scholarly journals Identification of Citrus Chimeras by RAPD Markers

HortScience ◽  
1995 ◽  
Vol 30 (6) ◽  
pp. 1276-1278 ◽  
Author(s):  
Kuniaki Sugawara ◽  
Atsushi Oowada ◽  
Takaya Moriguchi ◽  
Mitsuo Omura
Keyword(s):  
Polymerase Chain Reaction ◽  
Rapd Markers ◽  
Random Amplified Polymorphic Dna ◽  
Chain Reaction ◽  
Reaction Conditions ◽  
Polymerase Chain

Random amplified polymorphic DNA (RAPD) markers were used to detect chimerism of citrus cultivars. Polymerase chain reaction conditions suitable for discriminating citrus chimeras were determined. Primers that produced consistent and repeatable bands that differed between the parental cultivars were chosen to create discriminating band patterns. Our results show that selected 12-mer primers can be useful for identifying the four citrus chimeras tested using RAPD technology.

Inheritance of random amplified polymorphic DNA markers in an interspecific cross in the genus Stylosanthes

Genome ◽  
1993 ◽  
Vol 36 (1) ◽  
pp. 50-56 ◽  
Author(s):  
Kemal Kazan ◽  
John M. Manners ◽  
Don F. Cameron
Keyword(s):  
Polymerase Chain Reaction ◽  
Dna Sequences ◽  
Rapd Markers ◽  
Dna Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Interspecific Cross ◽  
Parental Origin ◽  
Chain Reaction ◽  
Stylosanthes Hamata ◽  
Polymerase Chain

The inheritance of random amplified polymorphic DNA (RAPD) markers generated via the polymerase chain reaction amplification of genomic DNA sequences in an F2 family of an interspecific cross between Stylosanthes hamata and S. scabra was investigated. An initial comparison between the parental species, S. hamata cv. Verano and S. scabra cv. Fitzroy, demonstrated that 34% of detected RAPD bands were polymorphic. Of 90 primers tested, 35 showed relatively simple and reliably scorable polymorphisms and were used for segregation analysis. Sixty F2 individuals were scored for the segregation of 73 RAPD markers and 55 of these markers fit a 3:1 ratio. Segregation of eight other RAPD markers deviated significantly from a 3:1 ratio. There was no bias in the inheritance of RAPD markers regarding parental origin of the segregating RAPD markers. Linkage analysis revealed 10 linkage groups containing a total of 44 RAPD loci. Another 10 RAPD markers (7 of maternal origin) that were polymorphic between the parents did not segregate in the F2 population. One of the maternally inherited RAPD bands hybridized to chloroplast DNA. Analysis of RAPD loci by DNA hybridization indicated that mainly repeated sequences were amplified. These data indicate that RAPDs are useful genetic markers in Stylosanthes spp. and they may be suitable for genetic mapping.Key words: genetic mapping, molecular markers, polymerase chain reaction, Stylosanthes hamata, Stylosanthes scabra.

Genetic segregation of random amplified polymorphic DNA in diploid cultivated alfalfa

Genome ◽  
1992 ◽  
Vol 35 (1) ◽  
pp. 84-87 ◽  
Author(s):  
C. S. Echt ◽  
L. A. Erdahl ◽  
T. J. McCoy
Keyword(s):  
Polymerase Chain Reaction ◽  
Rapd Markers ◽  
Genome Mapping ◽  
Polymerase Chain Reaction Amplification ◽  
Random Amplified Polymorphic Dna ◽  
Rapid Development ◽  
Arbitrary Sequence ◽  
Chain Reaction ◽  
Backcross Population ◽  
Polymerase Chain

Polymerase chain reaction was used, with single 10-mer primers of arbitrary sequence, to amplify random regions of genomic DNA from a diploid cultivated alfalfa backcross population. Segregation of the random amplified polymorphic DNA (RAPD) fragments was analysed to determine if RAPD markers are suitable for use as genetic markers. Of the 19 primers tested, 13 amplified a total of 37 polymorphic fragments, of which 28 (76%) segregated as dominant Mendelian traits. RAPD markers appear useful for the rapid development of genetic information in species like alfalfa where little information currently exists or is difficult to obtain.Key words: random amplified polymorphic DNA, polymerase chain reaction amplification, genome mapping, restriction fragment length polymorphism, Medicago sativa.

Random amplified polymorphic DNA analysis in Hordeum species

Genome ◽  
1993 ◽  
Vol 36 (6) ◽  
pp. 1029-1031 ◽  
Author(s):  
Juan Manuel González ◽  
Esther Ferrer
Keyword(s):  
Polymerase Chain Reaction ◽  
Dna Polymerase ◽  
Dna Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Chain Reaction ◽  
Fragment Pattern ◽  
Polymerase Chain ◽  
Hordeum Species

Random amplified polymorphic DNA analysis was performed by applying a set of 13 arbitrary 10-mer primers to 19 Hordeum species and subspecies. High levels of variation in fragment pattern were observed both within and among species with most of the primers used. Genetic similarities between accessions and species were calculated from the fragment patterns. The resulting phenograms confirmed previous relationships among the Hordeum species.Key words: random amplified polymorphic DNA, polymerase chain reaction, polymorphism, Hordeum.

Development of a polymerase chain reaction diagnostic test for the detection of the biotrophic pathogen Plasmopara halstedii in sunflower

1999 ◽  
Vol 45 (9) ◽  
pp. 797-803 ◽  
Author(s):  
Patricia Roeckel-Drevet ◽  
Jeanne Tourvieille ◽  
Joël R Drevet ◽  
Véronique Says-Lesage ◽  
Paul Nicolas ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Helianthus Annuus ◽  
Downy Mildew ◽  
Random Amplified Polymorphic Dna ◽  
Parasitic Fungus ◽  
Sequence Information ◽  
Chain Reaction ◽  
Plasmopara Halstedii ◽  
Polymerase Chain

The obligate parasitic fungus-like organism Plasmopara halstedii (Farl.) Berl. et De Toni, is the causal agent of downy mildew disease in sunflower (Helianthus annuus). New races of this economically important parasite are regularly detected throughout the world. In addition, fungicide-resistant isolates have been reported in Europe and North America. These observations of parasite evolution, as well as the risk of propagation of the disease by infected seeds, means that it is necessary to guarantee the absence of Plasmopara halstedii in seed shipments. We report here the development of a rapid assay that can be used to detect infection by Plasmopara halstedii in plant tissues. Based on the nucleotide sequence information obtained from one cloned random amplified polymorphic DNA fragment, specific oligonucleotides were designed and used as primers for in vitro DNA amplification by polymerase chain reaction. An amplification product was detected on agarose gel stained with ethidium bromide when DNA from various Plasmopara halstedii races was tested, whereas no amplified DNA was detected when DNA from other origins was tested, including DNA from the host plant. The sensitivity of the technique was evaluated. The assay successfully reveals the presence of Plasmopara halstedii in infected sunflower plants prior to sporulation.Key words : diagnosis, polymerase chain reaction, SCAR, downy mildew, Helianthus annuus.

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