Relationships among durum wheat accessions. II. A comparison of molecular and pedigree information

Genome ◽  
2007 ◽  
Vol 50 (4) ◽  
pp. 385-399 ◽  
Author(s):  
M. Maccaferri ◽  
M.C. Sanguineti ◽  
C. Xie ◽  
J.S.C. Smith ◽  
R. Tuberosa
Keyword(s):  
Durum Wheat ◽  
Simple Sequence Repeat ◽  
Genetic Relatedness ◽  
Fragment Length Polymorphism ◽  
Pedigree Information ◽  
Gene Pools ◽  
Haplotype Sharing ◽  
Coefficient Of Parentage ◽  
Wheat Gene ◽  
Simple Sequence

The study of direct ancestry relationships provides information with which to determine essential derivation. SSR profiles were used to determine the pattern of relatedness among 134 durum wheat accessions, representing the most important modern durum wheat gene pools. Simple sequence repeat (SSR)- and amplified fragment length polymorphism (AFLP)-based genetic similarities among cultivars with accurate pedigrees were compared with pedigree-based coefficients of parentage. Sizeable departures of molecular similarities from the expected ones were observed, indicating the unreliability of inferring the pattern of genetic relatedness from the coefficient of parentage. Case studies consisting of parent–progeny cultivar trios and pairs, identified on the basis of their registered pedigree, were studied to evaluate the probability of ancestry of each progeny cultivar, compared with all the remaining accessions. Rare alleles and haplotype sharing were also explored. When the results did not agree with the registered parentages, SSR markers provided information with which to identify the most probable parents (or the corresponding “breeding lineages”) in the collection.

Characterization of the Russian beef cattle breed gene pools using inter simple sequence repeat DNA analysis (ISSR analysis)

2016 ◽  
Vol 52 (9) ◽  
pp. 963-968 ◽  
Author(s):  
G. E. Sulimova ◽  
V. N. Voronkova ◽  
A. V. Perchun ◽  
I. F. Gorlov ◽  
A. V. Randelin ◽  
...  
Keyword(s):  
Simple Sequence Repeat ◽  
Dna Analysis ◽  
Cattle Breed ◽  
Inter Simple Sequence Repeat ◽  
Gene Pools ◽  
Issr Analysis ◽  
Repeat Dna ◽  
Simple Sequence ◽  
Simple Sequence Repeat Dna

scholarly journals Comparative Analysis of Genetic Similarity between Perennial Ryegrass Genotypes Investigated With AFLPs, ISSRs, RAPDs and SSRs

2011 ◽  
Vol 42 (No. 3) ◽  
pp. 87-94 ◽  
Author(s):  
U.K. Posselt ◽  
P. Barre ◽  
G. Brazauskas ◽  
L.B. Turner
Keyword(s):  
Perennial Ryegrass ◽  
Simple Sequence Repeat ◽  
Fragment Length Polymorphism ◽  
Genetic Relationships ◽  
Random Amplified Polymorphic Dna ◽  
Correlation Coefficients ◽  
Inter Simple Sequence Repeat ◽  
Grass Species ◽  
Sequence Repeat ◽  
Simple Sequence

Perennial ryegrass (Lolium perenne L.) is the most important grass species used in temperate grassland agriculture. Our objective was to obtain an overview of the genetic relationships between 20 individual genotypes of perennial ryegrass of diverse origins, using amplified fragment length polymorphism (AFLP), inter-simple sequence repeat (ISSR), random amplified polymorphic DNA (RAPD) and two sets of simple sequence repeat (SSR) markers. All 20 individuals were uniquely fingerprinted by all four marker systems and comparisons were made on the basis of 85 markers each. Mean genetic similarities were estimated at 0.31, 0.43, 0.23 and 0.15 for AFLPs, ISSRs, RAPDs and SSRs, respectively. Cophenetic values resulted in good (AFLP and SSR-B = 0.88) to moderately good fits (ISSR = 0.76, RAPD = 0.70, and SSR-A = 0.79). Comparing the four marker systems to each other, AFLP and SSR-A were correlated best (r = 0.57). All other comparisons revealed rather low correlation coefficients in the Mantel Z test. With twice as many markers cophenetic values increased to a very good fit for AFLPs (0.90) and SSRs (0.92).      

scholarly journals A Genetic Linkage Map of Olive Based on Amplified Fragment Length Polymorphism, Intersimple Sequence Repeat and Simple Sequence Repeat Markers

2010 ◽  
Vol 135 (6) ◽  
pp. 548-555 ◽  
Author(s):  
Bouchaib Khadari ◽  
Amal Zine El Aabidine ◽  
Cinderella Grout ◽  
Inès Ben Sadok ◽  
Agnès Doligez ◽  
...  
Keyword(s):  
Linkage Map ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Simple Sequence Repeat ◽  
Fragment Length Polymorphism ◽  
Amplified Fragment Length Polymorphism ◽  
Issr Markers ◽  
Phenotypic Characterization ◽  
Sequence Repeat ◽  
Simple Sequence

A detailed genomic linkage map of the olive [Olea europaea L. ssp. europaea (2x = 2n = 46)] was constructed with a 147 F1 full-sib ‘Olivière’ × ‘Arbequina’ progeny in a two-way pseudo-test cross-mapping configuration. Based on a logarithm of odds threshold of 6 and a maximum recombination fraction of 0.4, maternal and paternal maps were constructed using 222 makers [178 amplified fragment length polymorphism (AFLP), 37 simple sequence repeat (SSR), seven intersimple sequence repeat (ISSR)] and 219 markers (174 AFLP, 39 SSR, 6 ISSR) markers, respectively. The female map regrouped 36 linkage groups (LGs) defining 2210.2 cM of total map length with an average marker spacing 11.2 cM and a maximum gap of 48.5 cM between adjacent markers. The male map contained 31 LGs and covered a distance of 1966.2 cM with an average and a maximum distance between two adjacent markers of 10.3 and 40.4 cM, respectively. Mean LG size was 61.3 and 63.4 cM in the maternal and paternal maps, respectively. The LGs consisted of two to 17 loci (up to 21 loci in the paternal map) and ranged in length from 2.7 to 182 cM (female map) or from 4.1 to 218.1 cM (paternal map). Markers were distributed throughout the maps without any clustering. The total length of the consensus map was 3823.2 cM containing 436 markers distributed into 42 LGs with a mean distance between two adjacent loci of 8.7 cM. Both parental maps and the consensus maps were compared with previously published olive maps. Although not saturated yet, the present maps offer a promising tool for quantitative trait loci mapping because phenotypic characterization of the cross is currently carried out.

Microsatellite markers for common lilac (Syringa vulgaris L.)

2013 ◽  
Vol 11 (3) ◽  
pp. 279-282 ◽  
Author(s):  
Inka Juntheikki-Palovaara ◽  
Kristiina Antonius ◽  
Leena Lindén ◽  
Helena Korpelainen
Keyword(s):  
Microsatellite Markers ◽  
Simple Sequence Repeat ◽  
Inter Simple Sequence Repeat ◽  
Pedigree Information ◽  
The Novel ◽  
Landscape Plant ◽  
Common Lilac ◽  
Genome Screening ◽  
Polymorphic Microsatellite ◽  
Simple Sequence

Common lilac is a popular landscape plant with nearly 2000 cultivars. In the present study, nine novel polymorphic microsatellite markers were developed for common lilac using genome screening with inter-simple sequence repeat primers. Each of the nine markers revealed two to five alleles. The markers were used for genotyping of 75 common lilac shrubs, including 17 accessions that represented named cultivars. A dendrogram, based on the allele profiles of the shrubs, was generally in agreement with the grouping made on the basis of morphological traits, as well as with the pedigree information available. The novel markers appear valuable for differentiation between common lilac cultivars.

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