A molecular marker based linkage map of Vitis

Genetic linkage maps of Vitis (2n = 38) have been constructed from a single interspecific hybrid grape population (60 seedlings) of 'Cayuga White' × 'Aurore'. The maps were primarily based on 422 RAPD markers but also included 16 RFLP and isozyme markers. These maps had an average distance of 6.1 cM between markers and were developed using a double-pseudotestcross strategy. The 'Cayuga White' map had 214 markers covering 1196 cM and that of 'Aurore' spanned over 1477 cM with 225 markers. The 'Cayuga White' map consisted of 20 linkage groups, whereas 22 linkage groups comprised the 'Aurore' map. The number of groups reduced to 19, as in some instances two or more groups from one parent showed homology with a single group from the other parent on the basis of markers heterozygous in both parents. Each linkage group ranged in size from 14 to 135 cM in 'Aurore' and from 14 to 124 cM in 'Cayuga White'. These maps provide enough coverage of the genome to allow quantitative trait locus analysis and map-based gene cloning.Key words: grape genome, pseudotestcross, RAPD, recombination frequency, DNA content.

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Genetic Linkage Maps of the Guppy ( Poecilia reticulata ): Assignment of RAPD Markers to Multipoint Linkage Groups

Marine Biotechnology ◽

10.1007/s10126-002-0072-3 ◽

2003 ◽

Vol 5 (3) ◽

pp. 279-293 ◽

Cited By ~ 16

Author(s):

Gideon Khoo ◽

Meng Huat Lim ◽

Haridas Suresh ◽

Damien K. Y. Gan ◽

Kok Fang Lim ◽

...

Keyword(s):

Genetic Linkage ◽

Rapd Markers ◽

Poecilia Reticulata ◽

Linkage Maps ◽

Linkage Groups ◽

Multipoint Linkage ◽

Genetic Linkage Maps

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Two genetic linkage maps of mungbean using RFLP and RAPD markers

Australian Journal of Agricultural Research ◽

10.1071/ar99052 ◽

2000 ◽

Vol 51 (4) ◽

pp. 415 ◽

Cited By ~ 39

Author(s):

C. J. Lambrides ◽

R. J. Lawn ◽

I. D. Godwin ◽

J. Manners ◽

B. C. Imrie

Keyword(s):

Linkage Map ◽

Segregation Distortion ◽

Recombinant Inbred ◽

Genetic Linkage ◽

Rapd Markers ◽

Rflp Markers ◽

Linkage Maps ◽

Linkage Groups ◽

Genetic Linkage Maps ◽

Map Distance

Two genetic linkage maps of mungbean derived from the cross Berken ACC 41 are reported. The F2 map constructed from 67 individuals consisted of 110 markers (52 RFLP and 56 RAPD) that grouped into 12 linkage groups. The linked markers spanned a total map distance of 758.3 cM. A recombinant inbred (RI) population derived from the 67 F2 individuals was used for the generation of an additional linkage map. The RI map, composed entirely of RAPD markers, consisted of 115 markers in 12 linkage groups. The linked markers spanned a total map distance of 691.7 cM. Using a framework set of RFLP markers, the F2 map was compared with another F2 mungbean map constructed in Minnesota. In general, the order of these markers was consistent between maps. Segregation distortion was observed for some markers. 14.5% (16/110) of mapped F2 markers and 24% (28/115) of mapped RI markers segregated with distorted ratios. Segregation distortion occurred in each successive generation after the F2 . The regions of distortion identified in the Australian maps did not coincide with regions of the Minnesota map.

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Randomly Amplified Polymorphic DNA-based Genetic Linkage Maps of Three Apple Cultivars

Journal of the American Society for Horticultural Science ◽

10.21273/jashs.122.3.350 ◽

1997 ◽

Vol 122 (3) ◽

pp. 350-359 ◽

Cited By ~ 59

Author(s):

Patrick J. Conner ◽

Susan K. Brown ◽

Norman F. Weeden

Keyword(s):

Skin Color ◽

Genetic Linkage ◽

Average Distance ◽

Scab Resistance ◽

Linkage Maps ◽

Randomly Amplified Polymorphic Dna ◽

Linkage Groups ◽

Single Linkage ◽

Genetic Linkage Maps ◽

Using Data

Genetic linkage maps were created for three apple (Malus ×domestica Borkh.) cultivars using data from two progenies (`Wijcik McIntosh' xNY 75441-67 and `Wijcik McIntosh' xNY 75441-58). The maps consist primarily of randomly amplified polymorphic DNA (RAPD) markers, but also contain six isozyme loci and four morphological markers (Rf, fruit skin color; Vf, scab resistance; Co, columnar growth habit; Ma, malic acid). Maps were constructed using a double pseudotestcross mapping format and JoinMap mapping software. An integrated `Wijcik McIntosh' map was produced by combining marker data from both progenies into a single linkage map. Homologous linkage groups from paternal maps were paired with their counterparts in the `Wijcik McIntosh' map using locus bridges composed of markers heterozygous in both parents of a progeny. The `Wijcik McIntosh' map consists of 238 markers arranged in 19 linkage groups spanning 1206 cM. The NY 75441-67 map contains 110 markers in 16 linkage groups and the NY 75441-58 map consists of 183 markers in 18 linkage groups. The average distance between markers in the maps was ≈5.0 cM.

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Genetic linkage maps of the ground crickets Allonemobius fasciatus and Allonemobius socius using RAPD and allozyme markers

Genome ◽

10.1139/g98-080 ◽

1998 ◽

Vol 41 (6) ◽

pp. 841-847 ◽

Cited By ~ 6

Author(s):

Jiming Chu ◽

Daniel J Howard

Keyword(s):

Hybrid Zone ◽

Genetic Linkage ◽

Sex Chromosome ◽

Average Distance ◽

Linkage Maps ◽

Eastern United States ◽

Linkage Groups ◽

Genetic Linkage Maps ◽

Allonemobius Fasciatus ◽

Allonemobius Socius

The ground crickets, Allonemobius fasciatus and A. socius are sister species and form a mosaic hybrid zone in the eastern United States. In this study, we developed RAPD markers and constructed genetic linkage maps for the two species, as a first step toward mapping quantitative trait loci that control conspecific sperm precedence. The A. fasciatus map consists of 25 markers mapped to 8 linkage groups, spanning 400.1 contiguous centiMorgans (cM). The average distance between two framework markers is 23.5 cM. The A. socius map consists of 20 markers mapped to 6 linkage groups, spanning 400.5 contiguous cM. The average distance between two framework markers is 30.4 cM. A sex chromosome was identified in the A. socius map. These are the first reported genetic linkage maps for Orthoptera.Key words: Allonemobius, allozyme, cricket, hybrid zone, linkage maps, RAPD.

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Genetic linkage maps of Eucalyptus grandis and Eucalyptus urophylla using a pseudo-testcross: mapping strategy and RAPD markers.

Genetics ◽

10.1093/genetics/137.4.1121 ◽

1994 ◽

Vol 137 (4) ◽

pp. 1121-1137 ◽

Cited By ~ 27

Author(s):

D Grattapaglia ◽

R Sederoff

Keyword(s):

Genetic Linkage ◽

Rapd Markers ◽

Random Amplified Polymorphic Dna ◽

Eucalyptus Grandis ◽

Single Individual ◽

Linkage Maps ◽

Eucalyptus Urophylla ◽

Linkage Groups ◽

Mapping Strategy ◽

Genetic Linkage Maps

Abstract We have used a "two-way pseudo-testcross" mapping strategy in combination with the random amplified polymorphic DNA (RAPD) assay to construct two moderate density genetic linkage maps for species of Eucalyptus. In the cross between two heterozygous individuals many single-dose RAPD markers will be heterozygous in one parent, null in the other and therefore segregate 1:1 in their F1 progeny following a testcross configuration. Meiosis and gametic segregation in each individual can be directly and efficiently analyzed using RAPD markers. We screened 305 primers of arbitrary sequence, and selected 151 to amplify a total of 558 markers. These markers were grouped at LOD 5.0, theta = 0.25, resulting in the maternal Eucalyptus grandis map having a total of 240 markers into 14 linkage groups (1552 cM) and the paternal Eucalyptus urophylla map with 251 markers in 11 linkage groups (1101 cM) (n = 11 in Eucalyptus). Framework maps ordered with a likelihood support > or = 1000:1 were assembled covering 95% of the estimated genome size in both individuals. Characterization of genome complexity of a sample of 48 mapped random amplified polymorphic DNA (RAPD) markers indicate that 53% amplify from low copy regions. These are the first reported high coverage linkage maps for any species of Eucalyptus and among the first for any hardwood tree species. We propose the combined use of RAPD markers and the pseudo-testcross configuration as a general strategy for the construction of single individual genetic linkage maps in outbred forest trees as well as in any highly heterozygous sexually reproducing living organisms. A survey of the occurrence of RAPD markers in different individuals suggests that the pseudo-testcross/RAPD mapping strategy should also be efficient at the intraspecific level and increasingly so with crosses of genetically divergent individuals. The ability to quickly construct single-tree genetic linkage maps in any forest species opens the way for a shift from the paradigm of a species index map to the heterodox proposal of constructing several maps for individual trees of a population, therefore mitigating the problem of linkage equilibrium between marker and trait loci for the application of marker assisted strategies in tree breeding.

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A genetic linkage map for Stevia rebaudiana

Genome ◽

10.1139/g98-161 ◽

1999 ◽

Vol 42 (4) ◽

pp. 657-661 ◽

Cited By ~ 7

Author(s):

Y Yao ◽

M Ban ◽

J Brandle

Keyword(s):

Linkage Map ◽

Genetic Linkage ◽

Rapd Markers ◽

Genetic Linkage Map ◽

Average Distance ◽

Stevia Rebaudiana ◽

Linkage Groups ◽

Genome Map ◽

High Level ◽

Map Distance

To lay a foundation for molecular breeding efforts, the first genetic linkage map for Stevia rebaudiana has been constructed using segregation data from a pseudo test-cross F1 population. A total of 183 randomly amplified polymorphic DNA (RAPD) markers were analysed and assembled into 21 linkage groups covering a total distance of 1389 cM, with an average distance between markers of of 7.6 cM. The 11 largest linkage groups consisted of 4-19 loci, ranged in length from 56 to 174 cM, and accounted for 75% of the total map distance. Fifteen RAPD loci were found to be unlinked. From the 521 primers showing amplification products, 185 (35.5%) produced a total of 293 polymorphic fragments, indicating a high level of genetic diversity in stevia. Most of the RAPD markers in stevia segregated in normal Mendelian fashion.Key words: stevia, open-pollinated, genome map, RAPD.

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Linkage of random amplified polymorphic DNA, isozyme and morphological markers in grasspea (Lathyrus sativus)

The Journal of Agricultural Science ◽

10.1017/s0021859699007108 ◽

1999 ◽

Vol 133 (4) ◽

pp. 389-395 ◽

Cited By ~ 13

Author(s):

M. A. CHOWDHURY ◽

A. E. SLINKARD

Keyword(s):

Linkage Map ◽

Genetic Linkage ◽

Rapd Markers ◽

Genetic Linkage Map ◽

Average Distance ◽

Random Amplified Polymorphic Dna ◽

Lathyrus Sativus ◽

Morphological Markers ◽

Linkage Studies ◽

Linkage Groups

We constructed a genetic linkage map of grasspea (Lathyrus sativus L.; 2n = 14) from 100 F2 individuals derived from a cross between PI 426891.1.3 and PI 283564c.3.2. A total of 71 RAPD, three isozyme and one morphological markers segregated in the F2 progeny. A small fraction of markers (12%) deviated significantly from the expected Mendelian ratio (1[ratio ]2[ratio ]1 or 3[ratio ]1). Out of 75 markers, 69 (one morphological, three isozyme and 65 RAPD markers) were assigned to 14 linkage groups comprising 898 cM. The average distance between two adjacent markers was 17·2 cM. The present linkage map will serve as a reference point for further linkage studies in grasspea.

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Targetting microsatellites (SSRs) in genetic linkage maps of bread wheat

Australian Journal of Agricultural Research ◽

10.1071/ar01026 ◽

2001 ◽

Vol 52 (12) ◽

pp. 1143 ◽

Cited By ~ 2

Author(s):

M. J. Hayden ◽

S. Khatkar ◽

P. J. Sharp

Keyword(s):

Bread Wheat ◽

Ssr Markers ◽

Genetic Linkage ◽

Linkage Maps ◽

Linkage Groups ◽

Intraspecific Polymorphism ◽

Chromosomal Origin ◽

Genetic Linkage Maps ◽

Genomic Regions ◽

Simple Sequence

The construction of genetic linkage maps from intraspecific crosses of bread wheat is slow and difficult due to very limited levels of polymorphism, which hinder the assignment of linkage groups to chromosomes and leave large genomic regions without markers. Simple sequence repeats (SSRs) reveal a higher incidence of polymorphism and are more informative than any other DNA marker, and are therefore considered a marker of choice for self-pollinating crops with little intraspecific polymorphism. However, the availability of SSRs in bread wheat is still limited. In this study, selectively amplified microsatellite (SAM) analysis was used to develop informative SSR markers to assist in the construction of an intraspecific wheat map. Three markers were developed for under-represented regions in the genetic map, and 7 for unassigned linkage groups. The latter SSRs permitted the chromosomal origin of 4 unassigned linkage groups to be determined. These results demonstrate the utility of SAM analysis for the targetted development of informative SSR markers to genomic regions of interest, and assignment of linkage groups to chromosomes. Furthermore, SAM analysis facilitates the development of markers for relatively short (<11) dinucleotide repeat sequences, a class of SSRs generally inaccessible to traditional hybridisation-based methods used to develop these markers.

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Integration of the Cytogenetic and Genetic Linkage Maps of Brassica oleracea

Genetics ◽

10.1093/genetics/161.3.1225 ◽

2002 ◽

Vol 161 (3) ◽

pp. 1225-1234 ◽

Cited By ~ 5

Author(s):

Elaine C Howell ◽

Guy C Barker ◽

Gareth H Jones ◽

Michael J Kearsey ◽

Graham J King ◽

...

Keyword(s):

Linkage Map ◽

Brassica Oleracea ◽

Genetic Map ◽

Genetic Linkage ◽

Linkage Maps ◽

Linkage Groups ◽

Opposite Orientation ◽

Cytogenetic Map ◽

Specific Pcr ◽

Genetic Linkage Maps

Abstract We have assigned all nine linkage groups of a Brassica oleracea genetic map to each of the nine chromosomes of the karyotype derived from mitotic metaphase spreads of the B. oleracea var. alboglabra line A12DHd using FISH. The majority of probes were BACs, with A12DHd DNA inserts, which give clear, reliable FISH signals. We have added nine markers to the existing integrated linkage map, distributed over six linkage groups. BACs were definitively assigned to linkage map positions through development of locus-specific PCR assays. Integration of the cytogenetic and genetic linkage maps was achieved with 22 probes representing 19 loci. Four chromosomes (2, 4, 7, and 9) are in the same orientation as their respective linkage groups (O4, O7, O8, and O6) whereas four chromosomes (1, 3, 5, and 8) and linkage groups (O3, O9, O2, and O1) are in the opposite orientation. The remaining chromosome (6) is probably in the opposite orientation. The cytogenetic map is an important resource for locating probes with unknown genetic map positions and is also being used to analyze the relationships between genetic and cytogenetic maps.

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