Effects of nuclear restorer genes on the cytochrome content of corn pollen mitochondria

1976 ◽  
Vol 54 (23) ◽  
pp. 2718-2725 ◽  
Author(s):  
L. S. Watrud ◽  
J. R. Laughnan ◽  
S. J. Gabay ◽  
D. E. Koeppe

Mitochondria were isolated from mature pollen grains of corn (Zea mays L.) having normal (N) or restored Texas male-sterile (TRf) cytoplasm. Comparison of the cytochrome content of the two sources by low-temperature difference spectroscopy indicates that inbred lines carrying nuclear restorer genes and T cytoplasm have an increased cytochrome oxidase content. A lower cytochrome oxidase concentration was found in mitochondria isolated from anthers of nonrestored Texas (cms-T) male-sterile cytoplasm corn than from those isolated from nonexserted anthers of N or TRf plants. Mitochondria in nonsporogenous tissue of cms-T anthers collected just before the time of anthesis of N plants had fewer well defined cristae than mitochondria in comparable anther tissue of N or TRf plants.


1976 ◽  
Vol 56 (2) ◽  
pp. 249-255 ◽  
Author(s):  
PATRICIA SARVELLA ◽  
C. O. GROGAN

The stem morphology of three versions (normal, male-sterile, and restored) of five lines of corn (Zea mays L.) was examined. Corn stems in both the male-sterile (Texas cytoplasm) and restored versions of line Mp466 were shorter than the normal version. Stem diameters were less in the male-sterile version in two of the five lines examined. Histological examination of the three versions in line CI21 showed that vascular bundle radial diameters in cross sections were less in the male-sterile tassel culms than the normal. Reduction in the amount of phloem appeared to be the cause. Cells in longitudinal sections appeared to be about the same length in all versions, or shorter in the normal; therefore, shortening of the male-sterile stems apparently results from a reduction in cell number.









1979 ◽  
Vol 21 (3) ◽  
pp. 417-422 ◽  
Author(s):  
G. J. Scoles ◽  
L. E. Evans

Three inbred lines of rye (Secale cereale L.) known to be capable of restoring fertility to a cytoplasmic male-sterile line were crossed with the sterile line. The proportions of male fertile, partially male fertile and male sterile plants in F2 and backcross progenies indicated that three dominant restorer genes were present in each line. These were designated Rf1, Rf2 and Rf3, their relative expressivity was Rf1>Rf2>Rf3. Expressivity was dependent upon environment. Partial fertility occurred when certain genotypes carried two of the three alleles as dominant, but was dependent upon genotype and environment.







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