Changes in Proximate Composition of the Crayfish Orconectes propinquus with Size, Sex, and Life Stage
We estimated percent water, inorganic material, and chitin for the crayfish Orconectes propinquus. To properly account for organic materials and mineral salts, we burned samples first at 550 C then at 650 C for estimates of the inorganic fraction. More precise estimates were obtained at 650 C; thus, to account for the conversion of CaCO3 to CaO and CO2 at 650 C, inorganic estimates were multiplied by an empirically derived factor (1.5) to convert all values to total inorganic material. For chitin, acid decalcification, followed by deproteinization with base and combustion at 650 C, provided best estimates. Proximate composition varied with size, sex, and molt stage. Percent water was inversely related to size for juveniles but was constant with size in adult males (68.4%) and females (67.4%). Percent inorganic material and chitin of dry weight increased with size in juveniles; inorganic continued to increase with size following maturity while percent chitin remained constant. For a carapace length of 25 mm, adult males had significantly more inorganic material (46.2 vs. 38.1%) and chitin (16.3 vs. 14.1%) than adult females. Differences in proximate composition appeared related to secondary sexual characteristics, with males having longer, wider chelae but narrower abdomens than females. Recent molts had more water (84.1%) and less inorganic (19.0%) and chitin (9.1%) than either male or female intermolts. Understanding how these proximates vary with life stage is necessary for adequately describing transfer of energy and biomass between populations.