Peripheral structures of plastids and ultrastructural localization of acid phosphatase and succinic dehydrogenase in a variegated Betula pubescens mutant

1975 ◽  
Vol 53 (10) ◽  
pp. 1072-1077 ◽  
Author(s):  
Niina Valanne
Keyword(s):  
Cell Wall ◽  
Acid Phosphatase ◽  
Succinic Dehydrogenase ◽  
Leaf Tissue ◽  
Ultrastructural Localization ◽  
Betula Pubescens ◽  
High Light ◽  
Electron Microscopic ◽  
White Leaf ◽  
Electron Microscopic Localization

In white leaf tissue from a variegated Betula pubescens mutant, acid-phosphatase activity is seen only in the vacuoles and the cell wall and no precipitation is evident inside the mutant plastids. The electron-microscopic localization of succinic dehydrogenase reveals activity in the intracristal spaces of mitochondria and the envelopes of both mitochondria and plastids. Since precipitation is not apparent inside the protrusions of the plastids and only the membrane occasionally shows a typical mitochondrial reaction, evidence seems to be lacking which indicates that mitochondria originate from plastids. The mitochondrion-like particles budding from the mutant plastids are considered to be proplastids. Peripheral vesicular structures are also seen in the chloroplasts of green leaf tissue from plants growing at high light intensities.

scholarly journals ELECTRON MICROSCOPIC LOCALIZATION OF ACID PHOSPHATASE AND THIAMINE PYROPHOSPHATASE ACTIVITY IN HYPOTHALAMIC NEUROSECRETORY CELLS OF THE RAT

1964 ◽  
Vol 21 (1) ◽  
pp. 35-47 ◽  
Author(s):  
Joseph Osinchak
Keyword(s):  
Acid Phosphatase ◽  
Osmium Tetroxide ◽  
Neurosecretory Cells ◽  
Electron Microscopic ◽  
Vascular Perfusion ◽  
Dense Bodies ◽  
Thiamine Pyrophosphatase ◽  
Pyrophosphatase Activity ◽  
Supraoptic Nuclei ◽  
Electron Microscopic Localization

Supraoptic nuclei in the hypothalamus of rats were fixed for the electron microscope by vascular perfusion with solutions of glutaraldehyde followed by post fixation with osmium tetroxide. Cytochemical methods for detection of acid phosphatase and thiamine pyrophosphatase activity have been applied to glutaraldehyde-fixed frozen sections containing the neurosecretory cells. The enzyme activities have been localized to certain Golgi cisternae. Acid phosphatase activity is present in the large (0.4 µ to 1.0 µ) granules or dense bodies which are surrounded by a single limiting membrane; both features characterize these structures as lysosomes. Smaller (0.1 µ) granules also present in the perikarya are generally unreactive towards enzyme activity and resemble in form the neurosecretory granules in the neurohypophysis.

Intracellular location of enzymes in Rhodopseudomonas spheroides

1968 ◽  
Vol 170 (1020) ◽  
pp. 319-329 ◽  
Keyword(s):  
Cell Wall ◽  
Cytoplasmic Membrane ◽  
Succinic Dehydrogenase ◽  
Particulate Material ◽  
Zinc Protoporphyrin ◽  
Differential Centrifugation ◽  
Electron Microscopic ◽  
Intracellular Location ◽  
Microscopic Studies ◽  
Rhodopseudomonas Spheroides

By differential centrifugation of extracts of pigmented Rhodopseudomonas spheroides a number of constituents, phospholipid and lipid ornithine, and enzymes, zinc protoporphyrin chelatase, succinic dehydrogenase and S-adenosylmethionine-magnesium protoporphyrin methyltransferase, have been found to be associated both with chromatophores and with non-pigmented particulate material. These components are present in both types of material at about the same level. In extracts of non-pigmented organisms the particulate material contains some of the above components, but others are only present in low amounts. The subcellular structures present in the particulate material—ribosomes, cell wall and cytoplasmic membrane—have only been partially separated but, by comparing the distribution of the components listed above with those of known components of ribosomes and cell wall, it is probable that they are associated with cytoplasmic membrane. These studies suggest that the cytoplasmic membrane, apart from lacking the photosynthetic pigments, has a composition similar to that of chromatophores. The data are consistent with the conclusion drawn from electron microscopic studies that chromatophores are derived by invagination of the cytoplasmic membrane.

scholarly journals Correlative light and electron microscopic immunocytochemistry on the same section with colloidal gold.

1987 ◽  
Vol 35 (4) ◽  
pp. 419-425 ◽  
Author(s):  
H Mar ◽  
T Tsukada ◽  
A M Gown ◽  
T N Wight ◽  
D G Baskin
Keyword(s):  
Colloidal Gold ◽  
High Specificity ◽  
Ultrastructural Localization ◽  
Electron Microscopic ◽  
Electron Microscopic Immunocytochemistry ◽  
Antigenic Sites ◽  
Embedding Technique ◽  
Positive Immunostaining ◽  
Arterial Smooth Muscle Cells ◽  
Electron Microscopic Localization

Ultrastructural localization of growth hormone in rat anterior pituitary and of muscle-specific actin in rabbit arterial smooth muscle cells was accomplished with a post-embedment procedure using colloidal gold. Plastic sections (2 microns) were mounted on slides, deplasticized, immunostained with immunoglobulin-colloidal gold particles, re-embedded in Epon, and sectioned for electron microscopy. This procedure enabled light and electron microscopic localization of these intracellular antigens on the same section. Positive immunostaining was demonstrated with this procedure with a muscle-specific actin antibody which previously failed to localize antigenic sites by EM. The procedure described yielded staining of high specificity, with minimal background and well-preserved ultrastructure. This re-embedding technique is useful in situations where problems with post-embedding EM immunostaining exist and where correlative LM and EM immunostaining is essential.

Pollen-Wall Proteins: Electron-Microscopic Localization of Acid Phosphatase in the Intine of Crocus Vernus

1971 ◽  
Vol 8 (3) ◽  
pp. 727-733
Author(s):  
R. B. KNOX ◽  
J. HESLOP-HARRISON
Keyword(s):  
Electron Microscope ◽  
Acid Phosphatase ◽  
Coupling Reaction ◽  
Pollen Grain ◽  
Microscope Level ◽  
Electron Microscopic ◽  
Electron Microscope Level ◽  
Microscopic Localization ◽  
Filamentous Inclusions ◽  
Electron Microscopic Localization

Acid phosphatase has been localized in the wall of the pollen grain of Crocus vernus Wulf at the electron-microscope level by a method using 2-naphthyl thiol phosphate as substrate in a simultaneous coupling reaction with fast blue BBN at pH 5.0, the product being given electron opacity by osmication. Activity was found to be concentrated mainly in the intine, and to be associated with ribbon-like or filamentous inclusions believed to be proteinaceous on the basis of other criteria. Some activity was also detectable in the interstices of the exine. The observations confirm the general interpretation of the distribution of wall-held enzyme based upon light-microscopic cytochemistry, and provide the resolution necessary to establish unambiguously that they are associated with protein layers inserted during intine growth.

Ultracytochemical Localization of Acid Phosphatase in Humicola lutea Conidia and Mycelia

2007 ◽  
Vol 62 (1-2) ◽  
pp. 65-69
Author(s):  
Dimitrina Spasova ◽  
Penka Aleksieva ◽  
Lilyana Nacheva ◽  
Spasimira Radoevska
Keyword(s):  
Cell Wall ◽  
Acid Phosphatase ◽  
Enzymatic Degradation ◽  
Outer Wall ◽  
Wall Layer ◽  
Outer Side ◽  
Electron Microscopic ◽  
Exterior Surface ◽  
The Relationship ◽  
Humicola Lutea

Electron microscopic cytochemical procedures were used to determine the cellular location of acid phosphatase in the fungus Humicola lutea grown in casein-containing medium lacking in mineral orthophosphates. In our investigations acid phosphatase in nongerminating conidia was localized on the outer side of the cell wall, in the cell wall, and on the exterior surface of the plasma membrane. The reaction product of acid phosphatase in germinating conidia was seen in the outer wall layer while in young mycelium on the cell surface and in the exocellular space. The relationship between phosphatase activities localized in the cell wall and their role in the enzymatic degradation of the phosphoprotein casein providing available phosphates for cell growth is discussed.

Freeze-Fracture Replication in the Ultrastructure of Blue-Green Algae

1967 ◽  
Vol 25 ◽  
pp. 74-75
Author(s):  
L. V. Leak
Keyword(s):  
Cell Wall ◽  
Electron Microscope ◽  
Green Algae ◽  
Three Dimensional ◽  
Freeze Fracture ◽  
Electron Microscopic ◽  
Photosynthetic Membranes ◽  
Blue Green Algae ◽  
Cell Biol ◽  
Cellular Components

Electron microscopic observations of freeze-fracture replicas of Anabaena cells obtained by the procedures described by Bullivant and Ames (J. Cell Biol., 1966) indicate that the frozen cells are fractured in many different planes. This fracturing or cleaving along various planes allows one to gain a three dimensional relation of the cellular components as a result of such a manipulation. When replicas that are obtained by the freeze-fracture method are observed in the electron microscope, cross fractures of the cell wall and membranes that comprise the photosynthetic lamellae are apparent as demonstrated in Figures 1 & 2.A large portion of the Anabaena cell is composed of undulating layers of cytoplasm that are bounded by unit membranes that comprise the photosynthetic membranes. The adjoining layers of cytoplasm are closely apposed to each other to form the photosynthetic lamellae. Occassionally the adjacent layers of cytoplasm are separated by an interspace that may vary in widths of up to several 100 mu to form intralamellar vesicles.

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