Inheritance of random amplified polymorphic DNA markers in an interspecific cross in the genus Stylosanthes

Genome ◽  
1993 ◽  
Vol 36 (1) ◽  
pp. 50-56 ◽  
Author(s):  
Kemal Kazan ◽  
John M. Manners ◽  
Don F. Cameron
Keyword(s):  
Polymerase Chain Reaction ◽  
Dna Sequences ◽  
Rapd Markers ◽  
Dna Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Interspecific Cross ◽  
Parental Origin ◽  
Chain Reaction ◽  
Stylosanthes Hamata ◽  
Polymerase Chain

The inheritance of random amplified polymorphic DNA (RAPD) markers generated via the polymerase chain reaction amplification of genomic DNA sequences in an F2 family of an interspecific cross between Stylosanthes hamata and S. scabra was investigated. An initial comparison between the parental species, S. hamata cv. Verano and S. scabra cv. Fitzroy, demonstrated that 34% of detected RAPD bands were polymorphic. Of 90 primers tested, 35 showed relatively simple and reliably scorable polymorphisms and were used for segregation analysis. Sixty F2 individuals were scored for the segregation of 73 RAPD markers and 55 of these markers fit a 3:1 ratio. Segregation of eight other RAPD markers deviated significantly from a 3:1 ratio. There was no bias in the inheritance of RAPD markers regarding parental origin of the segregating RAPD markers. Linkage analysis revealed 10 linkage groups containing a total of 44 RAPD loci. Another 10 RAPD markers (7 of maternal origin) that were polymorphic between the parents did not segregate in the F2 population. One of the maternally inherited RAPD bands hybridized to chloroplast DNA. Analysis of RAPD loci by DNA hybridization indicated that mainly repeated sequences were amplified. These data indicate that RAPDs are useful genetic markers in Stylosanthes spp. and they may be suitable for genetic mapping.Key words: genetic mapping, molecular markers, polymerase chain reaction, Stylosanthes hamata, Stylosanthes scabra.

Random amplified polymorphic DNA analysis in Hordeum species

Genome ◽  
1993 ◽  
Vol 36 (6) ◽  
pp. 1029-1031 ◽  
Author(s):  
Juan Manuel González ◽  
Esther Ferrer
Keyword(s):  
Polymerase Chain Reaction ◽  
Dna Polymerase ◽  
Dna Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Chain Reaction ◽  
Fragment Pattern ◽  
Polymerase Chain ◽  
Hordeum Species

Random amplified polymorphic DNA analysis was performed by applying a set of 13 arbitrary 10-mer primers to 19 Hordeum species and subspecies. High levels of variation in fragment pattern were observed both within and among species with most of the primers used. Genetic similarities between accessions and species were calculated from the fragment patterns. The resulting phenograms confirmed previous relationships among the Hordeum species.Key words: random amplified polymorphic DNA, polymerase chain reaction, polymorphism, Hordeum.

scholarly journals Detection and Differentiation of Phaeoisariopsis griseola Isolates with the Polymerase Chain Reaction and Group-Specific Primers

Plant Disease ◽  
1999 ◽  
Vol 83 (1) ◽  
pp. 37-42 ◽  
Author(s):  
P. Guzmán ◽  
P. Gepts ◽  
S. Temple ◽  
A. B. C. Mkandawire ◽  
R. L. Gilbertson
Keyword(s):  
Polymerase Chain Reaction ◽  
Dna Sequences ◽  
Random Amplified Polymorphic Dna ◽  
Extraction Methods ◽  
Chain Reaction ◽  
Phaeoisariopsis Griseola ◽  
Geographical Regions ◽  
Polymerase Chain ◽  
Dna Extraction Methods ◽  
Group Specific Primer

Specific detection of the two major groups of Phaeoisariopsis griseola(Andean and Mesoamerican) from infected common bean (Phaseolus vulgaris) leaves was achieved by amplification of different-sized DNA fragments with polymerase chain reaction (PCR) using group-specific primer pairs. These primer pairs were designed based on DNA sequences of cloned random amplified polymorphic DNA (RAPD) fragments. Using this method, P. griseola isolates from diverse geographical regions (five countries) were differentiated into the two previously established groups. Various sources of fungal tissue and DNA extraction methods were tested in order to develop a rapid PCR-based method to detect and differentiate P. griseola isolates. A simple and rapid sonication method was developed that allowed for PCR detection of P. griseola from mycelia or synnemata and conidia collected from angular leaf spot lesions on bean leaves.

Genetic segregation of random amplified polymorphic DNA in diploid cultivated alfalfa

Genome ◽  
1992 ◽  
Vol 35 (1) ◽  
pp. 84-87 ◽  
Author(s):  
C. S. Echt ◽  
L. A. Erdahl ◽  
T. J. McCoy
Keyword(s):  
Polymerase Chain Reaction ◽  
Rapd Markers ◽  
Genome Mapping ◽  
Polymerase Chain Reaction Amplification ◽  
Random Amplified Polymorphic Dna ◽  
Rapid Development ◽  
Arbitrary Sequence ◽  
Chain Reaction ◽  
Backcross Population ◽  
Polymerase Chain

Polymerase chain reaction was used, with single 10-mer primers of arbitrary sequence, to amplify random regions of genomic DNA from a diploid cultivated alfalfa backcross population. Segregation of the random amplified polymorphic DNA (RAPD) fragments was analysed to determine if RAPD markers are suitable for use as genetic markers. Of the 19 primers tested, 13 amplified a total of 37 polymorphic fragments, of which 28 (76%) segregated as dominant Mendelian traits. RAPD markers appear useful for the rapid development of genetic information in species like alfalfa where little information currently exists or is difficult to obtain.Key words: random amplified polymorphic DNA, polymerase chain reaction amplification, genome mapping, restriction fragment length polymorphism, Medicago sativa.

scholarly journals Identification of Citrus Chimeras by RAPD Markers

HortScience ◽  
1995 ◽  
Vol 30 (6) ◽  
pp. 1276-1278 ◽  
Author(s):  
Kuniaki Sugawara ◽  
Atsushi Oowada ◽  
Takaya Moriguchi ◽  
Mitsuo Omura
Keyword(s):  
Polymerase Chain Reaction ◽  
Rapd Markers ◽  
Random Amplified Polymorphic Dna ◽  
Chain Reaction ◽  
Reaction Conditions ◽  
Polymerase Chain

Random amplified polymorphic DNA (RAPD) markers were used to detect chimerism of citrus cultivars. Polymerase chain reaction conditions suitable for discriminating citrus chimeras were determined. Primers that produced consistent and repeatable bands that differed between the parental cultivars were chosen to create discriminating band patterns. Our results show that selected 12-mer primers can be useful for identifying the four citrus chimeras tested using RAPD technology.

A comparison of Echinostoma trivolvis and E. caproni using random amplified polymorphic DNA analysis

1995 ◽  
Vol 69 (3) ◽  
pp. 263-264 ◽  
Author(s):  
T. Fujino ◽  
Y. Takahashi ◽  
B. Fried
Keyword(s):  
Polymerase Chain Reaction ◽  
Dna Polymerase ◽  
Genomic Dna ◽  
Dna Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Chain Reaction ◽  
Rapd Pcr ◽  
Polymerase Chain ◽  
Echinostoma Trivolvis

AbstractThe random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) technique was applied to two closely-related echinostome species, Echinostoma trivolvis and E. caproni, to demonstrate interspecffic polymorphisms of genomic DNA. Band patterns generated using five individual primers showed that these two echinostomes were genetically distinct, although they share genomic DNA to some extent.

Using a Polymerase Chain Reaction as a Complementary Test to Improve the Detection of Dermatophyte Fungus in Nails

2014 ◽  
Vol 104 (3) ◽  
pp. 233-237 ◽  
Author(s):  
María José Iglesias Sánchez ◽  
Ana María Pérez Pico ◽  
Félix Marcos Tejedor ◽  
María Jesús Iglesias Sánchez ◽  
Raquel Mayordomo Acevedo
Keyword(s):  
Polymerase Chain Reaction ◽  
Dna Sequences ◽  
Classical Method ◽  
Clinical Manifestations ◽  
Culture Method ◽  
Clinical Samples ◽  
Chain Reaction ◽  
Nested Polymerase Chain Reaction ◽  
Traditional Procedure ◽  
Polymerase Chain

Background Dermatomycoses are a group of pathologic abnormalities frequently seen in clinical practice, and their prevalence has increased in recent decades. Diagnostic confirmation of mycotic infection in nails is essential because there are several pathologic conditions with similar clinical manifestations. The classical method for confirming the presence of fungus in nail is microbiological culture and the identification of morphological structures by microscopy. Methods We devised a nested polymerase chain reaction (PCR) that amplifies specific DNA sequences of dermatophyte fungus that is notably faster than the 3 to 4 weeks that the traditional procedure takes. We compared this new technique and the conventional plate culture method in 225 nail samples. The results were subjected to statistical analysis. Results We found concordance in 78.2% of the samples analyzed by the two methods and increased sensitivity when simultaneously using the two methods to analyze clinical samples. Now we can confirm the presence of dermatophyte fungus in most of the positive samples in just 24 hours, and we have to wait for the result of culture only in negative PCR cases. Conclusions Although this PCR cannot, at present, substitute for the traditional culture method in the detection of dermatophyte infection of the nails, it can be used as a complementary technique because its main advantage lies in the significant reduction of time used for diagnosis, in addition to higher sensitivity.

Polymerase chain reaction based mapping of rye involving repeated DNA sequences

Genome ◽  
1992 ◽  
Vol 35 (4) ◽  
pp. 621-626 ◽  
Author(s):  
Peter M. Rogowsky ◽  
Ken W. Shepherd ◽  
Peter Langridge
Keyword(s):  
Polymerase Chain Reaction ◽  
Dna Sequences ◽  
Chromosome 1 ◽  
Repeated Dna ◽  
Chain Reaction ◽  
Pcr Marker ◽  
Banding Patterns ◽  
Repeated Dna Sequences ◽  
Cereal Rye ◽  
Polymerase Chain

A novel type of polymerase chain reaction (PCR) marker was developed for the mapping of cereal rye (Secale cereale). Primer pairs were synthesized targeting the insertion sites of three individual copies of the R173 family of rye specific repeated DNA sequences. While one primer was derived from a sequence within the respective R173 element, the second primer corresponded to a flanking region. The complex banding patterns obtained in rye allowed not only the mapping of the three R173 elements to certain chromosome regions of 1RS (the short arm of rye chromosome 1) but also the mapping of an additional 3–10 easily identifiable bands per primer pair to other rye chromosomes. Linkage mapping of a polymorphic 1R band derived from three rye cultivars demonstrated the presence of nonallelic, dominant markers in two independent crosses. Because of the high copy number of the R173 family (15 000 copies per diploid rye genome), its dispersion over the entire length of all chromosomes and the high number of markers obtained per primer pair, PCR markers based on the R173 family provide an almost unlimited source for well-spaced markers in rye mapping.Key words: polymerase chain reaction, mapping, repetitive DNA sequences, wheat, rye.

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