A genetic linkage map of Pleurotus pulmonarius based on AFLP markers, and localization of the gene region for the sporeless mutation

Genome ◽  
2009 ◽  
Vol 52 (5) ◽  
pp. 438-446 ◽  
Author(s):  
Yasuhito Okuda ◽  
Shigeyuki Murakami ◽  
Teruyuki Matsumoto
Keyword(s):  
Linkage Map ◽  
Mating Type ◽  
Genetic Linkage ◽  
Genetic Linkage Map ◽  
Natural Populations ◽  
Aflp Markers ◽  
Gene Region ◽  
Pleurotus Pulmonarius ◽  
Type Factor ◽  
Enormous Number

In the cultivation of edible mushrooms, including Pleurotus pulmonarius (Fr.) Quel., the enormous number of spores produced by fruiting bodies can adversely affect mushroom growers’ health, mushroom cultivation facilities, and the genetic diversity of natural populations. In this study, we constructed a primary genetic linkage map and identified the locus associated with the sporulation-deficient (sporeless) mutation of P. pulmonarius using 150 progeny isolates derived from a cross between sporeless and wild-type isolates. Based on the segregation of 300 AFLP markers, two mating-type factors, and the sporeless trait, a linkage map was generated consisting of 12 linkage groups. The map covered a total genetic distance of 971 cM, with an average marker interval of 5.2 cM. The gene region responsible for the sporeless mutation was located in linkage group II including 40 AFLP markers and the A mating-type factor locus. Of these markers, the nearest marker to the sporeless locus was located 1.4 cM away. Construction of this P. pulmonarius genetic linkage map and identification of markers that are closely linked to the sporeless locus will facilitate marker-assisted selective breeding of a sporeless strain with economically important traits.

scholarly journals A genetic linkage map of Pleurotus tuoliensis integrated with physical mapping of the de novo sequenced genome and the mating type loci

BMC Genomics ◽  
2018 ◽  
Vol 19 (1) ◽  
Author(s):  
Wei Gao ◽  
Jibin Qu ◽  
Jinxia Zhang ◽  
Anton Sonnenberg ◽  
Qiang Chen ◽  
...  
Keyword(s):  
Linkage Map ◽  
Mating Type ◽  
Physical Mapping ◽  
Genetic Linkage ◽  
Genetic Linkage Map ◽  
De Novo

A genetic linkage map of Lens sp. based on microsatellite and AFLP markers and the localization of fusarium vascular wilt resistance

2005 ◽  
Vol 110 (4) ◽  
pp. 669-677 ◽  
Author(s):  
A. Hamwieh ◽  
S. M. Udupa ◽  
W. Choumane ◽  
A. Sarker ◽  
F. Dreyer ◽  
...  
Keyword(s):  
Linkage Map ◽  
Genetic Linkage ◽  
Genetic Linkage Map ◽  
Aflp Markers ◽  
Vascular Wilt ◽  
Wilt Resistance

A first genetic linkage map of bluegill sunfish (Lepomis macrochirus) using AFLP markers

2009 ◽  
Vol 18 (5) ◽  
pp. 825-835 ◽  
Author(s):  
Wei-Ji Wang ◽  
Han-Ping Wang ◽  
Hong Yao ◽  
Geoff K. Wallat ◽  
Laura G. Tiu ◽  
...  
Keyword(s):  
Linkage Map ◽  
Genetic Linkage ◽  
Genetic Linkage Map ◽  
Lepomis Macrochirus ◽  
Aflp Markers ◽  
Bluegill Sunfish

CONSTRUCTION OF A MOLECULAR GENETIC LINKAGE MAP FOR LONGAN BASED ON RAPD, ISSR, SRAP AND AFLP MARKERS

2010 ◽  
pp. 141-148 ◽  
Author(s):  
Y.S. Guo ◽  
C.J. Liu ◽  
Y.H. Zhao ◽  
J.H. Xie ◽  
P.R. Ren ◽  
...  
Keyword(s):  
Linkage Map ◽  
Genetic Linkage ◽  
Genetic Linkage Map ◽  
Molecular Genetic ◽  
Aflp Markers

Construction of a genetic linkage map and identification of AFLP markers for resistance to root-knot nematodes in peach rootstocks

Genome ◽  
1998 ◽  
Vol 41 (2) ◽  
pp. 199-207 ◽  
Author(s):  
Zhen-Xiang Lu ◽  
B Sosinski ◽  
G L Reighard ◽  
W V Baird ◽  
A G Abbott
Keyword(s):  
Linkage Map ◽  
Genetic Linkage ◽  
Genetic Linkage Map ◽  
Prunus Persica ◽  
Aflp Marker ◽  
Aflp Markers ◽  
Single Linkage ◽  
Root Knot Nematodes ◽  
Group I ◽  
Peach Rootstocks

A genetic linkage map for peach (Prunus persica (L.) Batsch) rootstocks has been constructed using amplified fragment length polymorphism (AFLP) markers in 55 F2 individuals derived from the cross Lovell x Nemared. From 21 different primer combinations, a total of 169 AFLP markers were scored, of which, 153 were assigned to 15 linkage groups covering 1297 centimorgans (cM) of the peach nuclear genome. The average interval between these markers was 9.1 cM. Two genes (Mi and Mij) involved in resistance to root-knot nematodes (Meloidogyne incognita (Kofoid and White) Chitwood and Meloidogyne javanica (Treub) Chitwood) were mapped to a single linkage group (Group I). These two loci were separated by a 16.5-cM interval. One codominant AFLP marker (EAA/MCAT10) was tightly linked to the Mij locus (3.4 cM), and a dominant AFLP marker (EAT/MCAT2) was found to be closely associated with the Mi locus (6.0 cM). These markers are being studied for utilization in peach rootstock breeding with marker-assisted selection.Key words: peach rootstocks, root-knot nematodes, resistance, AFLP, mapping.

A genetic linkage map of swimming crab (Portunus trituberculatus) based on SSR and AFLP markers

Aquaculture ◽  
2012 ◽  
Vol 344-349 ◽  
pp. 66-81 ◽  
Author(s):  
Lei Liu ◽  
Jian Li ◽  
Ping Liu ◽  
Fazhen Zhao ◽  
Baoquan Gao ◽  
...  
Keyword(s):  
Linkage Map ◽  
Genetic Linkage ◽  
Genetic Linkage Map ◽  
Aflp Markers ◽  
Portunus Trituberculatus ◽  
Swimming Crab

scholarly journals GENETIC LINKAGE MAP FOR WATERMELON: SEGREGATION AND DISTRIBUTION OF DNA MARKERS

HortScience ◽  
2005 ◽  
Vol 40 (3) ◽  
pp. 872a-872
Author(s):  
A. Levi ◽  
C. E. Thomas ◽  
J. Thies ◽  
A. Simmons ◽  
Y. Xu ◽  
...  
Keyword(s):  
Linkage Map ◽  
Genetic Linkage ◽  
Rapd Markers ◽  
Genetic Linkage Map ◽  
Genetic Distances ◽  
Aflp Markers ◽  
F2 Population ◽  
Skewed Segregation ◽  
Linkage Distance ◽  
Map Distance

Genetic linkage map is being constructed for watermelon based on a testcross population and an F2 population. About 51.0% and 31.8% of the markers in the testcross and F2 populations are skewed form the expected segregation ratios. AFLP markers appeared to be clustered on linkage regions, while ISSR and RAPD markers are randomly dispersed on the genome. AFLP markers also have greater genetic distances as compared with ISSR and RAPD markers, resulting in significant increase of map distance. An initial genetic map (based on the testcross population) that contains 27 ISSR and 141 RAPD markers has a total linkage distance of 1,166.2 cM. The addition of 2 ISSR, 8 RAPD and 77 AFLP markers increased the genetic distance of the map to 2,509.9 cM. Similar results with AFLP markers were also shown in mapping experiments with an F2S7 recombinant inbred line (RIL) population that was recently constructed for watermelon. Although the skewed segregation, marker order appeared to be consistent in linkage groups of the testcross and the F2 population. Experiments with SSR, and EST markers are being conducted to saturate the linkage map of watermelon genome.

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