High-frequency embryogenesis in male sterile plants of Brassica napus through microspore culture

1988 ◽  
Vol 66 (8) ◽  
pp. 1676-1680 ◽  
Author(s):  
Phan V. Chuong ◽  
K. P. Pauls ◽  
W. D. Beversdorf
Keyword(s):  
Brassica Napus ◽  
Male Sterility ◽  
Constant Temperature ◽  
Oilseed Rape ◽  
High Frequency ◽  
Microspore Culture ◽  
Doubled Haploids ◽  
Nuclear Genome ◽  
Male Sterile ◽  
Cytoplasmic Male Sterile

Microspores from several Polima cytoplasmic male sterile (Pol-CMS) and Diplotaxis muralis male sterile (Mur-MS) oilseed rape lines were cultured in a modified Nitsch and Nitsch medium in the dark for 4 weeks. High androgenic frequencies were observed in microspore cultures initiated from plants of both male sterile (MS) types. In cultures maintained at a constant temperature (30 °C) 1 or 2% of the microspores from Pol-CMS or Mur-MS lines, respectively, developed into embryos. A greater percentage of the embryos obtained from the Pol-CMS lines were of good quality than those obtained from the Mur-MS lines (25 vs. 5%). Twenty percent of the plants regenerated from embryos of both lines were spontaneous doubled haploids. The results suggest that no unfavorable cytoplasmic–nuclear genome interaction affecting androgenic potential exists in plants exhibiting Polima or Diplotaxis muralis male sterility.

scholarly journals Utilisation of doubled haploids in winter oilseed rape (Brassica napus L.) breeding

2012 ◽  
Vol 38 (No. 1) ◽  
pp. 50-54 ◽  
Author(s):  
V. Kučera ◽  
M. Vyvadilová ◽  
M. Klíma
Keyword(s):  
Brassica Napus ◽  
Oilseed Rape ◽  
Doubled Haploid ◽  
Microspore Culture ◽  
Doubled Haploids ◽  
Winter Oilseed Rape ◽  
Brassica Napus L ◽  
The Czech Republic ◽  
Yield Parameters ◽  
The World

A survey of development and prospects of the utilisation of doubled haploid techniques in rapeseed breeding in the world and in the Czech Republic is presented. The first utilisation of spontaneously occurred haploids from Brassica napus inbreeding programmes is described. The development of techniques of anther and later microspore culture is outlined. Special emphasis is given to the practical use of doubled haploids for the improvement of the effectiveness of breeding new productive cultivars. Some partial results of evaluation of yield parameters and resistance to important diseases in the obtained doubled haploid lines of winter oilseed rape are shown. The literary review and present results indicate, that the doubled haploid technique can be effectively used for the development of homozygous oilseed rape lines as an alternative to conventional methods.

Diplotaxis muralis (mur) cytoplasmic male sterility system maintainer occurrence and frequency in summer rape

2000 ◽  
Vol 80 (3) ◽  
pp. 587-589 ◽  
Author(s):  
Teresio C. Riungu ◽  
Peter B. E. McVetty
Keyword(s):  
Brassica Napus ◽  
Cytoplasmic Male Sterility ◽  
Key Words ◽  
Male Sterility ◽  
Frequency Of Occurrence ◽  
Male Sterile ◽  
Brassica Napus L ◽  
Cytoplasmic Male Sterile

No male sterile plants were found in the F1 progeny from crosses between a Diplotaxis muralis cytoplasmic male sterile (mur CMS) semi-winter habit rape (Brassica napus L.) A-line and 101 genetically diverse summer rape cultivars or strains. The frequency of occurrence of mur CMS system maintainer genotypes in summer rape is very low, possibly zero. Key words: Mur CMS system; Brassica napus L.

scholarly journals Cytological observation of anther structure and genetic investigation of a thermo-sensitive genic male sterile line 373S in Brassica napus L

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Yanyan Sun ◽  
Dongsuo Zhang ◽  
Zhenzhen Wang ◽  
Yuan Guo ◽  
Xiaomin Sun ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Male Sterility ◽  
Spontaneous Mutation ◽  
Hybrid Breeding ◽  
Pcr Analysis ◽  
Cytological Observation ◽  
Male Sterile ◽  
Pollen Abortion ◽  
Genetic Investigation ◽  
Temperature Changes

Abstract Background Photoperiod and/or thermo-sensitive male sterility is an effective pollination control system in crop two-line hybrid breeding. We previously discovered the spontaneous mutation of a partially male sterile plant and developed a thermo-sensitive genic male sterile (TGMS) line 373S in Brassica napus L. The present study characterized this TGMS line through cytological observation, photoperiod/ temperature treatments, and genetic investigation. Results Microscopic observation revealed that the condensed cytoplasm and irregular exine of microspores and the abnormal degradation of tapetum are related to pollen abortion. Different temperature and photoperiod treatments in field and growth cabinet conditions indicated that the fertility alteration of 373S was mainly caused by temperature changes. The effects of photoperiod and interaction between temperature and photoperiod were insignificant. The critical temperature leading to fertility alteration ranged from 10 °C (15 °C/5 °C) to 12 °C (17 °C/7 °C), and the temperature-responding stage was coincident with anther development from pollen mother cell formation to meiosis stages. Genetic analysis indicated that the TGMS trait in 373S was controlled by one pair of genes, with male sterility as the recessive. Multiplex PCR analysis revealed that the cytoplasm of 373S is pol type. Conclusions Our study suggested that the 373S line in B. napus has a novel thermo-sensitive gene Bnmst1 in Pol CMS cytoplasm background, and its fertility alteration is mainly caused by temperature changes. Our results will broaden the TGMS resources and lay the foundation for two-line hybrid breeding in B. napus.

Advancement in CMS Based Hybrid Development in Cauliflower (Brassica oleracea var. Botrytis)

2020 ◽  
pp. 18-24
Author(s):  
Amit Kumar ◽  
Anjani Kumar ◽  
Chandan Roy
Keyword(s):  
Male Sterility ◽  
Somatic Hybridization ◽  
Microspore Culture ◽  
Intermediate Stage ◽  
Restorer Line ◽  
Undesirable Effect ◽  
Male Sterile ◽  
Sib Mating ◽  
Male Sterile Cytoplasm ◽  
Hybrid Development

Among the different mechanism of male sterility operated in the Brassica group crop. Cytoplasmic male sterility mechanism is most suitable for hybrid development in cauliflower because here the curd (intermediate stage) is an edible part of the cauliflower. Further, there is no requirement of restorer line in this case as required in other seed crop. For the multiplication and maintenance of the different lines (A line and B line), sib mating and selfing is not always desirable. In fact, in such situation doubled haploid production through microspore culture is a more appropriate mechanism. Apart from this, the undesirable effect of integration of male sterile cytoplasm can be mitigated by adopting the repeated back crossing, through chloroplast substitution or somatic hybridization mechanism.

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