Antifungal compounds in aspen: effect of water stress

1994 ◽  
Vol 72 (4) ◽  
pp. 454-460 ◽  
Author(s):  
Brian M. Kruger ◽  
Paul D. Manion
Keyword(s):  
Tissue Culture ◽  
Water Stress ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Clonal Variation ◽  
Antifungal Compounds ◽  
Inhibitory Compounds ◽  
Hypoxylon Mammatum ◽  
Layer Chromatography ◽  
Tissue Culture Plantlets

A thin layer chromatography bioassay was used to detect antifungal compounds in tissue culture plantlets and potted seedlings of aspen. Catechol and the phenolic glycosides salicin and salicortin were identified as inhibitory compounds; a fourth compound was observed but was not identified. Inhibitory compound levels were estimated in eight tissue culture clones grown on unamended media and media amended with 0.22 M mannitol to induce water stress. Aspen tissue culture plantlets grown under water stress conditions had significantly lower levels of catechol, salicortin, and salicin. Significant clonal variation in levels of catechol, salicin, and the unidentified compound was also observed. Catechol, salicortin, and salicin were inhibitory to Hypoxylon mammatum when tested at levels similar to those employed in the thin layer chromatography bioassay. These results suggest that a reduction in the levels of inhibitory compounds in water-stressed aspen may be a factor in the water stress induced susceptibility of aspen to H. mammatum. Key words: Populus tremuloides, Hypoxylon mammatum, water stress, tissue culture.

scholarly journals PENGARUH MANIPULASI MEDIA TERHADAP KANDUNGAN ALKALOID VINKRISTINA KALUS DAUN Catharanthus roseus(L.) G.Don

1995 ◽  
Vol 1 (1) ◽  
pp. 1-7
Author(s):  
Y. Sri Wulan Manuhara
Keyword(s):  
Tissue Culture ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Catharanthus Roseus ◽  
Leaf Explants ◽  
Preparative Thin Layer Chromatography ◽  
Uv Spectra ◽  
Basic Medium ◽  
The Media ◽  
Layer Chromatography

The effect of the manipulation of the media on the vincristine alkaloid content in the callus of Catharanthus roseus (L.) G.Don were studied. This work was done as an effort for gaining the vincristine alkaloid through tissue culture which was expected to obtain a larger amount of the alkaloid. Tissue culture of C. roseus was initiated from leaf explants on growth medium (MS supplemented with 1 mg per l 2,4-D and 1 mg per l BAP). after seven weeks incubation, with only subculturing on the same medium, the proliferating calli were subculture on a production medium (MS supplemented with 1mg per l IAA and 1 mg per l BAP) which were containing different additional concentration of sucrose, BAP, tryptophan, and concentration of 50 percent basic medium of MS from the standard. Eleven weeks-old calli were harvested from each treatment and dried for chemical analysed by thin layer chromatography on silica gel GF 254 using chloroform-aceton-triethylamine as mobile phase. Rf value and uv spectra were used to identify vincristine, and concentration of vincristine alkaloid was determine by preparative thin layer chromatography with methanol solvent and measured by uv-vis spectrophotometer at 233 nm. The maximum content of vincristine alkaloid was obtained from callus, which was grown on the MS standard with an addition of 40 g per l sucrose or 4 mg per l BAP or and also 50 percent of the MS standard medium. Tryptophan addition a precursor could not induce the alkaloid vincristine forming.

scholarly journals Detection of antifungal compounds in Arabidopsis thaliana and brassica oleracea by thin layer chromatography .

2017 ◽  
Vol 23 (1&2) ◽  
pp. 123
Author(s):  
K.P. Chong ◽  
J.W. Mansfield ◽  
M.H. Bennet ◽  
J.F. Rossiter
Keyword(s):  
Arabidopsis Thaliana ◽  
Thin Layer ◽  
Brassica Oleracea ◽  
Thin Layer Chromatography ◽  
Pseudomonas Syringae ◽  
Antifungal Compounds ◽  
Zone Of Inhibition ◽  
Layer Chromatography

Thin Layer Chromatography (TLC) was applied to detect antifungal compounds both in Arabidopsis thaliana and Brassica oleracea after elicited by various biotic and abiotic elicitors.From TLC bioassays the only strong zone of inhibition detected after challenged by Pseudomonas syringae pv.maculicola was from Arabidopsis tissue and later confirmed by spectrophotometry as camalexin but no corresponding phytoalexin was found in broccoli leaf.

Thin-Layer Chromatography

1965 ◽  
Author(s):  
H. R. Bolliger ◽  
M. Brenner ◽  
H. Gänshirt ◽  
Helmut K. Mangold ◽  
H. Seiler ◽  
...  
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Layer Chromatography

THE LESS-POLAR METABOLITES PRODUCED BY INCUBATION OF TESTOSTERONE-4-14C WITH RAT AND BOVINE BRAIN

1969 ◽  
Vol 61 (4) ◽  
pp. 641-648 ◽  
Author(s):  
Leon J. Sholiton ◽  
Emile E. Werk
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Paper Chromatography ◽  
Specific Activity ◽  
Bovine Brain ◽  
Polar Fraction ◽  
End Products ◽  
Polar Metabolites ◽  
Layer Chromatography ◽  
Constant Specific Activity

ABSTRACT Rat and bovine brain have been incubated with testosterone-4-14C under standard conditions. With use of paper chromatography, the extracted metabolites were noted to fall into less-polar, iso-polar, and more polar fractions. The components of the less-polar fraction were separated by acetylation and thin-layer chromatography and the major end-products identified by recrystallization to constant specific activity or constant 3H/14C ratios. Androst-4-enedione and 5α-dihydrotestosterone were formed consistently under the conditions utilized. Trace amounts of other less-polar metabolites were noted occasionally.

Sign in / Sign up

Export Citation Format

Share Document