Statolith microchemistry as a technique for discriminating among Great Lakes sea lamprey (Petromyzon marinus) spawning tributaries

2008 ◽  
Vol 65 (6) ◽  
pp. 1153-1164 ◽  
Author(s):  
Carrol P. Hand ◽  
Stuart A. Ludsin ◽  
Brian J. Fryer ◽  
J. Ellen Marsden
Keyword(s):  
Great Lakes ◽  
Inductively Coupled Plasma ◽  
Fishery Management ◽  
Petromyzon Marinus ◽  
Sea Lamprey ◽  
Inductively Coupled ◽  
Elemental Concentrations ◽  
Icp Ms ◽  
Plasma Mass Spectrometry ◽  
Spawning Tributaries

Laurentian Great Lakes fishery management agencies are seeking ways to identify natal origins of parasitic- and spawning-phase sea lamprey ( Petromyzon marinus ) so that efforts to control this invasive species can be prioritized. We developed laser-ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) as a technique to quantify elemental concentrations in larval sea lamprey statoliths and explored the use of statolith microchemistry as a tool to discriminate among larval sea lamprey production streams. Our analyses demonstrate that (i) traversing across the statolith with the laser is preferable to drilling down through its apex, (ii) preserving specimens in 95% ethanol versus freezing them has minimal effects on elemental concentrations, (iii) a minimum of 15 individuals per stream should accurately depict stream-specific statolith elemental signatures, and (iv) LA-ICP-MS is preferable to particle-induced X-ray emission (PIXE) for statolith analysis, based on higher precision, lower cost, reduced sampling-time requirements, and wider availability. Using LA-ICP-MS, we could discriminate among larvae from 13 streams located in Lakes Michigan, Huron, and Superior with 82% classification accuracy, indicating that this tool holds promise for determining natal origins of sea lamprey in the Great Lakes.

Instability of statolith elemental signatures revealed in newly metamorphosed sea lamprey (Petromyzon marinus)

2013 ◽  
Vol 70 (4) ◽  
pp. 565-573 ◽  
Author(s):  
Aude Lochet ◽  
J. Ellen Marsden ◽  
Brian J. Fryer ◽  
Stuart A. Ludsin
Keyword(s):  
Great Lakes ◽  
Inductively Coupled Plasma ◽  
Petromyzon Marinus ◽  
Sea Lamprey ◽  
Laurentian Great Lakes ◽  
Lake Champlain ◽  
Inductively Coupled ◽  
Natal Origin ◽  
Plasma Mass Spectrometry ◽  
The Stability

Techniques that use calcified structures to identify the natal origin of organisms assume that reworking of previously deposited material does not occur. While verified for otoliths, this assumption remains unverified for statoliths. Herein, we test the stability of sea lamprey (Petromyzon marinus) statolith microchemistry during metamorphosis. Using laser ablation inductively coupled plasma mass spectrometry, we quantified the concentrations of nine elements in statoliths from sea lamprey larvae (n = 118) and newly metamorphosed (n = 115) individuals that were collected across six tributaries of varying alkalinity in the Laurentian Great Lakes and Lake Champlain basins. In newly metamorphosed individuals from all streams, the portion of the statolith deposited during the larval stage was enriched in rubidium (Rb), strongly suggesting a chemical reworking of statoliths during metamorphosis. As the ability to discriminate among sea lamprey from different streams in the Great Lakes and Lake Champlain mostly relies on premetamorphic levels of Rb, strategies for the use of statoliths to identify the natal origin of sea lamprey parasitic juveniles and spawners (postmetamorphic stages) should take into account the chemical changes associated with metamorphosis.

scholarly journals Changes in environmental salinity during the life of Pangasius krempfi in the Mekong Delta (Vietnam) estimated from otolith Sr : Ca ratios

2019 ◽  
Vol 70 (12) ◽  
pp. 1734 ◽  
Author(s):  
Ngan T. Tran ◽  
Maylis Labonne ◽  
Huy D. Hoang ◽  
Jacques Panfili
Keyword(s):  
Inductively Coupled Plasma ◽  
Fishery Management ◽  
Mekong Delta ◽  
Mekong River ◽  
Migration Patterns ◽  
Inductively Coupled ◽  
Low Salinity ◽  
Icp Ms ◽  
Plasma Mass Spectrometry ◽  
Commercially Important

Pangasius krempfi is a commercially important catfish in the Mekong River and is believed to migrate along the Mekong River basin. To verify this migration, elemental concentrations were measured in the water and in otoliths to infer the salinity of the water through the fish’s lifetime. In 2017, eight element concentrations were measured along the Mekong Delta using solution-based inductively coupled plasma mass spectrometry (ICP-MS). Concentrations of Sr, Li and Rb were strongly and positively correlated with salinity. Otoliths were taken from P. krempfi caught in the brackish waters of the lower Mekong Delta and seven element:Ca ratios were measured from the core to the otolith edge using laser ablation ICP-MS. The Sr:Ca, Ba:Ca, P:Ca and Mn:Ca ratios varied through the lifetime of the fish, but only Sr:Ca was suitable for estimating ambient salinity. The Sr:Ca profiles in otoliths were analysed and significantly correlated between individuals, with all fish hatched in water with very low levels of salinity, indicating a single freshwater spawning ground, and then living in waters with higher salinity, with two types of migration behaviour. Some individuals may return to low-salinity waters when older. These conclusions were supported by the Ba:Ca and Mn:Ca ratios. These migration patterns may have implications for fishery management.

Single Particle Inductively Coupled Plasma Mass Spectrometry: Investigating Nonlinear Response Observed in Pulse Counting Mode and Extending the Linear Dynamic Range by Compensating for Dead Time Related Count Losses on a Microsecond Timescale

2019 ◽  
Author(s):  
Ingo Strenge ◽  
Carsten Engelhard
Keyword(s):  
Mass Spectrometry ◽  
Inductively Coupled Plasma ◽  
Nonlinear Response ◽  
Dynamic Range ◽  
Dead Time ◽  
Inorganic Nanoparticles ◽  
Linear Dynamic Range ◽  
Inductively Coupled ◽  
Icp Ms ◽  
Plasma Mass Spectrometry

<p>The article demonstrates the importance of using a suitable approach to compensate for dead time relate count losses (a certain measurement artefact) whenever short, but potentially strong transient signals are to be analysed using inductively coupled plasma mass spectrometry (ICP-MS). Findings strongly support the theory that inadequate time resolution, and therefore insufficient compensation for these count losses, is one of the main reasons for size underestimation observed when analysing inorganic nanoparticles using ICP-MS, a topic still controversially discussed.</p>

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