A resistance-like gene identified by EST mapping and its association with a QTL controlling Fusarium head blight infection on wheat chromosome 3BS

Genome ◽  
2006 ◽  
Vol 49 (6) ◽  
pp. 631-635 ◽  
Author(s):  
Xiaorong Shen ◽  
Michael G Francki ◽  
Herbert W Ohm
Keyword(s):  
Disease Resistance ◽  
Fusarium Head Blight ◽  
Resistance Genes ◽  
Expressed Sequence Tags ◽  
Quantitative Trait ◽  
Disease Resistance Genes ◽  
Head Blight ◽  
Fhb Resistance ◽  
Expressed Sequence ◽  
Chromosome 3B

Fusarium head blight (FHB) is a major disease in the wheat growing regions of the world. A quantitative trait locus (QTL) on the short arm of chromosome 3B controls much of the variation for resistance. The cloning of candidate disease-resistance genes for FHB QTLs on chromosome 3B can provide further elucidation of the mechanisms that control resistance. However, rearrangements and divergence during plant genome evolution often hampers the identification of sequences with similarity to known disease-resistance genes. This study focuses on the use of wheat expressed sequence tags (ESTs) that map to the region on chromosome 3B containing the QTL for FHB resistance and low-stringency BLAST searching to identify sequences with similarity to known disease-resistance genes. One EST rich with leucine repeats and low similarity to a protein kinase domain of the barley Rpg1 gene was identified. Genetic mapping using a Ning894037 × Alondra recombinant inbred (RI) population showed that this EST mapped to the QTL on the short arm of chromosome 3B and may represent a portion of a newly diverged gene contributing to FHB resistance. The EST is a new marker suitable for marker-assisted selection and provides a starting point to begin map-based cloning for chromosome walking and investigate new diverged genes at this locus.Key words: Fusarium head blight resistance, expressed sequence tags, quantitative trait loci, Rpg1, wheat.

scholarly journals The Ability to Detoxify the Mycotoxin Deoxynivalenol Colocalizes With a Major Quantitative Trait Locus for Fusarium Head Blight Resistance in Wheat

2005 ◽  
Vol 18 (12) ◽  
pp. 1318-1324 ◽  
Author(s):  
Marc Lemmens ◽  
Uwe Scholz ◽  
Franz Berthiller ◽  
Chiara Dall'Asta ◽  
Andrea Koutnik ◽  
...  
Keyword(s):  
Quantitative Trait Locus ◽  
Fusarium Head Blight ◽  
Quantitative Trait ◽  
Fusarium Head Blight Resistance ◽  
Close Relation ◽  
Major Effect ◽  
Head Blight ◽  
Fhb Resistance ◽  
Trait Locus ◽  
Wheat Lines

We investigated the hypothesis that resistance to deoxynivalenol (DON) is a major resistance factor in the Fusarium head blight (FHB) resistance complex of wheat. Ninety-six double haploid lines from a cross between ‘CM-82036’ and ‘Remus’ were examined. The lines were tested for DON resistance after application of the toxin in the ear, and for resistances to initial infection and spread of FHB after artificial inoculation with Fusarium spp. Toxin application to flowering ears induced typical FHB symptoms. Quantitative trait locus (QTL) analyses detected one locus with a major effect on DON resistance (logarithm of odds = 53.1, R2 = 92.6). The DON resistance phenotype was closely associated with an important FHB resistance QTL, Qfhs.ndsu-3BS, which previously was identified as governing resistance to spread of symptoms in the ear. Resistance to the toxin was correlated with resistance to spread of FHB (r = 0.74, P < 0.001). In resistant wheat lines, the applied toxin was converted to DON-3-O-glucoside as the detoxification product. There was a close relation between the DON-3-glucoside/DON ratio and DON resistance in the toxintreated ears (R2 = 0.84). We conclude that resistance to DON is important in the FHB resistance complex and hypothesize that Qfhs.ndsu-3BS either encodes a DON-glucosyltransferase or regulates the expression of such an enzyme.

scholarly journals Reevaluation of Two Quantitative Trait Loci for Type II Resistance to Fusarium Head Blight in Wheat Germplasm PI 672538

2017 ◽  
Vol 107 (1) ◽  
pp. 92-99 ◽  
Author(s):  
X. Li ◽  
Z. P. Xiang ◽  
W. Q. Chen ◽  
Q. L. Huang ◽  
T. G. Liu ◽  
...  
Keyword(s):  
Quantitative Trait Loci ◽  
Fusarium Head Blight ◽  
Phenotypic Variation ◽  
Quantitative Trait ◽  
Head Blight ◽  
Resistance Response ◽  
Major Qtls ◽  
Trait Loci ◽  
Fhb Resistance ◽  
Resistance Data

Fusarium head blight (FHB), mainly caused by Fusarium graminearum, is a destructive disease in wheat. A population consisting of 229 F2 and F2:3 plants derived from the cross PI 672538 × L661 was used to evaluate the reactions to FHB. The FHB resistance data distribution in the F2 population indicates that some quantitative trait loci (QTLs) were controlling the FHB resistance in PI 672538. We further detected two major QTLs (Qfhs-2B, Qfhs-3B) from analysis of the resistance data and the PCR-amplified results using WinQTLCart 2.5 software. Qfhs-2B, flanked by Xbarc55-2B and Xbarc1155-2B, explained more than 11.6% of the phenotypic variation of the percentage of diseased spikelets (PDS), and Qfhs-3B, flanked by Xwmc54-3B and Xgwm566-3B, explained more than 10% of the PDS phenotypic variation in the F2:3 population. In addition, Qfhs-3B was different from Fhb1 in terms of the pedigree, inheritance, resistance response, chromosomal location, and marker diagnosis. We also detected QTLs for other disease resistance indices, including the percentage of damaged kernels and 1,000-grain weight, in similar chromosomal regions. Therefore, the FHB resistance of PI 672538 was mainly controlled by two major QTLs, mapped on 2B (FhbL693a) and 3B (FhbL693b). PI 672538 could be a useful germplasm for improving wheat FHB resistance.

scholarly journals Wheat resistance to Fusarium head blight and possibilities of its improvement using molecular marker-assisted selection

2017 ◽  
Vol 53 (No. 2) ◽  
pp. 47-54 ◽  
Author(s):  
L. Shah ◽  
A. Ali ◽  
Y. Zhu ◽  
S. Wang ◽  
H. Si ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Fusarium Head Blight ◽  
Resistance Genes ◽  
Marker Assisted Selection ◽  
Gene Pyramiding ◽  
Head Blight ◽  
Crop Breeding ◽  
Resistance Qtls ◽  
Fhb Resistance ◽  
Or Genes

Wheat, one of the world’s major crops, is seriously affected by fungal diseases, especially in regions with high moisture and moderately warm temperatures. This paper reviews various molecular and conventional techniques that are used to identify genotypes with resistance alleles associated with Fusarium head blight (FHB) diseases. Quantitative trait loci (QTL) type II, designated as Fhb1, are frequently applied in plant breeding, and the newly recognized genes related to resistance to this fungal disease give extra insights into marker-assisted selection (MAS). Molecular markers are robust tools that may be routinely used in MAS for the mapping of resistance genes in crop breeding. FHB resistance is polygenic, and different resistance genes could be conveyed into a single genotype by MAS, which might ensure greater resistance to FHB disease. In conclusion, different researchers have used various techniques to control FHB resistance, such as MAS, gene pyramiding (through backcross), and molecular markers (association with resistance QTLs or genes).  

scholarly journals Quantitative Trait Locus Mapping of Increased Fusarium Head Blight Susceptibility Associated with a Wild Emmer Wheat Chromosome

2009 ◽  
Vol 99 (4) ◽  
pp. 447-452 ◽  
Author(s):  
David F. Garvin ◽  
Robert W. Stack ◽  
Jana M. Hansen
Keyword(s):  
Quantitative Trait Locus ◽  
Fusarium Head Blight ◽  
Quantitative Trait ◽  
Tetraploid Wheat ◽  
Wild Emmer Wheat ◽  
Emmer Wheat ◽  
Wild Emmer ◽  
Head Blight ◽  
Fhb Resistance ◽  
Trait Locus

Chromosome 2A of wild emmer wheat (Triticum turgidum var. dicoccoides) genotype Israel A increases Fusarium head blight (FHB) severity when present in durum wheat (T. turgidum var. durum) cv. Langdon (LDN). The goal of this study was to identify regions of Israel A chromosome 2A associated with this difference in resistance. A recombinant inbred chromosome line population (RICL) from a cross between LDN and the LDN-Israel A chromosome 2A substitution line [LDN(DIC-2A)] was employed for analysis. Three greenhouse FHB evaluations were completed on the RICL to obtain phenotypic data on variation for FHB resistance, and a simple sequence repeat (SSR)-based molecular map of chromosome 2A was developed. Quantitative trait locus (QTL) mapping identified a region on the long arm of chromosome 2A that was associated with FHB resistance in each independent FHB evaluation. Depending on the evaluation, the single best SSR marker in this region accounted for between 21 and 26% of the variation for FHB resistance, with the Israel A marker alleles associated with increased FHB susceptibility. The single best markers from each evaluation reside within an interval of approximately 22 cM. This study identifies one or more new QTL on chromosome 2A in tetraploid wheat that can contribute to significant variation in FHB resistance.

scholarly journals Identification of a Novel Fusarium Head Blight Resistance Quantitative Trait Locus on Chromosome 7A in Tetraploid Wheat

2007 ◽  
Vol 97 (5) ◽  
pp. 592-597 ◽  
Author(s):  
S. Kumar ◽  
R. W. Stack ◽  
T. L. Friesen ◽  
J. D. Faris
Keyword(s):  
Quantitative Trait Locus ◽  
Fusarium Head Blight ◽  
Quantitative Trait ◽  
Recombinant Inbred Lines ◽  
Tetraploid Wheat ◽  
Fusarium Head Blight Resistance ◽  
Resistance Quantitative Trait Locus ◽  
Head Blight ◽  
Fhb Resistance ◽  
Trait Locus

Fusarium head blight (FHB) caused by Fusarium graminearum is one of the most destructive diseases of durum (Triticum turgidum sp. durum) and common wheat (T. aestivum). Promising sources of FHB resistance have been identified among common (hexaploid) wheats, but the same is not true for durum (tetraploid) wheats. A previous study indicated that chromosome 7A from T. turgidum sp. dicoccoides accession PI478742 contributed significant levels of resistance to FHB. The objectives of this research were to develop a genetic linkage map of chromosome 7A in a population of 118 recombinant inbred lines derived from a cross between the durum cv. Langdon (LDN) and a disomic LDN-T. turgidum sp. dicoccoides PI478742 chromosome 7A substitution line [LDN-DIC 7A(742)], and identify a putative FHB resistance quantitative trait locus (QTL) on chromosome 7A derived from LDN-DIC 7A(742). The population was evaluated for type II FHB resistance in three greenhouse environments. Interval regression analysis indicated that a single QTL designated Qfhs.fcu-7AL explained 19% of the phenotypic variation and spanned an interval of 39.6 cM. Comparisons between the genetic map and a previously constructed physical map of chromosome 7A indicated that Qfhs.fcu-7AL is located in the proximal region of the long arm. This is only the second FHB QTL to be identified in a tetraploid source, and it may be useful to combine it with the QTL Qfhs.ndsu-3AS in order to develop durum wheat germ plasm and cultivars with higher levels of FHB resistance.

scholarly journals Identification of Functional Genic Components of Major Fusarium Head Blight Resistance Quantitative Trait Loci in Wheat Cultivar Sumai 3

2013 ◽  
Vol 26 (4) ◽  
pp. 442-450 ◽  
Author(s):  
Yongbin Zhuang ◽  
Aravind Gala ◽  
Yang Yen
Keyword(s):  
Quantitative Trait Loci ◽  
Fusarium Head Blight ◽  
Quantitative Trait ◽  
Defense Mechanism ◽  
Fusarium Head Blight Resistance ◽  
Head Blight ◽  
Bulk Analysis ◽  
Trait Loci ◽  
Fhb Resistance ◽  
Sumai 3

Fusarium head blight (FHB) is a devastating disease worldwide, affecting wheat and other small grains. To identify key wheat genes involved in FHB pathogenesis, 406 FHB-related wheat expressed sequence tags functionally identified in Sumai 3 were investigated for their association with FHB-resistance quantitative trait loci (QTL) Fhb1 and Fhb_6BL in 2010 and 2011. A total of 47 candidate genes were identified by bulk analysis, near-isogenic screening and expression QTL mapping, and were finally mapped to their carrier chromosomes with Chinese Spring nulli-tetra deficiency lines. One gene, designated WFhb1_c1 (wheat Fhb1 candidate gene 1), was both functionally associated with and physically located within Fhb1 and was found to be weakly similar (E = 5e+0) to an Arabidopsis gene encoding pectin methyl esterase inhibitor. Two other genes, designated WFI_6BL1 and WFI_6BL2 (wheat-Fusarium interaction genes 6BL1 and 6BL2), were functionally associated with Fhb_6BL but physically mapped on chromosomes 7D and 5A, respectively. WFI_6BL1 was annotated as a 13- lipoxygenase gene and WFI_6BL2 might encode a PR-4-like protein. Our data suggested that i) Fhb1 seems to contribute to FHB resistance by reducing susceptibility in the first 60 h, ii) Fhb_6BL makes its contribution via the jasmonate-mediated pathways, and iii) wheat seems to activate its defense mechanism in the biotrophic phase of FHB pathogenesis.

scholarly journals Pyramiding of Fusarium Head Blight Resistance Quantitative Trait Loci, Fhb1, Fhb4, and Fhb5, in Modern Chinese Wheat Cultivars

2021 ◽  
Vol 12 ◽  
Author(s):  
Yiduo Zhang ◽  
Zibo Yang ◽  
Haicai Ma ◽  
Liying Huang ◽  
Feng Ding ◽  
...  
Keyword(s):  
Fusarium Head Blight ◽  
Quantitative Trait ◽  
Disease Spread ◽  
Resistance Quantitative Trait Locus ◽  
Wheat Cultivars ◽  
Head Blight ◽  
Effective Measure ◽  
Modern Chinese ◽  
Fhb Resistance ◽  
Chinese Wheat

Wheat production is increasingly threatened by the fungal disease, Fusarium head blight (FHB), caused by Fusarium spp. The introduction of resistant varieties is considered to be an effective measure for containment of this disease. Mapping of FHB-resistance quantitative trait locus (QTL) has promoted marker-assisted breeding for FHB resistance, which has been difficult through traditional breeding due to paucity of resistance genes and quantitative nature of the resistance. The lab of Ma previously cloned Fhb1, which inhibits FHB spread within spikes, and fine mapped Fhb4 and Fhb5, which condition resistance to initial infection of Fusarium spp., from FHB-resistant indigenous line Wangshuibai (WSB). In this study, these three QTLs were simultaneously introduced into five modern Chinese wheat cultivars or lines with different ecological adaptations through marker-assisted backcross in early generations. A total of 14 introgression lines were obtained. All these lines showed significantly improved resistance to the fungal infection and disease spread in 2-year field trials after artificial inoculation. In comparison with the respective recipient lines, the Fhb1, Fhb4, and Fhb5 pyramiding could reduce the disease severity by 95% and did not systematically affect plant height, productive tiller number, kernel number per spike, thousand grain weight, flowering time, and unit yield (without Fusarium inoculation). These results indicated the great value of FHB-resistance QTLs Fhb1, Fhb4, and Fhb5 derived from WSB, and the feasibility and effectiveness of early generation selection for FHB resistance solely based on linked molecular markers.

scholarly journals Mapping of Quantitative Trait Loci for Fusarium Head Blight Resistance in Barley

2000 ◽  
Vol 90 (10) ◽  
pp. 1079-1088 ◽  
Author(s):  
Zhengqiang Ma ◽  
Brian J. Steffenson ◽  
Louis K. Prom ◽  
Nora L. V. Lapitan
Keyword(s):  
Quantitative Trait Loci ◽  
Fusarium Head Blight ◽  
Plant Height ◽  
Quantitative Trait ◽  
Morphological Traits ◽  
Heading Date ◽  
Rflp Markers ◽  
Head Blight ◽  
Trait Loci ◽  
Fhb Resistance

Fusarium head blight (FHB) is a devastating disease that causes significant reductions in yield and quality in wheat and barley. Barley grains infected with deoxynivalenol (DON), a vomitoxin produced by Fusarium graminearum, are rejected for malting and brewing. Among six-rowed barley cultivars tested thus far, only cv. Chevron exhibited resistance. This study was conducted to map genes and to identify DNA markers for marker-assisted breeding for FHB resistance in cv. Chevron with restriction fragment length polymorphism (RFLP) markers. A doubled haploid (DH) population was created from a cross between cv. Chevron and susceptible cv. Stander. Seven field experiments were conducted in four different locations in 2 years. A RFLP map containing 211 loci and covering over 1,000 centimorgans (cM) of the genome was used to map quantitative trait loci (QTL) associated with relatively low FHB severity and DON concentration. Morphological traits differing between the parents were also measured: heading date, plant height, spike angle, number of nodes per cm of rachis in the spike, and kernel plumpness. Many of the QTL for FHB and DON coincided with QTLs for these morphological traits. The “fix-QTL” algorithm in Mapmaker QTL was used to remove the part of the variance for FHB resistance that may be explained by heading date or plant height. Results from this study suggest that QTLs with major effects for FHB resistance probably do not exist in cv. Chevron. Three QTL intervals, Xcmwg706-Xbcd441 on chromosome 1H, Xbcd307b-Xcdo684b on chromosome 2H, and Xcdo959b-Xabg472 on chromosome 4H, that are not associated with late heading or height may be useful for marker-assisted selection.

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