Effect of age and temperature on amino acid composition and the content of different protein types of juvenile Atlantic cod (Gadus morhua) otoliths

2004 ◽  
Vol 61 (6) ◽  
pp. 1012-1020 ◽  
Author(s):  
K Hüssy ◽  
H Mosegaard ◽  
F Jessen
Keyword(s):  
Amino Acid ◽  
Amino Acid Composition ◽  
Acid Composition ◽  
Soluble Protein ◽  
Protein Fraction ◽  
Gadus Morhua ◽  
Atlantic Cod ◽  
Water Soluble ◽  
Soluble Protein Fraction ◽  
Water Soluble Protein

The purpose of this study was to analyse the amino acid composition of otolith matrix protein, estimate the proportion of the water-soluble protein fraction, and analyse the effect of matrix composition on otolith visual appearance. Juvenile Atlantic cod (Gadus morhua) were reared under constant temperature and feeding conditions and sampled at the beginning and the end of the experiment. The amino acid composition was dominated by asparagine, glutamic acid, leucine, serine, and proline. A change in amino acid composition was observed with increasing temperature and time, caused by changing proportions of the water-soluble and -insoluble protein fractions. Feeding level had no effect. The relative content of water-soluble protein was linearly related to fish dry weight and temperature. Otolith opacity, defined as the percentage of incident light absorbed by an otolith section, did not differ significantly between experimental treatments. The soluble protein fraction had a positive, albeit insignificant, correlation with opacity. Using opacity and otolith volume, deposited total otolith protein content was estimated with an R2 of 0.91, where otolith volume alone explained 83% of the observed variation.

scholarly journals ANTIBODY STUDIES IN RABBIT ENCEPHALOMYELITIS INDUCED BY A WATER-SOLUBLE PROTEIN FRACTION OF RABBIT CORD

1962 ◽  
Vol 116 (6) ◽  
pp. 807-825 ◽  
Author(s):  
Robert F. Kibler ◽  
Arthur E. Barnes
Keyword(s):  
Soluble Protein ◽  
Protein Fraction ◽  
Nervous Tissue ◽  
Serum Antibody ◽  
Paper Electrophoresis ◽  
Water Soluble ◽  
Soluble Protein Fraction ◽  
Water Soluble Protein ◽  
Antigen Antibody ◽  
Suitable Technique

A water-soluble protein fraction of nervous tissue was prepared by extraction of rabbit spinal cord with sodium citrate at pH 4.3. Characterization by nitrogen content and paper electrophoresis showed it to be a mixture of basic proteins. This extract demonstrated encephalitogenic activity when injected into rabbits. The most suitable technique for the measurement of serum antibody to the rabbit cord antigen proved to be the precipitation of antigen-antibody complexes by 40 per cent saturated ammonium sulfate. Antibody could not be demonstrated by the techniques of complement fixation, quantitative precipitation, and Ouchterlony plates. The early appearance of circulating antibody occurred almost exclusively in rabbits that subsequently developed EAE. Specificity of the antibodies for nervous tissue was demonstrated by appropriate blocking and adsorption experiments.

Two-Dimensional Electrophoretic Study of the Water-Soluble Protein Fraction in White Muscle of Gadoid Fish Species

1998 ◽  
Vol 46 (10) ◽  
pp. 3991-3997 ◽  
Author(s):  
C. Piñeiro ◽  
J. Barros-Velázquez ◽  
C. G. Sotelo ◽  
R. I. Pérez-Martín ◽  
J. M. Gallardo
Keyword(s):  
Fish Species ◽  
Soluble Protein ◽  
Protein Fraction ◽  
White Muscle ◽  
Water Soluble ◽  
Two Dimensional ◽  
Soluble Protein Fraction ◽  
Electrophoretic Study ◽  
Water Soluble Protein

A trypsin inhibitor from the water-soluble protein fraction of wheat kernel

1989 ◽  
Vol 28 (5) ◽  
pp. 1307-1311 ◽  
Author(s):  
Elia Poerio ◽  
Lucia Carrano ◽  
Anna Maria Garzillo ◽  
Vincenzo Buonocore
Keyword(s):  
Trypsin Inhibitor ◽  
Soluble Protein ◽  
Protein Fraction ◽  
Water Soluble ◽  
Soluble Protein Fraction ◽  
Wheat Kernel ◽  
Water Soluble Protein

NATURE OF PROTEINS IN TRITICALE AND ITS PARENTAL SPECIES: I. SOLUBILITY CHARACTERISTICS AND AMINO ACID COMPOSITION OF ENDOSPERM PROTEINS

1970 ◽  
Vol 50 (1) ◽  
pp. 9-14 ◽  
Author(s):  
C. H. CHEN ◽  
W. BUSHUK
Keyword(s):  
Amino Acid ◽  
Durum Wheat ◽  
Amino Acid Composition ◽  
Acid Composition ◽  
Spring Wheat ◽  
Water Soluble ◽  
The Other ◽  
Hard Red Spring Wheat ◽  
Endosperm Proteins ◽  
Spring Wheat Cultivar

Solubility characteristics and amino acid composition of the endosperm proteins of one line of Triticale, its durum wheat and rye parent cultivars, and one cultivar of hard red spring wheat were compared. Quantitative distribution of the soluble protein fractions and amino acid compositions showed that the proteins of Triticale are intermediate in these properties between analogous properties of the proteins of its durum wheat and rye parents. The major differences between the hard red spring wheat and the other three species were its lower content of water-soluble proteins and higher content of insoluble or gluten proteins. This appears lo be the main reason for the superior breadmaking quality of the hard red spring wheat cultivar compared with the other species used in this study.

scholarly journals MICROTUBULE PROTEIN

1971 ◽  
Vol 51 (1) ◽  
pp. 138-147 ◽  
Author(s):  
Howard Feit ◽  
Gary R. Dutton ◽  
Samuel H. Barondes ◽  
Michael L. Shelanski
Keyword(s):  
Amino Acid ◽  
Amino Acid Composition ◽  
Acid Composition ◽  
Gel Electrophoresis ◽  
Soluble Protein ◽  
Peptide Mapping ◽  
Nerve Endings ◽  
Subunit Protein ◽  
Microtubule Protein ◽  
Slow Transport

The subunit protein of microtubules, tubulin, has been demonstrated to be present in isolated nerve endings by gel electrophoresis, amino acid composition, and peptide mapping. The tubulin constitutes approximately 28% of the soluble protein of the nerve endings. The transport of tubulin to the nerve endings has been demonstrated and its relationship to slow transport is discussed.

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