Glomerular Mesangial Fibrosis in Hatchery-Reared Rainbow Trout (Salmo gairdneri)

1976 ◽  
Vol 33 (11) ◽  
pp. 2551-2559 ◽  
Author(s):  
David E. Hinton ◽  
Raymond T. Jones ◽  
Roger Lee Herman
Keyword(s):  
Rainbow Trout ◽  
Mesangial Cells ◽  
Salmo Gairdneri ◽  
Unknown Etiology ◽  
Electron Microscopic ◽  
Glomerular Lesion ◽  
Ultrastructural Studies ◽  
Glomerular Lesions ◽  
Microscopic Studies ◽  
Rate Comparison

Light and electron microscopic studies were performed on tissues of hatchery-reared rainbow trout (Salmo gairdneri) having a disease of currently unknown etiology with external symptoms of severe edema which causes increased mortality rate. Comparison with unaffected trout tissues revealed loss of cellularity in glomerular tufts with a replacement of mesangial cells by connective tissue. Ultrastructural studies showed the material to be collagen and established the diagnosis of glomerular mesangial fibrosis. The glomerular lesion was associated with alterations in lining epithelium of proximal tubules and extensive debris within tubule lumens. The findings are compared to glomerular lesions in mammalian and amphibian kidney.

Organized Intraglomerular Deposits in NZB Mouse Disease

1971 ◽  
Vol 29 ◽  
pp. 544-545
Author(s):  
Francis R. Comerford ◽  
Alan S. Cohen
Keyword(s):  
Systemic Lupus Erythematosus ◽  
Lupus Erythematosus ◽  
Antinuclear Antibodies ◽  
Human Systemic Lupus Erythematosus ◽  
Systemic Lupus ◽  
Glomerular Lesion ◽  
Ultrastructural Studies ◽  
Dense Deposits ◽  
Experimental Nephritis ◽  
Glomerular Lesions

Mice of the inbred NZB strain develop a spontaneous disease characterized by autoimmune hemolytic anemia, positive lupus erythematosus cell tests and antinuclear antibodies and nephritis. This disease is analogous to human systemic lupus erythematosus. In ultrastructural studies of the glomerular lesion in NZB mice, intraglomerular dense deposits in mesangial, subepithelial and subendothelial locations were described. In common with the findings in many examples of human and experimental nephritis, including many cases of human lupus nephritis, these deposits were amorphous or slightly granular in appearance with no definable substructure.We have recently observed structured deposits in the glomeruli of NZB mice. They were uncommon and were found in older animals with severe glomerular lesions by morphologic criteria. They were seen most commonly as extracellular elements in subendothelial and mesangial regions. The deposits ranged up to 3 microns in greatest dimension and were often adjacent to deposits of lipid-like round particles of 30 to 250 millimicrons in diameter and with amorphous dense deposits.

scholarly journals Improvements of glycol methacrylate. I. Its use as an embedding medium for electron microscopic studies.

1977 ◽  
Vol 25 (3) ◽  
pp. 163-174 ◽  
Author(s):  
R C Spaur ◽  
G C Moriarty
Keyword(s):  
Electron Beam ◽  
Water Soluble ◽  
Cross Linking ◽  
Electron Microscopic ◽  
Glycol Methacrylate ◽  
Ultrastructural Studies ◽  
Tissue Morphology ◽  
Methacrylate Monomer ◽  
Microscopic Studies ◽  
Embedding Medium

The technique for using the water-soluble embedding medium glycol methacrylate has been improved for ultrastructural studies by the simplification of the method of formation of prepolymers used in embedding the tissue, by the addition of a cross-linking agent so that sections are stable in the electron beam, and by improving the softness of the blocks by the addition of a plasticizing agent. The preservation of tissue morphology has been improved by complete dehydration in glycol methacrylate monomer prior to infiltration with the prepolymer. Preservations of tissue morphology is further enhanced by complete dehydration in ethanols and embedding in the improved glycol methacrylate medium.

Scanning and Transmission Electron Microscopic Observations of Rainbow Trout (Salmo gairdneri) Gill

1979 ◽  
Vol 36 (9) ◽  
pp. 1072-1079 ◽  
Author(s):  
Michael W. Kendall ◽  
James E. Dale
Keyword(s):  
Electron Microscopy ◽  
Rainbow Trout ◽  
Cellular Membrane ◽  
Salmo Gairdneri ◽  
Electron Microscopic ◽  
Variable Diameter ◽  
Physical Measurements ◽  
Transmission Electron ◽  
Secondary Lamellae ◽  
Gill Raker

The rainbow trout (Salmo gairdneri) gill was examined with scanning (SEM) and transmission (TEM) electron microscopic techniques as well as an improved glycol methacrylate embedding procedure for light microscopy. The gill epithelium on primary and secondary lamellae contained distinctive surface features consisting of whorled patterns of ridges (microfolds) with intervening grooves, measurements of which were correlated with measurements obtained from TEM tissue sections. The SEM and TEM data complimented each other concerning physical measurements and presence of lamellar ridges and grooves. The microfolds consisted of cellular membrane evaginations with furrows (grooves) between adjacent microfolds. The ridges and grooves on the primary lamellar surface were quite regular in their architecture whereas the ridges of secondary lamellae were farther apart and discontinuous. The gill raker surface also contained low ridges in a whorled pattern with wide intervening grooves. Small pores of variable diameter were observed on the secondary lamellar surface and much larger pores occurred on the primary lamellar and gill raker surface. TEM sections revealed the presence of chloride, pillar I and II, and epithelial cells and the fine structure of their relationship to the capillary bed. These observations were discussed with reference to their possible adaptive value to the trout. Key words: rainbow trout, branchial epithelium, primary lamella, secondary lamella, pillar cell, scanning electron microscopy, transmission electron microscopy

The renal glomerular lesions of pre-eclampsia: Electron microscopic studies

1963 ◽  
Vol 87 (8) ◽  
pp. 1047-1070 ◽  
Author(s):  
Conrad L. Pirani ◽  
Victor E. Pollak ◽  
Robert Lannigan ◽  
Giuseppe Folli
Keyword(s):  
Electron Microscopic ◽  
Glomerular Lesions ◽  
Microscopic Studies

Ultrastructure of Septa from the Filamentous Fungus Aspergillus Nidulans

1998 ◽  
Vol 4 (S2) ◽  
pp. 1142-1143
Author(s):  
Elizabeth A. Richardson ◽  
Michelle Momany
Keyword(s):  
Aspergillus Nidulans ◽  
Filamentous Fungus ◽  
Freeze Substitution ◽  
Uranyl Acetate ◽  
Temperature Sensitive ◽  
Electron Microscopic ◽  
Genetic Studies ◽  
Ultrastructural Studies ◽  
Microscopic Studies ◽  
Dialysis Membranes

The filamentous fungus Aspergillus nidulans partitions its cells by laying down septa at regularly spaced intervals in response to nuclear division. Physiological and genetic studies of the temperature-sensitive sep mutants have been especially useful in dissecting the regulation of septation. Electron microscopic studies of the sep mutants should be equally useful in dissecting the structural intermediates of septation. In preparation for ultrastructural studies of the sep mutants, we have examined septa in wild-type A. nidulans fixed by freeze substitution.Dialysis membranes were placed on rich medium plates and inoculated with A. nidulans spore suspensions. After 12 hours at 30°C, the dialysis membranes with adhering fungal hyphae were cut into square pieces measuring approximately 5mm on each side. The pieces were plunged into liquid propane and processed according to the procedures of Hoch. Serial sections were cut using a diamond knife and post stained with uranyl acetate and lead citrate.

Pulmonary Alveolar Microlithiasis: A Morphologic Study of Six Cases Including TEM and SEM with EDX Analysis and Immunocytochemistry

1990 ◽  
Vol 48 (2) ◽  
pp. 358-359
Author(s):  
S.R. Cole ◽  
L. Hochholzer ◽  
F.B. Johnson ◽  
D.R. Knibbs
Keyword(s):  
Fibrous Tissue ◽  
Armed Forces ◽  
Matrix Vesicles ◽  
Unknown Etiology ◽  
Electron Microscopic ◽  
Pulmonary Alveolar Microlithiasis ◽  
Transmission Electron ◽  
Microscopic Studies ◽  
Alveolar Microlithiasis ◽  
Phosphate Salts

Pulmonary alveolar microlithiasis (PAM) is a rare lung disease of unknown etiology characterized by calcified spherules which fill alveolar spaces. Although there are approximately 110 cases of PAM previously reported, only 26 have been confirmed by histologic examination. Increased interstitial fibrous tissue is seen in some cases, and some patients develop shortness of breath. However, many patients remain assymptomatic for years following the diagnosis of PAM.Six cases from the files of the Armed Forces Institute of Pathology and Hartford Hospital were studied. Light, scanning and transmission electron microscopic studies were performed as were chemical, immunocytochemical and energy dispersive x-ray analysis. Analyses suggest that they are composed in part of mucopolysaccharides and Ca3(PO4)2. Our findings indicate that microliths form initially around a cellular nidus by the deposition of calcium and phosphate salts from the extracellular matrix vesicles of surrounding cells similar to the method previously demonstrated 1n the formation of bone and psammoma bodies. PAM is not usually associated with Identifiable systemic disturbances in calcium and phosphorus metabolism.

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