Radioisotope X-Ray Fluorescence Spectrometry in Aquatic Biology: A Review
X-ray fluorescence spectrometry is described as an efficient means of carrying out non-destructive qualitative and quantitative chemical analyses of inorganic substances. A brief review is presented on the way in which this methodology was developed to recognize marked individuals and its application towards determining the geographic origin of organisms using multivariate analysis of the trace element composition of untagged animals. In addition, the results of experiments in which 34 species of invertebrates were compared and different parts of the same organisms irradiated, demonstrated that organisms may possess characteristic elemental compositions that are species specific. Multiple discriminant function analysis of the data showed that species may be classified on the basis of their chemical composition with few errors. Winter and summer growth of molluscan shells were distinguishable by the same method of elemental analysis, and a method for determining the age of organisms is given. Stepwise discriminant function analysis of the groups of elemental standards demonstrate that it is possible to isolate the most significant differences found between the groups compared by this methodology. The importance of these findings to studies in biogeochemistry, physiology, and pollution are briefly reviewed. X-ray fluorescence spectrometry is considered to be one of a number of related forms of data acquisition systems, each with broad applications.