scholarly journals The micromorphological, histochemical and confocal analysis of satureja subspicata Bartl. ex vis. glandular trichomes

2010 ◽  
Vol 62 (4) ◽  
pp. 1143-1149 ◽  
Author(s):  
Marija Marin ◽  
N. Jasnic ◽  
D. Lakusic ◽  
Sonja Duletic-Lausevic ◽  
Lia Ascensao

Micromorphology, histochemical and confocal analyses of the trichomes of Satureja subspicata (Bartl. ex Vis.) were carried out using light microscopy, confocal laser scanning electron microscopy (CLSM), and scanning electron microscopy. Non-glandular unbranched and two types of glandular trichomes - peltate and capitate - are described. The results of histochemical tests showed a positive reaction to phenolics, tannins, lipids, acid lipids, pectins and polysaccharides in both types of glandular trichomes. A strong red autofluorescence of the lipophilic and hydrophilic secreted material in glandular trichomes was observed with CLSM.

2008 ◽  
Vol 60 (4) ◽  
pp. 667-672 ◽  
Author(s):  
Marija Marin ◽  
Snezana Budimir ◽  
Dusica Janosevic ◽  
P.D. Marin ◽  
Sonja Duletic-Lausevic ◽  
...  

Micromorphology, distribution, and histochemistry of the trichomes of Thymus lykae were studied using scanning electron microscopy (SEM)and confocal laser scanning electron microscopy (CLSM). The leaves, stem, and calyx bear numerous glandular and non-glandular trichomes. Two types of glandular trichomes are identified - peltate and capitate. Results of histochemical tests showed positive reactions to polysaccharides, proteins, and lipids. Yellow autofluorescence of secreted material was noticed in peltate and capitate trichomes. Strong reddish-yellow autofluorescence of the lipophilic and hydrophilic secreted material was observed with CLSM.


2009 ◽  
Vol 72 (6) ◽  
pp. 447-453
Author(s):  
Antonio Madroñero De La Cal ◽  
Juan Aguado-Serrano ◽  
Maria Luisa Rojas-Cervantes ◽  
Elena V. Rosa Adame ◽  
Belen Sarmiento Marron ◽  
...  

2003 ◽  
Vol 81 (9) ◽  
pp. 1463-1470 ◽  
Author(s):  
Martin V Sørensen ◽  
Seth Tyler ◽  
Matthew D Hooge ◽  
Peter Funch

The pharynx of Gnathostomula armata, like that of other members of the phylum Gnathostomulida, consists of a set of jaws, a basal plate, and a muscular bulb that encloses these cuticularized hard parts. Field-emission scanning electron microscopy (SEM) provides additional information about the hard parts and shows that the dentition of the jaws is arranged in three rows: 7–10 teeth in a dorsal row, 16–20 teeth in a medial row, and 20 teeth in a ventral row, a pattern different from that reported from light microscopy (LM). SEM also shows that the dentition of the basal plate is more like that of other Gnathostomula species than was previously discerned. Confocal laser scanning microscopy shows the musculature of the pharyngeal bulb to comprise diductors that open and tilt the jaws, looplike abductors that retract them as they snap shut by recoil, and a pair of inclinators and pair of levators that also participate in tilting the jaws back and forth. A constrictor running ventral to and behind the jaws may work to protrude them. Two arc-shaped muscles attached to the basal plate pull it forward and tilt it down to scrape food from the substratum so that it can be grabbed by the jaws. Paired retractor muscles pull the basal plate back into the mouth.


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