Identification of a major gene and RAPD markers for yellow seed coat colour in Brassica napus

Genome ◽  
2001 ◽  
Vol 44 (6) ◽  
pp. 1077-1082 ◽  
Author(s):  
Daryl J Somers ◽  
Gerhard Rakow ◽  
Vinod K Prabhu ◽  
Ken RD Friesen
Keyword(s):  
Brassica Napus ◽  
Seed Coat ◽  
Rapd Markers ◽  
Major Gene ◽  
Coat Colour ◽  
Seed Coat Colour ◽  
Yellow Seed ◽  
Dh Population ◽  
Seed Trait ◽  
Yellow Seed Coat

The development of yellow-seeded Brassica napus for improving the canola-meal quality characteristics of lower fibre content and higher protein content has been restricted because no yellow-seeded forms of B. napus exist, and their conventional development requires interspecific introgression of yellow seed coat colour genes from related species. A doubled-haploid (DH) population derived from the F1 generation of the cross 'Apollo' (black-seeded) × YN90-1016 (yellow-seeded) B. napus was analysed via bulked segregant analysis to identify molecular markers associated with the yellow-seed trait in B. napus for future implementation in marker-assisted breeding. A single major gene (pigment 1) flanked by eight RAPD markers was identified co-segregating with the yellow seed coat colour trait in the population. This gene explained over 72% of the phenotypic variation in seed coat colour. Further analysis of the yellow-seeded portion of this DH population revealed two additional genes favouring 'Apollo' alleles, explaining 11 and 8.5%, respectively, of the yellow seed coat colour variation. The data suggested that there is a dominant, epistatic interaction between the pigment 1 locus and the two additional genes. The potential of the markers to be implemented in plant breeding for the yellow-seed trait in B. napus is discussed.Key words: Brassica napus, yellow seed, RAPD.

Identification of a major gene and RAPD markers for yellow seed coat colour in Brassica napus

Genome ◽  
2001 ◽  
Vol 44 (6) ◽  
pp. 1077-1082 ◽  
Author(s):  
Daryl J. Somers ◽  
Gerhard Rakow ◽  
Vinod K. Prabhu ◽  
Ken R.D. Friesen
Keyword(s):  
Brassica Napus ◽  
Seed Coat ◽  
Rapd Markers ◽  
Major Gene ◽  
Coat Colour ◽  
Seed Coat Colour ◽  
Yellow Seed ◽  
Yellow Seed Coat

Verification of Soybean Seed Coat Colour Specific Markers Reveals I Loci Specific Markers Capable for Distinguishing Genotypes Differing in Seed Coated Colour

2020 ◽  
Author(s):  
R. B. Shingare ◽  
V. P. Chimote ◽  
M. P. Deshmukh ◽  
T. J. Bhor ◽  
A. A. Kale
Keyword(s):  
Seed Coat ◽  
Coat Color ◽  
Soybean Seed ◽  
Coat Colour ◽  
Specific Primers ◽  
Seed Coat Colour ◽  
Yellow Seed ◽  
Black Seed ◽  
Soybean Seed Coat ◽  
Yellow Seed Coat

Background: In soybean yellow seed coat is preferred in the market, however, colored ones are currently gaining attention because of their medicinal and nutritive values; besides. Hence it is essential to breed varieties with desired seed coat colour. Methods: Twelve genotypes with six each having yellow and black seed coats were screened with fourteen primers linked to seed coat colour governing loci. Result: Out of them twelve primers showed polymorphism. Monomorphism was observed with both T loci specific and two of the three R loci specific primers. However I locus specific primers i.e. SM303, SM305 and TR showed polymorphism shared by their seed coat color. SM303 amplified a 180 bp sized band in yellow seed coated genotypes and a 130 bp band in black seed coated genotypes. SM305 amplified dual bands with a 200bp band being monomorphic and an additional band (192-216 bp range) present in only yellow seed coated genotypes, of which a 208 bp band was shared by four yellow seed coated genotypes. Cold induced seed coat discoloration specific TR primer generated bands of different size ranges in yellow seed coated (336-344 bp) and black seed coated genotypes (300-320), of which a 340 bp band was shared by four yellow seed coated genotypes.

scholarly journals Targeted mutagenesis of BnTT8 homologs controls yellow seed coat development for effective oil production in Brassica napus L.

2020 ◽  
Vol 18 (5) ◽  
pp. 1153-1168 ◽  
Author(s):  
Yungu Zhai ◽  
Kaidi Yu ◽  
Shengli Cai ◽  
Limin Hu ◽  
Olalekan Amoo ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Seed Coat ◽  
Oil Production ◽  
Targeted Mutagenesis ◽  
Brassica Napus L ◽  
Yellow Seed ◽  
Yellow Seed Coat

Identification of QTL for seed coat colour and oil content in Brassica napus by association mapping using SSR markers

2015 ◽  
Vol 95 (2) ◽  
pp. 387-395 ◽  
Author(s):  
Cunmin Qu ◽  
Maen Hasan ◽  
Kun Lu ◽  
Liezhao Liu ◽  
Kai Zhang ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Association Mapping ◽  
Ssr Markers ◽  
Seed Coat ◽  
Oil Content ◽  
Coat Colour ◽  
Seed Coat Colour ◽  
Functional Polymorphisms ◽  
Genome Wide ◽  
Major Qtl

Qu, C., Hasan, M., Lu, K., Liu, L., Zhang, K., Fu, F., Wang, M., Liu, S., Bu, H., Wang, R., Xu, X., Chen, L. and Li, J. 2015. Identification of QTL for seed coat colour and oil content in Brassica napus by association mapping using SSR markers. Can. J. Plant Sci. 95: 387–395. Association mapping identifies quantitative trait loci (QTL) based on the strength of linkage disequilibrium (LD) between markers and functional polymorphisms across a set of diverse germplasms. In this study, we used association mapping to detect QTL and genome-wide simple sequence repeat (SSR) markers linked to seed coat colour and oil content in a population of 217 oilseed rape (Brassica napus L.) accessions. We corrected for the population structure of B. napus using 389 genome-wide SSR markers. In total, 25 and 11 SSR markers linked to seed coat colour and oil content were detected, respectively, and these two sets of markers were in different linkage groups. Nine of these markers for seed coat colour spanned the major QTL region for seed coat colour, and been mapped to chromosome A9. Six of these markers showed high levels of association with both seed coat colour and oil content, and markers H081N08.8 and KS20291 were mapped to the major QTL region for seed coat colour on chromosome A9. Another marker, CB10364, was in high LD with all determined seed coat colour and oil content traits, and was mapped to the co-localized QTL region for them on chromosome A8. These data indicate that seed coat colour was found to be an important contributor to seed oil content. Further, we show that association mapping using a heterogeneous set of genotypes is a suitable approach for complementing and enhancing previously obtained QTL information for marker-assisted selection.

Development of SCAR and CAPS Markers for a Partially Dominant Yellow Seed Coat Gene in Brassica Napus L

Euphytica ◽  
2006 ◽  
Vol 149 (3) ◽  
pp. 381-385 ◽  
Author(s):  
Zhiwen Liu ◽  
Tingdong Fu ◽  
Ying Wang ◽  
Jinxing Tu ◽  
Baoyuan Chen ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Seed Coat ◽  
Brassica Napus L ◽  
Yellow Seed ◽  
Caps Markers ◽  
Yellow Seed Coat

scholarly journals Embryonal Control of Yellow Seed Coat Locus ECY1 Is Related to Alanine and Phenylalanine Metabolism in the Seed Embryo of Brassica napus

2016 ◽  
Vol 6 (4) ◽  
pp. 1073-1081 ◽  
Author(s):  
Fulin Wang ◽  
Jiewang He ◽  
Jianghua Shi ◽  
Tao Zheng ◽  
Fei Xu ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Seed Coat ◽  
Yellow Seed ◽  
Seed Embryo ◽  
Phenylalanine Metabolism ◽  
Yellow Seed Coat

Correlation Studies of Some Agronomic Characters in Sesame

1970 ◽  
Vol 6 (1) ◽  
pp. 27-31 ◽  
Author(s):  
M. Osman Khidir ◽  
H. El Gizouli Osman
Keyword(s):  
Seed Size ◽  
Seed Coat ◽  
Coat Colour ◽  
Stem Height ◽  
Agronomic Characters ◽  
Seed Coat Colour ◽  
Correlation Studies ◽  
First Maturity ◽  
Criteria For Selection ◽  
Number Of Branches

SummaryIn 90 local sesame types there was some association between seed coat colour and seed size, stem height, number of branches, number of pods, yield per plant and earliness. Forty-five coefficients show the degree of correlation between ten agronomic characters. Yield was significantly and positively correlated with all characters except the number of days to first flowering and to first maturity. Stem height, number of pods per plant and seed size seem to be the best criteria for selection in sesame.

scholarly journals Mapping and identification of yellow seed coat color genes in Brassica juncea L.

2020 ◽  
Author(s):  
Zhen Huang ◽  
Yang Wang ◽  
Hong Lu ◽  
Xiang Liu ◽  
Lu Liu ◽  
...  
Keyword(s):  
Candidate Gene ◽  
Brassica Juncea ◽  
Seed Coat ◽  
Coat Color ◽  
Seed Color ◽  
Seed Coat Color ◽  
Flavonoid Pathway ◽  
Yellow Seed ◽  
Color Formation ◽  
Yellow Seed Coat

Abstract BackgroundYellow seed breeding is an effective method to improve the oil content in rapeseed. Yellow seed coat color formation is influenced by various factors, and no clear mechanisms are known. In this study, Bulked segregant RNA-Seq (BSR-Seq) of BC9 population of Wuqi mustard (yellow seed) and Wugong mustard (brown seed) was used to identity the candidate genes controlling the yellow seed color in Brassica juncea L.ResultsYellow seed coat color gene was mapped to chromosome A09, and differentially expressed genes (DEGs) between brown and yellow bulks enriched in the flavonoid pathway. A significant correlation between the expression of BjF3H and BjTT5 and the content of the seed coat color related indexes was identified. Two intron polymorphism (IP) markers linked to the target gene were developed around BjF3H. Therefore, BjF3H was considered as the candidate gene. The BjF3H coding sequences (CDS) of Wuqi mustard and Wugong mustard are 1071-1077bp, encoding protein of 356-358 amino acids. One amino acid change (254, F/V) was identified in the conserved domain. This mutation site was detected in four Brassica rapa (B. rapa) and six Brassica juncea (B. juncea) lines, but not in Brassica napus (B. napus).ConclusionsThe results indicated BjF3H is a candidate gene that related to yellow seed coat color formation in Brassica juncea and provided a comprehensive understanding of the yellow seed coat color mechanism.

Genotypic variation for phenolic compounds in developing and whole seeds, and storage conditions influence visual seed quality of yellow dry bean genotypes

2020 ◽  
Vol 100 (3) ◽  
pp. 284-295
Author(s):  
Mei Xiong ◽  
Mengli Zhao ◽  
Zhen-Xiang Lu ◽  
Parthiba Balasubramanian
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Seed Coat ◽  
Storage Temperature ◽  
Condensed Tannin ◽  
Consumer Preference ◽  
Coat Colour ◽  
Seed Coat Colour ◽  
Duration Of Storage

Seed coat colour is an important determinant of the visual quality of dry beans, as seeds are sold as a dry commodity. Phenolic compounds have a major effect on the colour of bean seeds. The objectives of the study were to determine the changes in phenolic compounds during seed development and in whole seeds of yellow bean genotypes with contrasting seed coat colour, and the effects of storage temperature and duration on seed phenolics and colour. Condensed tannin, phenolic acid, flavonoids, and antioxidant activity were observed as early as 10 d after flowering in the developing seeds of Arikara Yellow, which darken at harvest and during postharvest storage. In contrast, for CDC Sol and AAC Y073 seeds which remain yellow, phenolic compounds and antioxidant activity were consistently low. Seed brightness (L*) and yellow colour (b*) were negatively correlated with phenolic compounds and antioxidant activity, and conversely seed redness (a*) was positively correlated with phenolic compounds, confirming a negative influence of phenolic compounds on seed coat colour. Yellow bean genotypes had low anthocyanin but were high in β-carotene. Storage temperature influenced condensed tannin and seed coat colour, whereas the duration of storage influenced phenolic compounds, antioxidant activity, and seed coat colour. Higher temperatures (20 or 30 °C) and longer storage duration (120 or 180 d) generally resulted in darker seeds with increasing redness compared with seeds stored at 6 °C or for 60 d. AAC Y073 and CDC Sol with improved seed coat colour may increase consumer preference, value, and marketability of yellow beans.

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