scholarly journals ELECTRON MICROSCOPIC STUDIES ON THE MYOFIBRIL-LIKE FIBRILS IN THE MESANGIAL CELLS AND THE GLOMERULAR EPITHELIAL CELLS IN RAT RENAL CORTEX. (II)

1979 ◽  
Vol 43 (2) ◽  
pp. 125-135
Author(s):  
KAZUHIKO TANAKA ◽  
NOBUHIKO SHIBATA ◽  
NORIYUKI TATSUMI
Keyword(s):  
Epithelial Cells ◽  
Renal Cortex ◽  
Mesangial Cells ◽  
Electron Microscopic ◽  
Glomerular Epithelial Cells ◽  
Microscopic Studies

Mise en évidence du rôle, dans la régénération des Amphibiens, d'une glycoprotéine sécrétée par la cape apicale: étude cytochimique et autoradiographique en microscopie électronique

Development ◽  
1974 ◽  
Vol 32 (1) ◽  
pp. 133-145
Author(s):  
Par Claude Chapron
Keyword(s):  
High Resolution ◽  
Epithelial Cells ◽  
Limb Regeneration ◽  
Target Cells ◽  
Silver Particles ◽  
Electron Microscopic ◽  
Microscopic Studies ◽  
High Resolution Autoradiography

Evidence for the role of an apical cap glycoprotein in amphibian regeneration: cytochemical and autoradiographic electron-microscopic studies Early during limb regeneration in the newt, an ectodermal apical cap covering a mesodermal blastema is formed. High-resolution autoradiography of these tissues has been carried out after incorporation of [3H]fucose, which is a precursor of glycoproteins. Autoradiography shows that silver particles are located at first on epithelial cells, then on mesenchymatous cells. This observation is consistent with a hypothesis in which the apical cap would elaborate a glycoprotein acting on the blastema. Substructural autoradiography and cytochemistry also show the importance of cellular surfaces for both cells producing glycoprotein and those which are target cells.

scholarly journals Urokinase-plasminogen activator is synthesized in vitro by human glomerular epithelial cells but not by mesangial cells

1994 ◽  
Vol 45 (1) ◽  
pp. 43-47 ◽  
Author(s):  
Paul A.J. Brown ◽  
Heather M. Wilson ◽  
Fiona J. Reid ◽  
Nuala A. Booth ◽  
John G. Simpson ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Plasminogen Activator ◽  
Mesangial Cells ◽  
Urokinase Plasminogen Activator ◽  
Glomerular Epithelial Cells

scholarly journals ELECTRON MICROSCOPY OF THE BURSA OF FABRICIUS OF THE EMBRYONIC CHICK WITH PARTICULAR REFERENCE TO THE LYMPHO-EPITHELIAL NODULES

1962 ◽  
Vol 13 (1) ◽  
pp. 127-146 ◽  
Author(s):  
G. Adolph Ackerman
Keyword(s):  
Endoplasmic Reticulum ◽  
Epithelial Cells ◽  
Embryonic Development ◽  
Golgi Complex ◽  
Bursa Of Fabricius ◽  
Cortical Region ◽  
Electron Microscopic ◽  
Submicroscopic Structure ◽  
Microscopic Studies ◽  
Pass Through

Electron microscopic studies of the bursa of Fabricius during the 15th and 16th day of embryonic development in the chick have shown the following findings in the submicroscopic structure of the cellular elements of the lympho-epithelial follicles. In the medulla, basal endodermal epithelial cells undergo mitosis and differentiation into lymphoblasts. During this transformation, there is a reduction in the amount of rough endoplasmic reticulum, an increase in the number or ribosomes, and frequently an enlargement of the Golgi complex. As lymphoblasts differentiate into medium lymphocytes there is a loss of endoplasmic reticulum, a reduction in the number of ribosomes and in the size of the Golgi complex, as well as a decrease in the number and size of mitochondria and in the size of the cell and nucleus. Cytoplasmic processes of reticular-epithelial cells extend between proliferating lymphocytic cells. Desmosomes connect stellate reticular-epithelial and basal epithelial cells but are not present in lymphocytic cells. Nuclear blebbing and vesiculation are frequently observed in the various cell forms of the developing lympho-epithelial nodules. Although lymphocytes and lymphocytopoietic activities in the cortex are sparse during this stage of embryonic development of the bursa, transitional forms between mesenchymal cells and lymphoblasts have been encountered. In addition, lymphoblasts and/or undifferentiated epithelial cells occasionally may pass through the basement membrane from the medulla into the cortical region of the developing nodule. That lymphocytes in the bursa of Fabricius originate from both endodermal and mesodermal derivatives during embryonic development appears to be consistent with both light and electron microscopic observations.

Glomerular Mesangial Fibrosis in Hatchery-Reared Rainbow Trout (Salmo gairdneri)

1976 ◽  
Vol 33 (11) ◽  
pp. 2551-2559 ◽  
Author(s):  
David E. Hinton ◽  
Raymond T. Jones ◽  
Roger Lee Herman
Keyword(s):  
Rainbow Trout ◽  
Mesangial Cells ◽  
Salmo Gairdneri ◽  
Unknown Etiology ◽  
Electron Microscopic ◽  
Glomerular Lesion ◽  
Ultrastructural Studies ◽  
Glomerular Lesions ◽  
Microscopic Studies ◽  
Rate Comparison

Light and electron microscopic studies were performed on tissues of hatchery-reared rainbow trout (Salmo gairdneri) having a disease of currently unknown etiology with external symptoms of severe edema which causes increased mortality rate. Comparison with unaffected trout tissues revealed loss of cellularity in glomerular tufts with a replacement of mesangial cells by connective tissue. Ultrastructural studies showed the material to be collagen and established the diagnosis of glomerular mesangial fibrosis. The glomerular lesion was associated with alterations in lining epithelium of proximal tubules and extensive debris within tubule lumens. The findings are compared to glomerular lesions in mammalian and amphibian kidney.

scholarly journals Illustration of Pneumococcal Polysaccharide Capsule during Adherence and Invasion of Epithelial Cells

2005 ◽  
Vol 73 (8) ◽  
pp. 4653-4667 ◽  
Author(s):  
Sven Hammerschmidt ◽  
Sonja Wolff ◽  
Andreas Hocke ◽  
Simone Rosseau ◽  
Ellruth Müller ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Capsular Polysaccharide ◽  
A549 Cells ◽  
The State ◽  
Fixation Method ◽  
Fixation Procedure ◽  
Pneumococcal Infections ◽  
Electron Microscopic ◽  
Polysaccharide Capsule ◽  
Microscopic Studies

ABSTRACT The capsular polysaccharide of Streptococcus pneumoniae represents an important virulence factor and protects against phagocytosis. In this study the amount of capsular polysaccharide present on the bacterial surface during the infection process was illustrated by electron microscopic studies. After infection of A549 cells (type II pneumocytes) and HEp-2 epithelial cells a modified fixation method was used that allowed visualization of the state of capsule expression. This modified fixation procedure did not require the use of capsule-specific antibodies. Visualization of pneumococci in intimate contact and invading cells demonstrated that pneumococci were devoid of capsular polysaccharide. Pneumococci not in contact with the cells did not show alterations in capsular polysaccharide. After infection of the cells, invasive pneumococci of different strains and serotypes were recovered. Single colonies of these recovered pneumococci exhibited an up-to-105-fold-enhanced capacity to adhere and an up-to-104-fold-enhanced capacity to invade epithelial cells. Electron microscopic studies using a lysine-ruthenium red (LRR) fixation procedure or cryo-field emission scanning electron microscopy revealed a reduction in capsular material, as determined in detail for a serotype 3 pneumococcal strain. The amount of polysaccharide in the serotype 3 capsule was also determined after intranasal infection of mice. This study illustrates for the first time the phenotypic variation of the polysaccharide capsule in the initial phase of pneumococcal infections. The modified LRR fixation allowed monitoring of the state of capsule expression of pathogens during the infectious process.

Sign in / Sign up

Export Citation Format

Share Document