A new approach to the study of apical meristem development using laser scanning confocal microscopy

1998 ◽  
Vol 76 (5) ◽  
pp. 899-904 ◽  
Author(s):  
Gordon D Lemon ◽  
Usher Posluszny
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Confocal Microscopy ◽  
Light Microscopy ◽  
Laser Scanning ◽  
Apical Meristem ◽  
Laser Scanning Confocal Microscopy ◽  
Shoot Apices ◽  
Scanning Confocal Microscopy ◽  
Scanning Electron

Epi-illumination light microscopy and scanning electron microscopy have been standard techniques for developmental studies of shoot apices. Recently, laser scanning confocal microscopy has gained popularity as a tool for biological imaging. We have adapted laser scanning confocal microscopy to study development in whole shoot apices. It was tested on angiosperm and fern apices using three fluorescent dyes; acriflavine, safranin O, and acid fuchsin, and compared with epi-illumination light microscopy and scanning electron microscopy. In all cases, acid fuchsin proved to be the best fluorochrome for examining shoot apices; having a high affinity for cell walls and nuclear material. The images produced with laser scanning confocal microscopy were sharper and clearer than images generated with epi-illumination light microscopy and scanning electron microscopy. Laser scanning confocal microscopy allows one to map patterns of cell division on the surface of an apical meristem, which is extremely difficult using other techniques such as scanning electron microscopy or epi-illumination light microscopy. Since the laser scanning light microscope records images digitally a method for digital plate production is described. Our methods can easily be applied to study the development of other plant structures on a cellular level such as root apical meristems, floral meristems, stomata, or trichomes, and reproductive organs in lower plants.Key words: confocal microscopy, apical meristem, development, fluorochrome, cytokinesis.

Some possibilities of representing microcirculation in human spleen

Biologia ◽  
2009 ◽  
Vol 64 (6) ◽  
Author(s):  
Paulína Gálfiová ◽  
Ivan Varga ◽  
Martin Kopáni ◽  
Peter Michalka ◽  
Jana Michalková ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Laser Scanning ◽  
Three Dimensional ◽  
Laser Scanning Confocal Microscopy ◽  
The Other ◽  
Corrosion Casts ◽  
Histochemical Analysis ◽  
Human Spleen ◽  
Scanning Confocal Microscopy ◽  
Scanning Electron

AbstractThe representation of microcirculation can be approached in several ways. One of the possibilities is to represent the endothelium (endothelial or sinus lining cells) and their basement membrane on the basis of detecting the known components and the expression of the surface antigenes by the methods of immuno-, enzyme- or lectino-histochemical analysis, or by staining or impregnation histological methods. The other possibility is the examination of samples by transmission and scanning electron microscopy. For three-dimensional demonstration corrosion casts techniques or laser scanning confocal microscopy can be used. In this paper we describe the survey of immuno-, enzyme- and lectino-histochemical characteristics of selected components of microcirculation and our own results of its demonstration in human spleen.

Preparation of recombinant human-like collagen/fibroin scaffold and its promoting effect on vascular cells biocompatibility

2018 ◽  
Vol 33 (4) ◽  
pp. 416-425 ◽  
Author(s):  
Jia Yan ◽  
Kun Hu ◽  
YongHao Xiao ◽  
Fan Zhang ◽  
Lu Han ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Vascular Smooth Muscle Cells ◽  
Laser Scanning ◽  
Muscle Cells ◽  
Laser Scanning Confocal Microscopy ◽  
Scanning Confocal Microscopy ◽  
Scanning Electron

A novel recombinant human-like collagen/fibroin scaffold has been prepared previously, which has high porosity, controllable pore size, and much better mechanical properties than the reported fibroin-based scaffold. In this research, the cell responses of vascular smooth muscle cells to this blend scaffold were examined in vitro. Cell morphology, adherence, and growth in scaffolds were observed by scanning electron microscopy, laser scanning confocal microscopy after staining of the cells with propidium iodide at 1, 3, 5, and 7 days, respectively. A wide range of measurements, including 3-[4,5–dimethylthiazol-2-yl]-2, 5-diphenyl tetrasodium bromide assay, and total intracellular protein content at the end of 7 days culture, were conducted. An increase of viability and protein content of vascular smooth muscle cells cultured in recombinant human-like collagen/fibroin scaffold was found. The laser scanning confocal microscopy and scanning electron microscopy results confirm that the cells readily adhered and proliferation in the blend than in fibroin scaffold, and indicate a better adhesion process. The positive effects were especially significant for vascular smooth muscle cells. The recombinant human-like collagen/fibroin scaffold could be a promising biomaterial for vascular tissue engineering.

Organization of pharyngeal hard parts and musculature in Gnathostomula armata (Gnathostomulida: Gnathostomulidae)

2003 ◽  
Vol 81 (9) ◽  
pp. 1463-1470 ◽  
Author(s):  
Martin V Sørensen ◽  
Seth Tyler ◽  
Matthew D Hooge ◽  
Peter Funch
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Light Microscopy ◽  
Laser Scanning ◽  
Laser Scanning Microscopy ◽  
Basal Plate ◽  
Confocal Laser ◽  
Pharyngeal Bulb ◽  
Additional Information ◽  
Scanning Electron

The pharynx of Gnathostomula armata, like that of other members of the phylum Gnathostomulida, consists of a set of jaws, a basal plate, and a muscular bulb that encloses these cuticularized hard parts. Field-emission scanning electron microscopy (SEM) provides additional information about the hard parts and shows that the dentition of the jaws is arranged in three rows: 7–10 teeth in a dorsal row, 16–20 teeth in a medial row, and 20 teeth in a ventral row, a pattern different from that reported from light microscopy (LM). SEM also shows that the dentition of the basal plate is more like that of other Gnathostomula species than was previously discerned. Confocal laser scanning microscopy shows the musculature of the pharyngeal bulb to comprise diductors that open and tilt the jaws, looplike abductors that retract them as they snap shut by recoil, and a pair of inclinators and pair of levators that also participate in tilting the jaws back and forth. A constrictor running ventral to and behind the jaws may work to protrude them. Two arc-shaped muscles attached to the basal plate pull it forward and tilt it down to scrape food from the substratum so that it can be grabbed by the jaws. Paired retractor muscles pull the basal plate back into the mouth.

scholarly journals The micromorphological, histochemical and confocal analysis of satureja subspicata Bartl. ex vis. glandular trichomes

2010 ◽  
Vol 62 (4) ◽  
pp. 1143-1149 ◽  
Author(s):  
Marija Marin ◽  
N. Jasnic ◽  
D. Lakusic ◽  
Sonja Duletic-Lausevic ◽  
Lia Ascensao
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Light Microscopy ◽  
Positive Reaction ◽  
Laser Scanning ◽  
Glandular Trichomes ◽  
Confocal Laser Scanning ◽  
Confocal Laser ◽  
Histochemical Tests ◽  
Scanning Electron

Micromorphology, histochemical and confocal analyses of the trichomes of Satureja subspicata (Bartl. ex Vis.) were carried out using light microscopy, confocal laser scanning electron microscopy (CLSM), and scanning electron microscopy. Non-glandular unbranched and two types of glandular trichomes - peltate and capitate - are described. The results of histochemical tests showed a positive reaction to phenolics, tannins, lipids, acid lipids, pectins and polysaccharides in both types of glandular trichomes. A strong red autofluorescence of the lipophilic and hydrophilic secreted material in glandular trichomes was observed with CLSM.

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