A technique for the isolation of intramatrical vesicles from vesicular–arbuscular mycorrhizae
Keyword(s):
A method for isolating the vesicles of vesicular–arbuscular mycorrhizal (VAM) fungi from within the roots of Allium porrum is presented. Colonized roots were homogenized with 0.3 M sucrose – 0.05 M NaHCO3 and filtered through cheesecloth. The supernatant was centrifuged (180 min, 82 000 g) on 1.3 M sucrose – 15 mM CsCl. Our tests yielded 46 000 vesicles per operator day, but the potential yield is limited only by the amount of root material available.
1993 ◽
Vol 39
(6)
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pp. 567-575
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1985 ◽
Vol 15
(6)
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pp. 1061-1064
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1986 ◽
Vol 16
(1)
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pp. 103-108
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