Methods and Feasibility of Using Aluminum-26 as a Biological Tracer in low pH Waters

1987 ◽  
Vol 44 (S1) ◽  
pp. s260-s263 ◽  
Author(s):  
Richard C. Playle
Keyword(s):  
Liquid Scintillation Counting ◽  
Liquid Scintillation ◽  
Low Ph ◽  
Scintillation Counting ◽  
Small Scale ◽  
Preliminary Results ◽  
Al Uptake

The fate of aluminum accumulated by fish can be studied using 26Al as a tracer, but the present cost of 26Al will keep experiments to a small scale and replication to a minimum. The methods developed for liquid scintillation counting of 26Al uptake will be most useful with exposures to low Al concentrations, where changes in Al concentration cannot be measured using other methods. Preliminary results showed that the gills and whole bodies of redbelly dace (Chrosomus eos Cope) accumulated more Al, when exposed to 480 μg∙L−1 Al at pH 5.1, than dace exposed to 200 μg∙L−1 Al at the same pH.

scholarly journals Biosynthesis of dermatan sulphate. Assay and properties of the uronosyl C-5 epimerase

1982 ◽  
Vol 201 (3) ◽  
pp. 489-493 ◽  
Author(s):  
A Malmström ◽  
L Aberg
Keyword(s):  
Enzyme Activity ◽  
Liquid Scintillation Counting ◽  
Liquid Scintillation ◽  
Divalent Cations ◽  
Heparan Sulphate ◽  
Low Ph ◽  
Scintillation Counting ◽  
Dermatan Sulphate ◽  
Ph Optimum ◽  
Cultured Fibroblasts

During biosynthesis of dermatan sulphate D-glucuronate (GlcA) residues are converted to L-iduronate (IdoA) residues via the reaction [Formula: see text]. The reaction occurs on the polymer level and is catalysed by a C-5 uronosyl epimerase. The reversible release of the C-5 hydrogen was utilized as a measure of the enzyme activity with 5-3H-labelled chondroitin as a substrate. 3H released during incubation was distilled and quantified by liquid-scintillation counting. The epimerase has a low pH optimum (5.6) and requires divalent cations, Mn2+ being the most efficient for activity. The Km for chondroitin is 1.2 × 10(-4) M. The epimerase is largely associated with the microsomal fractions (90%). Two-thirds of the activity can be solubilized by detergents. Microsomes from cultured fibroblasts contain two different uronosyl epimerases, one for the biosynthesis of heparan sulphate and one for that of dermatan sulphate. The two epimerases have different cofactor and pH requirements.

Light Filter for Reduction of Background in Liquid Scintillation Counting.

1964 ◽  
Vol 36 (11) ◽  
pp. 2080-2083 ◽  
Author(s):  
H. A. Swartz
Keyword(s):  
Liquid Scintillation Counting ◽  
Liquid Scintillation ◽  
Light Filter ◽  
Scintillation Counting

scholarly journals Determination of low-level biogenic gasoline, jet fuel, and diesel in blends using the direct liquid scintillation counting method for 14C content

Fuel ◽  
2021 ◽  
Vol 291 ◽  
pp. 120084
Author(s):  
Charles G. Doll ◽  
Andrew E. Plymale ◽  
Alan Cooper ◽  
Igor Kutnyakov ◽  
Marie Swita ◽  
...  
Keyword(s):  
Liquid Scintillation Counting ◽  
Liquid Scintillation ◽  
Jet Fuel ◽  
Scintillation Counting ◽  
Counting Method ◽  
Low Level
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