scholarly journals Wheat resistance to Fusarium head blight and possibilities of its improvement using molecular marker-assisted selection

2017 ◽  
Vol 53 (No. 2) ◽  
pp. 47-54 ◽  
Author(s):  
L. Shah ◽  
A. Ali ◽  
Y. Zhu ◽  
S. Wang ◽  
H. Si ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Fusarium Head Blight ◽  
Resistance Genes ◽  
Marker Assisted Selection ◽  
Gene Pyramiding ◽  
Head Blight ◽  
Crop Breeding ◽  
Resistance Qtls ◽  
Fhb Resistance ◽  
Or Genes

Wheat, one of the world’s major crops, is seriously affected by fungal diseases, especially in regions with high moisture and moderately warm temperatures. This paper reviews various molecular and conventional techniques that are used to identify genotypes with resistance alleles associated with Fusarium head blight (FHB) diseases. Quantitative trait loci (QTL) type II, designated as Fhb1, are frequently applied in plant breeding, and the newly recognized genes related to resistance to this fungal disease give extra insights into marker-assisted selection (MAS). Molecular markers are robust tools that may be routinely used in MAS for the mapping of resistance genes in crop breeding. FHB resistance is polygenic, and different resistance genes could be conveyed into a single genotype by MAS, which might ensure greater resistance to FHB disease. In conclusion, different researchers have used various techniques to control FHB resistance, such as MAS, gene pyramiding (through backcross), and molecular markers (association with resistance QTLs or genes).  

scholarly journals Characterisation of fusarium head blight resistance located on chromosome 4A of Triticum macha

2017 ◽  
Vol 38 (SI 2 - 6th Conf EFPP 2002) ◽  
pp. 580-582 ◽  
Author(s):  
A. Steed ◽  
E. Chandler ◽  
M. Thomsett ◽  
J. Carter ◽  
S. Faure ◽  
...  
Keyword(s):  
Fusarium Head Blight ◽  
Marker Assisted Selection ◽  
Single Gene ◽  
Fusarium Head Blight Resistance ◽  
Type I ◽  
Head Blight ◽  
Yield Trait ◽  
Fhb Resistance ◽  
Wheat Lines ◽  
Triticum Macha

Chromosome 4A of Triticum macha carries resistance to Fusarium head blight (FHB). Double haploid lines (DH) of T. macha 4A were used to determine the type of resistance and location of the gene(s). FHB resistance and yield trait data collected over two seasons following spray and point inoculation, indicate that the resistance is of type I and is probably conferred by a single gene. The resistance was mapped with microsatellite markers to a small area of the T. macha 4A chromosome flanked by markers gwm 610 and gwm 165. This could greatly facilitate future marker assisted selection work aimed at increasing resistance to FHB in other winter wheat lines.

DNA marker analysis for pyramided of Fusarium head blight (FHB) resistance QTLs from different germplasm

Genetica ◽  
2007 ◽  
Vol 133 (1) ◽  
pp. 77-84 ◽  
Author(s):  
J. R. Shi ◽  
D. H. Xu ◽  
H. Y. Yang ◽  
Q. X. Lu ◽  
T. Ban
Keyword(s):  
Fusarium Head Blight ◽  
Dna Marker ◽  
Head Blight ◽  
Resistance Qtls ◽  
Marker Analysis ◽  
Fhb Resistance

scholarly journals A spontaneous segmental deletion from chromosome arm 3DL enhances Fusarium head blight resistance in wheat

Genome ◽  
2015 ◽  
Vol 58 (11) ◽  
pp. 479-488 ◽  
Author(s):  
David F. Garvin ◽  
Hedera Porter ◽  
Zachary J. Blankenheim ◽  
Shiaoman Chao ◽  
Ruth Dill-Macky
Keyword(s):  
Molecular Markers ◽  
Fusarium Head Blight ◽  
Molecular Mapping ◽  
Wheat Cultivar ◽  
Fusarium Head Blight Resistance ◽  
Disease Spread ◽  
Resistance Locus ◽  
Head Blight ◽  
Chromosome Arm ◽  
Fhb Resistance

Much effort has been directed at identifying sources of resistance to Fusarium head blight (FHB) in wheat. We sought to identify molecular markers for what we hypothesized was a new major FHB resistance locus originating from the wheat cultivar ‘Freedom’ and introgressed into the susceptible wheat cultivar ‘USU-Apogee’. An F2:3 mapping population from a cross between Apogee and A30, its BC4 near-isoline exhibiting improved FHB resistance, was evaluated for resistance. The distribution of FHB resistance in the population approximated a 1:3 moderately resistant : moderately susceptible + susceptible ratio. Separate disease evaluations established that A30 accumulated less deoxynivalenol and yielded a greater proportion of sound grain than Apogee. Molecular mapping revealed that the FHB resistance of A30 is associated with molecular markers on chromosome arm 3DL that exhibit a null phenotype in A30 but are present in both Apogee and Freedom, indicating a spontaneous deletion occurred during the development of A30. Aneuploid analysis revealed that the size of the deleted segment is approximately 19% of the arm’s length. Our results suggest that the deleted interval of chromosome arm 3DL in Apogee may harbor FHB susceptibility genes that promote disease spread in infected spikes, and that their elimination increases FHB resistance in a novel manner.

The effect of 3BS locus of Sumai 3 on Fusarium head blight resistance in Australian wheats

2007 ◽  
Vol 47 (5) ◽  
pp. 603 ◽  
Author(s):  
G. Q. Xie ◽  
M. C. Zhang ◽  
S. Chakraborty ◽  
C. J. Liu
Keyword(s):  
Molecular Markers ◽  
Fusarium Head Blight ◽  
Fusarium Head Blight Resistance ◽  
Wheat Breeding ◽  
Head Blight ◽  
Breeding Programs ◽  
Backcross Populations ◽  
Australian Wheat ◽  
Fhb Resistance ◽  
Sumai 3

The 3BS allele of Sumai 3 has been the main source of Fusarium head blight (FHB) resistance worldwide. Using molecular markers and FHB resistance screenings, we have analysed the effects of this allele in two backcross and two 4-way F2 populations derived from elite Australian cultivars. Compared to individuals without the Sumai 3 allele, individuals with the allele showed an average 32.0% reduction in FHB severity as measured by number of diseased spikelets. This value was slightly reduced to 29.2% when the total number of spikelets was taken into account by expressing severity as the proportion of diseased spikelets. When compared to the parental cultivars, progeny with the 3BS allele of Sumai 3 offered, on average, 43.3% reduction in FHB severity. Significant differences were not detected between progeny that were homozygous or heterozygous for the 3BS locus, indicating a dominant inheritance of this locus. These results confirm that the 3BS allele controls a large component of the FHB resistance in Sumai 3, which can be readily incorporated and detected in backcross populations using molecular markers. The materials derived from this study could offer significant benefits to the Australian wheat breeding programs.

A resistance-like gene identified by EST mapping and its association with a QTL controlling Fusarium head blight infection on wheat chromosome 3BS

Genome ◽  
2006 ◽  
Vol 49 (6) ◽  
pp. 631-635 ◽  
Author(s):  
Xiaorong Shen ◽  
Michael G Francki ◽  
Herbert W Ohm
Keyword(s):  
Disease Resistance ◽  
Fusarium Head Blight ◽  
Resistance Genes ◽  
Expressed Sequence Tags ◽  
Quantitative Trait ◽  
Disease Resistance Genes ◽  
Head Blight ◽  
Fhb Resistance ◽  
Expressed Sequence ◽  
Chromosome 3B

Fusarium head blight (FHB) is a major disease in the wheat growing regions of the world. A quantitative trait locus (QTL) on the short arm of chromosome 3B controls much of the variation for resistance. The cloning of candidate disease-resistance genes for FHB QTLs on chromosome 3B can provide further elucidation of the mechanisms that control resistance. However, rearrangements and divergence during plant genome evolution often hampers the identification of sequences with similarity to known disease-resistance genes. This study focuses on the use of wheat expressed sequence tags (ESTs) that map to the region on chromosome 3B containing the QTL for FHB resistance and low-stringency BLAST searching to identify sequences with similarity to known disease-resistance genes. One EST rich with leucine repeats and low similarity to a protein kinase domain of the barley Rpg1 gene was identified. Genetic mapping using a Ning894037 × Alondra recombinant inbred (RI) population showed that this EST mapped to the QTL on the short arm of chromosome 3B and may represent a portion of a newly diverged gene contributing to FHB resistance. The EST is a new marker suitable for marker-assisted selection and provides a starting point to begin map-based cloning for chromosome walking and investigate new diverged genes at this locus.Key words: Fusarium head blight resistance, expressed sequence tags, quantitative trait loci, Rpg1, wheat.

Advances in genetic improvement of durable resistance to Fusarium head blight in wheat

2021 ◽  
pp. 191-242
Author(s):  
Guihua Bai ◽  
Keyword(s):  
Fusarium Head Blight ◽  
Marker Assisted Selection ◽  
Genetic Improvement ◽  
Durable Resistance ◽  
Phenotypic Selection ◽  
Reference Sequence ◽  
Type Ii ◽  
Head Blight ◽  
Fhb Resistance ◽  
Different Sources

Wheat Fusarium head blight (FHB) is a destructive disease in wheat worldwide. Wheat resistance to FHB is a complex with five types. Each type of resistance is controlled by multiple quantitative trait loci (QTLs) with most having minor effects and being affected by environments. This chapter describes methodologies used for evaluating different types of resistance, consolidates the QTLs for type II and Type III resistance into 26 repeatable QTLs, discusses progresses made in genetics and breeding of wheat FHB resistance, and discusses possible new breeding strategies for FHB resistance improvement. The 26 repeatable QTL were located in ~100 Mb intervals based on IWGSC reference sequence map, which will be critical QTLs for functional marker development and for improvement of FHB resistance in breeding. Genomic selection (GS) together with marker-assisted selection (MAS) coupling with phenotypic selection will facilitate accumulation of multiple QTLs from different sources to create highly resistant cultivars.

Host Plant Resistance Genes for Fusarium Head Blight: Mapping and Manipulation with Molecular Markers

Crop Science ◽  
2001 ◽  
Vol 41 (3) ◽  
pp. 611-619 ◽  
Author(s):  
F.L. Kolb ◽  
G‐H. Bai ◽  
G.J. Muehlbauer ◽  
J.A. Anderson ◽  
K.P. Smith ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Fusarium Head Blight ◽  
Host Plant ◽  
Resistance Genes ◽  
Plant Resistance ◽  
Host Plant Resistance ◽  
Head Blight

Screening of an EMS mutagenized population of a wheat cultivar susceptible to Fusarium head blight identifies resistant variants

Plant Disease ◽  
2021 ◽  
Author(s):  
Bhavit Chhabra ◽  
Lovepreet Singh ◽  
Sydney Wallace ◽  
Adam Schoen ◽  
Yanhong Dong ◽  
...  
Keyword(s):  
Fusarium Head Blight ◽  
Wheat Cultivar ◽  
Genetic Resistance ◽  
Field Testing ◽  
Head Blight ◽  
Point Of Sale ◽  
Alternative Approaches ◽  
Small Grains ◽  
Fhb Resistance ◽  
Mutant Lines

Fusarium head blight (FHB) primarily caused by Fusarium graminearum is a key disease of small grains. Diseased spikes show symptoms of premature bleaching shortly after infection and have aborted or shriveled seeds, resulting in reduced yields. The fungus also deteriorates quality and safety of the grain due to production of mycotoxins, especially deoxynivalenol (DON), which can result in grain being docked or rejected at the point of sale. Genetic host resistance to FHB is quantitative and no complete genetic resistance against this devastating disease is available. Alternative approaches to develop new sources of FHB resistance are needed. In this study, we performed extensive forward genetic screening of the M4 generation of an EMS induced mutagenized population of cultivar Jagger to isolate variants with FHB resistance. In field testing, 74 mutant lines were found to have resistance against FHB spread and 30 lines out of these also had low DON content. Subsequent testing over two years in controlled greenhouse conditions revealed ten M6 lines showing significantly lower FHB spread. Seven and six lines out of those 10 lines also had reduced DON content and lower FDKs, respectively. Future endeavors will include identification of the mutations that led to resistance in these variants.

scholarly journals Alien chromatin but not Fhb7 confers Fusarium head blight resistance in wheat breeding

2021 ◽  
Author(s):  
Xianrui Guo ◽  
Qinghua Shi ◽  
Jing Yuan ◽  
Mian Wang ◽  
Jing Wang ◽  
...  
Keyword(s):  
Fusarium Head Blight ◽  
Fusarium Head Blight Resistance ◽  
Resistance Breeding ◽  
Wheat Breeding ◽  
Glutathione S Transferase ◽  
Head Blight ◽  
Fhb Resistance ◽  
Translocation Lines ◽  
High Level ◽  
Thinopyrum Elongatum

AbstractFusarium head blight (FHB), caused by Fusarium species, seriously threaten global wheat production. Three wheat-Th.elongatum FHB resistant translocation lines have been developed and used for breeding. Transcriptomic analysis identified a derivative glutathione S-transferase transcript T26102, which was homologous to Fhb7 and induced dramatically by Fusarium graminearum. Homologs of Fhb7 were detected in several genera in Triticeae, including Thinopyrum, Elymus, Leymus, Pseudoroegeria and Roegeria. Several wheat-Thinopyrum translocation lines carrying Fhb7 remain susceptible to FHB, and transgenic plants overexpressing the T26102 on different backgrounds did not improve the FHB resistance. Taken as a whole, we show the application of the chromatin derived from diploid Thinopyrum elongatum successfully conferring wheat with high level FHB resistance independent of the Fhb7.One Sentence SummaryThinopyrum elongatum chromatin from 7EL was successfully applied to wheat FHB resistance breeding, but the resistant gene other than the reported Fhb7 remained unknown.

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