Characterization of telomeres in Hordeum vulgare chromosomes by in situ hybridization. II. Healed broken chromosomes in telotrisomic 4L and acrotrisomic 4L4S lines

Genome ◽  
1992 ◽  
Vol 35 (6) ◽  
pp. 975-980 ◽  
Author(s):  
Shaoke Wang ◽  
Nora L. V. Lapitan ◽  
Marion Roder ◽  
Takumi Tsuchiya
Keyword(s):  
Arabidopsis Thaliana ◽  
In Situ Hybridization ◽  
Hordeum Vulgare ◽  
Telomeric Sequences ◽  
The Absolute ◽  
The Cross ◽  
The Stability ◽  
Different Parts

The ends of barley chromosomes hybridize in situ to the telomeric sequences of Arabidopsis thaliana. It was confirmed that the cross-hybridizing sequences in barley are found at the absolute ends of the chromosomes by exonuclease Bal31 digestion. The Bal31 experiments also indicated that telomere-like sequences do not occur in high copies at interstitial sites in barley. To determine whether healing of broken chromosomes occurred in aneuploid lines of barley containing extra chromosomes with breakages in different parts, in situ hybridization with the A. thaliana telomere on telotrisomic 4L and acrotrisomic 4L4S lines was conducted. Telosome 4L possesses breaks in the centromere and in an interstitial location in the long arm, while acrosome 4L4S possesses interstitial breaks in both long and short arms. In situ hybridization revealed the presence of telomere sequences on both broken ends of telosome 4L and acrosome 4L4S. In telosome 4L, telomere sequences were present even at the broken site of the centromere. These results show that broken ends of barley chromosomes were healed. Such healing may explain the stability of these chromosomes through many generations.Key words: telomere, centromere, telosome, acrosome, acrotrisomic, telotrisomic.

Identification of new winter wheat – winter barley addition lines (6HS and 7H) using fluorescence in situ hybridization and the stability of the whole ‘Martonvásári 9 kr1’ – ‘Igri’ addition set

Genome ◽  
2010 ◽  
Vol 53 (1) ◽  
pp. 35-44 ◽  
Author(s):  
É. Szakács ◽  
M. Molnár-Láng
Keyword(s):  
In Situ Hybridization ◽  
Winter Wheat ◽  
Fluorescence In Situ Hybridization ◽  
Morphological Characterization ◽  
Winter Barley ◽  
Addition Lines ◽  
Disomic Addition Lines ◽  
The Stability

A previous paper reported the development of disomic addition lines (2H, 3H, 4H, and 1HS isochromosomic) from hybrids between the winter wheat ‘Martonvásári 9 kr1’ and the two-rowed winter barley cultivar ‘Igri’. The present paper describes the isolation of two new additions, the 7H disomic and 6HS ditelosomic additions, using fluorescence in situ hybridization with the repetitive DNA probes Afa-family and HvT01. The identification of the barley chromosomes in the wheat genome was confirmed with simple sequence repeat markers. The morphological characterization of the new addition lines is also discussed. Studies of the genetic stability of the whole set (2H, 3H, 4H, 7H, 1HS iso, 6HS) of ‘Martonvásári 9 kr1’ – ‘Igri’ additions revealed that the most stable disomic additions are 2H and 3H and the most unstable line is the 1HS isochromosomic addition.

scholarly journals Brief communication. In situ hybridization mapping of genes in Hordeum vulgare L.

1998 ◽  
Vol 89 (4) ◽  
pp. 366-370 ◽  
Author(s):  
G Butnaru ◽  
J Chen ◽  
P Goicoechea ◽  
JP Gustafson
Keyword(s):  
In Situ Hybridization ◽  
Hordeum Vulgare ◽  
Hordeum Vulgare L ◽  
Hybridization Mapping

Identification and characterization of normal length nonfluorescent Y chromosomes: cytogenetic analysis, Southern hybridization and non-isotopic in situ hybridization

1990 ◽  
Vol 85 (6) ◽  
pp. 569-575 ◽  
Author(s):  
Frank Speleman ◽  
Bart Van der Auwera ◽  
Kathelijne Mangelschots ◽  
Miet Vercruyssen ◽  
Ton Raap ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Cytogenetic Analysis ◽  
Southern Hybridization ◽  
Normal Length ◽  
Y Chromosomes ◽  
Identification And Characterization

Molecular Cytogenetic Characterization of the DiGeorge Syndrome Region Using Fluorescence in Situ Hybridization

Genomics ◽  
1993 ◽  
Vol 17 (2) ◽  
pp. 403-407 ◽  
Author(s):  
Elizabeth A. Lindsay ◽  
Stephanie Halford ◽  
Roy Wadey ◽  
Peter J. Scambler ◽  
Antonio Baldini
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Digeorge Syndrome ◽  
Molecular Cytogenetic ◽  
Cytogenetic Characterization

Physical mapping of barley genes using an ultrasensitive fluorescence in situ hybridization technique

Genome ◽  
2004 ◽  
Vol 47 (1) ◽  
pp. 179-189 ◽  
Author(s):  
J L Stephens ◽  
S E Brown ◽  
N L.V Lapitan ◽  
D L Knudson
Keyword(s):  
In Situ Hybridization ◽  
Hordeum Vulgare ◽  
Fluorescence In Situ Hybridization ◽  
Linkage Map ◽  
Physical Mapping ◽  
Physical Map ◽  
Primary Objective ◽  
Hybridization Technique ◽  
Hordeum Vulgare L

The primary objective of this study was to elucidate gene organization and to integrate the genetic linkage map for barley (Hordeum vulgare L.) with a physical map using ultrasensitive fluorescence in situ hybridization (FISH) techniques for detecting signals from restriction fragment length polymorphism (RFLP) clones. In the process, a single landmark plasmid, p18S5Shor, was constructed that identified and oriented all seven of the chromosome pairs. Plasmid p18S5Shor was used in all hybridizations. Fourteen cDNA probes selected from the linkage map for barley H. vulgare 'Steptoe' × H. vulgare 'Morex' (Kleinhofs et al. 1993) were mapped using an indirect tyramide signal amplification technique and assigned to a physical location on one or more chromosomes. The haploid barley genome is large and a complete physical map of the genome is not yet available; however, it was possible to integrate the linkage map and the physical locations of these cDNAs. An estimate of the ratio of base pairs to centimorgans was an average of 1.5 Mb/cM in the distal portions of the chromosome arms and 89 Mb/cM near the centromere. Furthermore, while it appears that the current linkage maps are well covered with markers along the length of each arm, the physical map showed that there are large areas of the genome that have yet to be mapped.Key words: Hordeum vulgare, barley, physical mapping, FISH, cDNA, genetics, linkage, chromosome, BACs.

scholarly journals Chromosome Mapping of Repetitive Sequences inRachycentron canadum(Perciformes: Rachycentridae): Implications for Karyotypic Evolution and Perspectives for Biotechnological Uses

2011 ◽  
Vol 2011 ◽  
pp. 1-8 ◽  
Author(s):  
Uedson Pereira Jacobina ◽  
Marcelo de Bello Cioffi ◽  
Luiz Gustavo Rodrigues Souza ◽  
Leonardo Luiz Calado ◽  
Manoel Tavares ◽  
...  
Keyword(s):  
Repetitive Sequences ◽  
Chromosome Mapping ◽  
Karyotypic Evolution ◽  
Dapi Staining ◽  
Telomeric Sequences ◽  
Replication Banding ◽  
The Family ◽  
Early Replication

The cobia,Rachycentron canadum, a species of marine fish, has been increasingly used in aquaculture worldwide. It is the only member of the family Rachycentridae (Perciformes) showing wide geographic distribution and phylogenetic patterns still not fully understood. In this study, the species was cytogenetically analyzed by different methodologies, including Ag-NOR and chromomycin A3(CMA3)/DAPI staining, C-banding, early replication banding (RGB), andin situfluorescent hybridization with probes for 18S and 5S ribosomal genes and for telomeric sequences (TTAGGG)n. The results obtained allow a detailed chromosomal characterization of the Atlantic population. The chromosome diversification found in the karyotype of the cobia is apparently related to pericentric inversions, the main mechanism associated to the karyotypic evolution of Perciformes. The differential heterochromatin replication patterns found were in part associated to functional genes. Despite maintaining conservative chromosomal characteristics in relation to the basal pattern established for Perciformes, some chromosome pairs in the analyzed population exhibit markers that may be important for cytotaxonomic, population, and biodiversity studies as well as for monitoring the species in question.

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