A comparison of Echinostoma trivolvis and E. caproni using random amplified polymorphic DNA analysis

1995 ◽  
Vol 69 (3) ◽  
pp. 263-264 ◽  
Author(s):  
T. Fujino ◽  
Y. Takahashi ◽  
B. Fried
Keyword(s):  
Polymerase Chain Reaction ◽  
Dna Polymerase ◽  
Genomic Dna ◽  
Dna Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Chain Reaction ◽  
Rapd Pcr ◽  
Polymerase Chain ◽  
Echinostoma Trivolvis

AbstractThe random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) technique was applied to two closely-related echinostome species, Echinostoma trivolvis and E. caproni, to demonstrate interspecffic polymorphisms of genomic DNA. Band patterns generated using five individual primers showed that these two echinostomes were genetically distinct, although they share genomic DNA to some extent.

Random amplified polymorphic DNA analysis in Hordeum species

Genome ◽  
1993 ◽  
Vol 36 (6) ◽  
pp. 1029-1031 ◽  
Author(s):  
Juan Manuel González ◽  
Esther Ferrer
Keyword(s):  
Polymerase Chain Reaction ◽  
Dna Polymerase ◽  
Dna Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Chain Reaction ◽  
Fragment Pattern ◽  
Polymerase Chain ◽  
Hordeum Species

Random amplified polymorphic DNA analysis was performed by applying a set of 13 arbitrary 10-mer primers to 19 Hordeum species and subspecies. High levels of variation in fragment pattern were observed both within and among species with most of the primers used. Genetic similarities between accessions and species were calculated from the fragment patterns. The resulting phenograms confirmed previous relationships among the Hordeum species.Key words: random amplified polymorphic DNA, polymerase chain reaction, polymorphism, Hordeum.

DNA FINGERPRINTING OF SINGLE APHID EMBRYOS BY RANDOM AMPLIFIED POLYMORPHIC DNA POLYMERASE CHAIN REACTION (RAPD–PCR)

1999 ◽  
Vol 131 (2) ◽  
pp. 229-230 ◽  
Author(s):  
C.K. Chan ◽  
D.J. Petersen ◽  
T.C. Vrain
Keyword(s):  
Polymerase Chain Reaction ◽  
Dna Polymerase ◽  
Acyrthosiphon Pisum ◽  
Random Amplified Polymorphic Dna ◽  
Aphis Gossypii ◽  
Aphis Fabae ◽  
Chain Reaction ◽  
Rapd Pcr ◽  
Laboratory Colonies ◽  
Polymerase Chain

Extraction of DNA from whole aphids, in combination with random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) (Williams et al. 1990) markers can detect interspecific and intraspecific genetic variation (Black et al. 1992; Cenis et al. 1993). However, these techniques entail destructive sampling of fresh or preserved specimens. To allow experimental replication from a single sample while preserving the same aphid for morphometrical or karyotyping analyses, we describe a technique for RAPD-PCR using DNA from single aphid embryos. We evaluated the usefulness and reliability of single-embryo analysis, using four species of our laboratory colonies, namely Acyrthosiphon pisum (Harris), Aphis fabae Scopoli, Aphis frangulae group, and Aphis gossypii Glover.

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