scholarly journals Vitamin B12 and folate concentrations in serum and cerebrospinal fluid of neurological patients with special reference to multiple sclerosis and dementia.

1990 ◽  
Vol 53 (11) ◽  
pp. 951-954 ◽  
Author(s):  
T Q Nijst ◽  
R A Wevers ◽  
H C Schoonderwaldt ◽  
O R Hommes ◽  
A F de Haan
Keyword(s):  
Multiple Sclerosis ◽  
Cerebrospinal Fluid ◽  
Vitamin B12 ◽  
Special Reference ◽  
Neurological Patients

Detection of JC virus DNA in cerebrospinal fluid from multiple sclerosis patients

1998 ◽  
Vol 4 (2) ◽  
pp. 49-54 ◽  
Author(s):  
P Ferrante ◽  
E Omodeo-Zorini ◽  
R Caldarelli-Stefano ◽  
M Mediati ◽  
E Fainardi ◽  
...  
Keyword(s):  
Multiple Sclerosis ◽  
Cerebrospinal Fluid ◽  
Neurological Disorders ◽  
Healthy Subjects ◽  
Jc Virus ◽  
Control Groups ◽  
Peripheral Blood Mononuclear ◽  
Neurological Patients ◽  
Sequences Analysis ◽  
Multiple Sclerosis Patients

JC virus (JCV), the causative agent of progressive multifocal leukoencephalopathy (PML), has been proposed as a possible aetiopathogenic factor in multiple sclerosis (MS). We performed a study to search the LT region of JCV genome by nested PCR in cerebrospinal fluid (CSF), peripheral blood mononuclear cell (PBMC) and urine samples collected from 121 MS patients, 24 patients with other neurological disorders (OND), 30 non neurological patients (NND) and in PBMCs and urine of 40 healthy subjects. JCV DNA has been found in the CSF of 11 MS patients (9%) while all the CSFs from the 24 OND and the 30 NND cases were negative. No significant differences have been observed as regard to the frequency of JCV DNA detection in PBMCs and urine between the MS patients and the control groups. Nucleotide sequences analysis of seven JCV CSF isolates showed that five strains were identical the prototypal strain, while the other two had a base mutation (T→C) in 4286 nucleotide (nt). The finding of JCV DNA in the CSF of MS patients suggest that JCV could play a role in the triggering and/or in the maintenance of MS aetiopathogenic process, and therefore it should be taken in consideration when monitoring this disease.

Utility of isoelectric focusing of cerebrospinal fluid and serum on agarose evaluated for neurological patients.

1983 ◽  
Vol 29 (5) ◽  
pp. 810-815 ◽  
Author(s):  
H Link ◽  
V Kostulas
Keyword(s):  
Multiple Sclerosis ◽  
Cerebrospinal Fluid ◽  
Cerebral Infarction ◽  
Isoelectric Focusing ◽  
Neurological Diseases ◽  
Oligoclonal Bands ◽  
Good Reproducibility ◽  
Routine Examination ◽  
Neurological Patients ◽  
Agarose Electrophoresis

Abstract Agarose isoelectric focusing was used to demonstrate oligoclonal bands in cerebrospinal fluid (CSF) and serum from 998 consecutive neurological patients. Compared with agarose electrophoresis, agarose isoelectric focusing was slightly more sensitive, showing more (and more easily discernible) oligoclonal bands. Agarose isoelectric focusing, which has good reproducibility, revealed oligoclonal bands in CSF in 95% of 43 patients with multiple sclerosis, 44% of 39 with aseptic meningoencephalitis, and 14% of 906 with other neurological diseases. Interestingly, oligoclonal bands were found in CSF from 12% of 162 patients with acute cerebral infarction and 23% of 53 with polyneuropathy, and also in 29% of 17 with dementia, while only 4% of 206 patients with headache, vertigo, or psychoneurosis had this CSF abnormality. We recommend this procedure for the routine examination of paired CSF and serum specimens for the presence of oligoclonal bands.

Chlamydia pneumoniae in the cerebrospinal fluid of patients with multiple sclerosis and neurological controls

2001 ◽  
Vol 7 (6) ◽  
pp. 371-374 ◽  
Author(s):  
S Sotgiu ◽  
A Piana ◽  
M Pugliatti ◽  
A Sotgiu ◽  
G A Deiana ◽  
...  
Keyword(s):  
Multiple Sclerosis ◽  
Central Nervous System ◽  
Cerebrospinal Fluid ◽  
Nervous System ◽  
Chlamydia Pneumoniae ◽  
Immunofluorescence Microscopy ◽  
Antibody Detection ◽  
Common Event ◽  
Neurological Patients ◽  
The Central Nervous System

Chlamydia pneumoniae infection is a common event in neurological patients and recovery of C. pneumoniae DNA in the cerebrospinal fluids (CSF) of multiple sclerosis (MS) patients could represent an epiphenomenon. We assessed the relevance of C. pneumoniae infection in 62 CSF samples from 32 MS patients and 30 neurological controls by means of PCR, immunofluorescence microscopy, enzyme-linked fluorescence and antibody detection. Multiple sclerosis (9.3%) and neurological controls (13.3) had similar percentage of anti-C. pneumoniae antibodies. However, C. pneumoniae DNA was only detectable in MS patients' CSF (9.3%). Our data support the hypothesis that C. pneumoniae persistence in some MS patients may be the result of an impaired clearance within the central nervous system.

Decreased vitamin B12 and folate levels in cerebrospinal fluid and serum of multiple sclerosis patients after high-dose intravenous methylprednisolone

1993 ◽  
Vol 240 (5) ◽  
pp. 305-308 ◽  
Author(s):  
Stephan T. F. M. Frequin ◽  
Ron A. Wevers ◽  
Majorie Braam ◽  
Frederik Barkhof ◽  
Otto R. Hommes
Keyword(s):  
Multiple Sclerosis ◽  
Cerebrospinal Fluid ◽  
Vitamin B12 ◽  
High Dose ◽  
Intravenous Methylprednisolone ◽  
Multiple Sclerosis Patients

Particle-counting immunoassay of a fetuin-like antigen in serum and cerebrospinal fluid.

1985 ◽  
Vol 31 (11) ◽  
pp. 1820-1823 ◽  
Author(s):  
O C Fagnart ◽  
C L Cambiaso ◽  
C J Sindic ◽  
P L Masson
Keyword(s):  
Multiple Sclerosis ◽  
Cerebrospinal Fluid ◽  
Neurological Disorders ◽  
Blood Donors ◽  
Guillain Barre Syndrome ◽  
Particle Counting ◽  
Guillain Barré Syndrome ◽  
Neurological Patients ◽  
Guillain Barré ◽  
Detectable Antigen

Abstract A fetuin-like antigen was detected (smallest concentration detectable: 5 micrograms/L) by particle-counting immunoassay in 2% (13/641) of consecutive patients' sera but not in sera from 80 healthy blood donors, 40 neonates, or 40 pregnant women. The relation of the presence of detectable antigen to patients' diagnosis is not yet clear. However, in the group with cancer (154), it was found only in two of four patients with nephroblastoma and in three of five with tumors of tissue derived from the neurological crest: retinoblastoma (1/1), neuroblastoma (1/3), and medulloblastoma (1/1). Serum specimens from 422 patients with neurological disorders showed the antigen at a concentration greater than 5 micrograms/L in cases of neurosyphilis (5/11), peripheral neuropathy (12/38), Guillain-Barré syndrome (7/27), and multiple sclerosis (74/184). When we assayed 232 specimens of cerebrospinal fluid from the same neurological patients, we found the antigen in two cases of multiple sclerosis (6 and 15 micrograms/L) and in one case of Guillain-Barré syndrome (54 micrograms/L).

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