wash step enables a further price reduction to reach an overall 14-fold cost saving. This method will allow researchers to construct more libraries within a given budget, thereby yielding more data and facilitating research programmes where sequencing large numbers of libraries is beneficial.

A viewpoint on material and design considerations for oesophageal stents with extended lifetime

Oesophageal stents are meshed tubular implants designed to maintain patency of the oesophageal lumen and attenuate the symptoms of oesophageal cancer. Oesophageal cancers account for one in twenty cancer diagnoses and can lead to dysphasia, malnutrition and the diminishment of patient quality of life (QOL). Self-expanding oesophageal stents are the most common approach to attenuate these symptoms. Recent advances in oncological therapy have enabled patient survival beyond the lifetime of current devices. This introduces new complications for palliation, driving the need for innovation in stent design. This review identifies the factors responsible for stent failure. It explores the challenges of enhancing the longevity of stent therapies and outlines solutions to improving clinical outcomes. Discussions focus on the role of stent materials, construction methods, and coatings upon device performance. We found three key stent enhancement strategies currently used; material surface treatments, anti-migratory modifications, and biodegradable skeletons. Furthermore, radioactive and drug eluting stent designs were identified as emerging novel treatments. In conclusion, the review offers an overview of remaining key challenges in oesophageal stent design and potential solutions. It is clear that further research is needed to improve the clinical outcome of stents and patient QOL.

Chemical conversion based on crystal facet effect of transition metal oxide and construction methods for sharp-faced nanocrystals

The in-depth researches have found that the nanocrystal facet of transition metal oxide (TMO) greatly affects its heterogeneous catalytic performance, as well as the property of photocatalysis, gas sensing, electrochemical...

Vesicle-based artificial cells: Materials, construction methods and applications

The construction of artificial cells with specific cell-mimicking functions helps to explore complex biological processes and cell functions in natural cell systems, and provides insight into the origins of life....

Analysis of Construction Monitoring and Control of Tunnels Crossing Broken Faults and Water-rich Geology

In view of the poor geology such as tunnel engineering crossing faults or passing faults, the construction of surrounding rock and support is complicated. During construction, it is necessary to ensure the stability of the surrounding rock and supporting system, and ensure the timing of the secondary lining construction. For this reason, through the analysis and processing of monitoring data, the law of stratum change is mastered, and the supporting parameters and construction methods are adjusted and revised at the same time, so as to provide the best information for the tunnel surrounding rock and supporting lining construction.