A molecular phylogenetic reappraisal of theGraphiumcomplex based on 18S rDNA sequences

1998 ◽  
Vol 76 (9) ◽  
pp. 1495-1506 ◽  
Author(s):  
Gen Okada ◽  
Keith A Seifert ◽  
Akiko Takematsu ◽  
Yuichi Yamaoka ◽  
Satoru Miyazaki ◽  
...  
Keyword(s):  
18S Rdna ◽  
Small Subunit ◽  
Ssu Rdna ◽  
New Combinations ◽  
Rdna Sequences ◽  
As Species ◽  
Molecular Phylogenetic ◽  
Molecular Context ◽  
18S Rdna Sequences ◽  
Mode Characteristic

Based on nuclear encoded small subunit (18S) rDNA sequences, a taxonomic reappraisal of Graphium (anamorphic fungi) was undertaken using neighbour-joining (NJ) and fast DNA maximum likelihood (fastDNAml) methods and compared with traditional classifications. In common with Graphium putredinis, Graphium penicillioides (the lectotype species) was found to be related to the Microascales, not the Ophiostomatales as previously believed. Both species might be heterogenous and should be treated as species aggregates. The representative mode of conidiogenesis for these two species was nodular-annellidic, rather than the dense-annellidic mode characteristic of the synnematous ophiostomatalean anamorphs. Graphium is emended to be restricted to G. penicillioides, G. putredinis, and related synnematous anamorphs of Petriella and Pseudallescheria, and a nomenclator for the nine species presently accepted in Graphium is presented. Pesotum, originally characterized mainly by sympodial conidiogenesis, is emended to include synnematous anamorphs of Ophiostoma species formerly included in a variety of genera with sympodial to dense-annellidic conidiogenesis. Eight new combinations in Pesotum are included in a nomenclator for the 26 species currently known. Three new combinations from Ceratocystis to Ophiostoma are proposed for species with Pesotum anamorphs. The holomorph of Graphium calicioides has affinities to the black yeasts and should be classified in the Chaetothyriales. However, the critical morphological, loculoascomycetous characters of the teleomorph are not completely documented. Interpreted from the molecular context, the morphological similarities between these three groups of anamorphs are homoplasies and examples of convergent evolution.Key words: Chaetothyriales, Graphium, Microascales, Ophiostomatales, Pesotum, 18S (SSU) rDNA sequences.

A molecular phylogenetic reappraisal of the Graphium complex based on 18S rDNA sequences

1998 ◽  
Vol 76 (9) ◽  
pp. 1495-1506 ◽  
Author(s):  
Gen Okada ◽  
Keith A. Seifert ◽  
Akiko Takematsu ◽  
Yuichi Yamaoka ◽  
Satoru Miyazaki ◽  
...  
Keyword(s):  
18S Rdna ◽  
Rdna Sequences ◽  
Molecular Phylogenetic ◽  
18S Rdna Sequences

scholarly journals Taxonomic characterization of ten Thraustochytrids strains isolated from mangrove Xuan Thuy, Nam Dinh

2016 ◽  
Vol 14 (2) ◽  
pp. 377-384
Author(s):  
Nguyễn Thị Hoài Hà ◽  
Phạm Thị Bích Đào ◽  
Nguyễn Đình Tuấn
Keyword(s):  
Cell Division ◽  
18S Rdna ◽  
Rdna Sequences ◽  
Marine Habitats ◽  
The Family ◽  
Molecular Phylogenetic ◽  
Binary Division ◽  
18S Rdna Sequences ◽  
Amoeboid Cells

Thraustochytrids have become of considerable industrial and scientific interest in the past decade due to their health benefits. Thraustochytrids are found in a wide variety of marine habitats such as the coastal, mangrove and sediments including the deep sea. Thraustochytrids are extremely common on the detritus, macroalgae and decaying leaf, they play an important role as organic matter-degrading microorganisms Thraustochytrids are unicellular, eukaryotic, chemo-organotrophic organisms. Ten thraustochytrids strains PT269, PT270, PT273, PT274, PT279, PT284, PT285, PT287, PT81, PT84 were isolated from four locations in Xuan Thuy mangroves, Nam Dinh. In this report, classification is based on morphology and 18S rDNA sequences. Ten Thraustochytrid strains could be classified into three types of colony and four types of cell morphology. Molecular phylogenetic analysis of 18S rDNA sequences showed homology score to be 99-100% and these strains belonged to four genera in the family Thraustochytriaceae. PT269, PT279, PT284 and PT287 strains belong to Aurantiochytrium genus, they produce amoeboid cells and occur successive binary division. PT273 and PT285 strains belong to Thraustochytrium genus, thallus directly develop and cleave into sporangium. PT274 strain belong to Aplanochytrium genus with two distinct development, amoeboid cells are found, they rapidly round up and become sporangium; and successive binary cell division. PT270, PT81 and PT84 strains belong to genus Schizochytrium, they have successive binary cell division, zoospores release.

Myxobolus jialingensis n. sp. (Myxozoa: Myxobolidae) infecting urinary bladder and hepatopancreas of yellowhead catfish Tachysurus fulvidraco from China

Zootaxa ◽  
2020 ◽  
Vol 4819 (1) ◽  
pp. 179-186
Author(s):  
LEI GAO ◽  
JING ZHANG ◽  
CHENGZHONG YANG ◽  
YUANJUN ZHAO
Keyword(s):  
Urinary Bladder ◽  
18S Rdna ◽  
Polar Capsule ◽  
Small Subunit ◽  
Rdna Sequences ◽  
Multiple Origins ◽  
Close Phylogenetic Relationship ◽  
18S Rdna Sequences ◽  
Tachysurus Fulvidraco ◽  
Myxosporean Species

In the present study, we described a novel myxosporean species, Myxobolus jialingensis n. sp. (Myxozoa: Myxobolidae), which infected the urinary bladder and hepatopancreas of yellowhead catfish Tachysurus fulvidraco in China. The mature spores of M. jialingensis n. sp. were pyriform with the length of 15.8 ± 0.7 (15.4–17.0) μm and width of 8.0 ± 0.3 (7.8–8.9) μm. Two pyriform polar capsules were slightly unequal in size: the larger polar capsule was 7.4 ± 0.3 (6.7–8.0) μm in length and 3.1 ± 0.2 (2.8–3.6) μm in width; and the smaller polar capsule measured 7.3 ± 0.3 (6.6–8.1) μm in length and 3.3 ± 0.2 (2.9–3.6) μm in width. The polar capsules were directed toward the apex of the spore, packing seven to eight spirals of the polar filaments. The small subunit ribosomal RNA gene (18S rDNA) sequence of M. jialingensis n. sp. was unique among all myxozoans, and the highest similarity was 96.1% with M. voremkhai. Phylogenetic analysis based on 18S rDNA sequences revealed that myxosporeans infecting the close host affinity (belonging to the same order) had close phylogenetic relationship and, some myxosporeans infecting the same host order might have multiple origins.

Molecular Phylogenetic Relationships Among Angiosperms: An Overview Based on rbcL and 18S rDNA Sequences

1997 ◽  
pp. 157-178 ◽  
Author(s):  
Douglas E. Soltis ◽  
Carola Hibsch-Jetter ◽  
Pamela S. Soltis ◽  
Mark W. Chase ◽  
James S. Farris
Keyword(s):  
Phylogenetic Relationships ◽  
18S Rdna ◽  
Rdna Sequences ◽  
Molecular Phylogenetic ◽  
18S Rdna Sequences

scholarly journals A molecular perspective on generic concepts in the Hypotrachyna clade (Parmeliaceae, Ascomycota)

Phytotaxa ◽  
2013 ◽  
Vol 132 (1) ◽  
pp. 21 ◽  
Author(s):  
PRADEEP K. DIVAKAR ◽  
ANA CRESPO ◽  
JANO NÚÑEZ-ZAPATA ◽  
ADAM FLAKUS ◽  
HARRIE J.M. SIPMAN ◽  
...  
Keyword(s):  
Molecular Phylogeny ◽  
Phylogenetic Analyses ◽  
Ssu Rdna ◽  
New Combinations ◽  
Generic Delimitation ◽  
Rdna Sequences ◽  
Phylogenetic Studies ◽  
Molecular Phylogenetic ◽  
Alternative Hypotheses ◽  
Nuclear Its

Recently, molecular phylogenetic studies have revolutionized the generic concepts in Parmeliaceae and in lichen forming fungi in general. In the present study, the generic delimitation in the Hypotrachyna clade is revised using a molecular phylogeny of nuclear ITS, LSU and mitochondrial SSU rDNA sequences of 88 hypotrachynoid taxa. Morphological and chemical features are also revised in each group. 118 sequences are newly generated for this study. Our phylogenetic analyses show the polyphyly of Hypotrachyna as currently circumscribed which falls into four well-supported and one unsupported clade. Cetrariastrum, Everniastrum and Parmelinopsis are nested within Hypotrachyna s. lat., Parmelinopsis being also polyphyletic and nested in one of the Hypotrachyna clades. Cetrariastrum is monophyletic but clustered within Everniastrum. Two alternative hypotheses tests significantly rejected the monophyly of these three genera. As a consequence, the genera Cetrariastrum, Everniastrum, and Parmelinopsis are reduced to synonymy with Hypotrachyna. Furthermore, we here propose an alternative classification to recognize the well-supported clades at subgeneric level and leave the remaining species unclassified within the genus. Five new subgenera are proposed: Hypotrachyna subgen. Cetrariastrum, Hypotrachyna subgen. Everniastrum, Hypotrachyna subgen. Longilobae, Hypotrachyna subgen. Parmelinopsis, and Hypotrachyna subgen. Sinuosae. Forty-nine new combinations are proposed.

scholarly journals Molecular phylogenetic analyses of tissue coccidia (sarcocystidae; apicomplexa) based on nuclear 18s RDNA and mitochondrial COI sequences confirms the paraphyly of the genus Hammondia

2016 ◽  
Vol 2 ◽  
Author(s):  
MOSUN E. OGEDENGBE ◽  
JOSEPH D. OGEDENGBE ◽  
JULIA C. WHALE ◽  
KRISTIN ELLIOT ◽  
MARCO A. JUÁREZ-ESTRADA ◽  
...  
Keyword(s):  
18S Rdna ◽  
Neospora Caninum ◽  
Phylogenetic Analyses ◽  
Mitochondrial Coi ◽  
Rdna Sequences ◽  
Molecular Phylogenetic ◽  
18S Rdna Sequences ◽  
Phylogenetic Hypotheses ◽  
Coi Sequences ◽  
Sarcocystis Rileyi

SUMMARYPartial mitochondrial cytochromecoxidase subunit I (mt COI) sequences were generated from:Toxoplasma gondii(strains CTG, GTI, MAS, ME49, PTG, TgCatBr5, TgCat, Br64, TgCgCal, TgToucan);Neospora caninum(Strain NC1);Hammondia hammondi(Strain H.H–20);H. heydorni; H. cf.triffittae; Cystoisospora felis; C. suis; C. canis; C. rivolta; C. cf.ohioensis; Caryospora bigenetica; Sarcocystis rileyi; andS. neurona. Nuclear 18S rDNA sequences were generated forH. heydorni, H. hammondi, C. suis, C. canis, C. felis, C. rivolta, C. cf.ohioensis, S. neurona, andS. rileyi. Aligned, concatenated 18S rDNA and COI sequences were Bayesian analysed using partitioned nucleotide substitution models [HKY + I + G for 18S; GTR + I + G codon (code = metmt) for COI]. Phylogenetic hypotheses supported a monophyletic Sarcocystidae and its subfamilie with two major clades within the Toxoplasmatinae: (1) a monophyletic clade ofCystoisosporaspp. withNephroisospora eptesici; and (2) a clade ofToxoplasma, NeosporaandHammondia. Within the latter,Hammondiawas shown to be paraphyletic;H. heydorniandH. triffittaewere monophyletic withN. caninum[canine definitive hosts (DHs)], whereasH. hammondiwas monophyletic withT. gondii(feline DHs). A new genus is erected to resolve the paraphyly of the genusHammondiaconfirmed using mt COI and combined 18S/COI sequence datasets.

Morphological and molecular characterisation of Strongyloides ransomi (Nematoda: Strongyloididae) collected from domestic pigs in Bac Giang province, Vietnam

2017 ◽  
Vol 39 (3) ◽  
pp. 270-275 ◽  
Author(s):  
Nguyen Thi Huong Giang ◽  
Tran Duc Hoan ◽  
Nguyen Thi Thu Huyen ◽  
Nguyen Thi Kim Lan ◽  
Pham Ngoc Doanh
Keyword(s):  
18S Rdna ◽  
Molecular Data ◽  
Parasitic Nematodes ◽  
Molecular Characterisation ◽  
Human Beings ◽  
Domestic Pigs ◽  
Rdna Sequences ◽  
Large Numbers ◽  
Molecular Phylogenetic ◽  
18S Rdna Sequences

The genus Strongyloides is a group of parasitic nematodes of vertebrates comprised of over 50 species. A large numbers of nucleotide sequences of Strongyloides species have been deposited in GenBank. The vast majority of them are of Strongyloides from human beings, non-human primates, rats and cattle, but there are few molecular data of Strongyloides species isolated from pigs. The aim of this study is to identify Strongyloides species collected from pigs from Bac Giang Province, Vietnam, and analyze their molecular phylogenetic relationship within the genus Strongyloides based on 18S rDNA sequences. The morphological and molecular analyses revealed that the Strongyloides samples collected from pigs from Bac Giang Province, Vietnam, were in fact S. ransomi. The 18S rDNA sequences of S. ransomi from Vietnam were completely identical with that from Cambodia, but slightly different (0.3%) from that of Japan. In comparison to other Strongyloides species, S. ransomi was genetically close to S. venezuelensis. Citation: Nguyen Thi Giang, Tran Duc Hoan, Nguyen Thi Thu Huyen, Nguyen Thi Kim Lan, Phạm Ngọc Doanh, 2017. Morphological and molecular characterisation of Strongyloides ransomi (Nematoda: Strongyloididae) collected from domestic pigs in Bac Giang province, Vietnam. Tap chi Sinh hoc, 39(3): 270- 275. DOI: 10.15625/0866-7160/v39n3.10796.*Corresponding author: [email protected] 18 May 2017, accepted 20 August 2017 

Bivalvulidan (Myxozoa: Myxosporea) parasites of damselfishes with description of twelve novel species from Australia's Great Barrier Reef

Parasitology ◽  
2008 ◽  
Vol 135 (10) ◽  
pp. 1165-1178 ◽  
Author(s):  
N. L. GUNTER ◽  
R. D. ADLARD
Keyword(s):  
Novel Species ◽  
Phylogenetic Analyses ◽  
Single Species ◽  
Small Subunit ◽  
Ssu Rdna ◽  
Fish Host ◽  
Multiple Infections ◽  
Rdna Sequences ◽  
Molecular Phylogenetic ◽  
Host Taxon

SUMMARYBivalvulidan parasites from the gall bladder of 31 species of damselfishes (family Pomacentridae) were examined for their taxonomic identity and their relatedness to other species of myxozoans. This paper describes 11 novel ceratomyxid species and a novel Myxidium sp. Each species is characterized morphologically and small subunit (SSU) rDNA sequences were used in molecular phylogenetic analyses. Five pomacentrid species were found to harbour multiple infections of bivalvulidan species. One species of Ceratomyxa and Myxidium were found to infect more than a single species of damselfish. Phylogenetic analyses revealed there has been no radiation of ceratomyxids that can be associated with the fish host taxon and that Myxidium queenslandicus n.sp. was more closely related to Zschokkella mugilis and Ellipsomyxa gobii than other members of the genus Myxidium.

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