Chikungunya virus in salivary glands of Aedes aegypti (L.): an electron microscope study

Aedes aegypti were infected with chikungunya virus by being fed on a blood–virus suspension poured over a sugar cube. The virus infection in the salivary glands was then studied with the electron microscope. In the proximal portion of the lateral lobes, 250–310 Å virus precursor particles were seen in the nucleus, in the cytoplasm, and on the membranes of cytoplasmic vesicles. Enveloped 500–580 Å virus particles with a 250–310 Å core were seen within the vesicles, in intercellular spaces, and in large numbers in the apical cavity and periductal space. In the distal portions of the lateral and median lobes precursor particles were present in the nucleus and cytoplasm, but no cytoplasmic vesicles were seen. Numerous enveloped virus particles were seen in the apical cavity and periductal space, and in the median lobe within the duct lumen as well. No evidence of virus replication was seen in the intermediate portion of the median lobe.In the distal portions, virus particles were frequently associated with a concentration of the secretory material. No other microscopically visible pathological changes were seen in the infected salivary glands.

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An Electron Microscope Study of Polyoma Virus in Hamster Kidney

The Journal of Cell Biology ◽

10.1083/jcb.7.4.753 ◽

1960 ◽

Vol 7 (4) ◽

pp. 753-760 ◽

Cited By ~ 35

Author(s):

Allan F. Howatson ◽

June D. Almeida

Keyword(s):

Electron Microscope ◽

Stromal Cells ◽

Polyoma Virus ◽

Tubular Structures ◽

Cytoplasmic Inclusions ◽

Virus Particles ◽

Large Numbers ◽

Mouse Cells ◽

Newborn Animals

Electron microscope studies were made of hamster kidneys taken at daily intervals after injection of a variant of polyoma virus into newborn animals. Particular attention was paid to the period 5 to 6 days after injection at which time the necrotizing response was at its peak and virus particles were seen in greatest numbers. The most numerous particles were about 28 mµ in diameter. They were observed mainly within nuclei of stromal cells and are similar to the particles seen in large numbers in polyoma-infected mouse cells growing in vitro. They were not observed in cells of fully developed tumors. Filamentous or tubular structures closely associated with the 28 mµ particles and probably concerned in their formation are described. Considerable quantities of viral material were contained within cytoplasmic inclusions. In some of the inclusions larger particles of diameter 60 mµ were observed. The origin of these particles and their relation to the 28 mµ particles is discussed.

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Infection by chikungunya virus modulates the expression of several proteins in Aedes aegypti salivary glands

Parasites & Vectors ◽

10.1186/1756-3305-5-264 ◽

2012 ◽

Vol 5 (1) ◽

pp. 264 ◽

Cited By ~ 29

Author(s):

Stephane Tchankouo-Nguetcheu ◽

Edouard Bourguet ◽

Pascal Lenormand ◽

Jean-Claude Rousselle ◽

Abdelkader Namane ◽

...

Keyword(s):

Aedes Aegypti ◽

Salivary Glands ◽

Chikungunya Virus

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Observations With the Electron Microscope of Myxoma Virus on Mosquito Mouthparts

Australian Journal of Biological Sciences ◽

10.1071/bi9640903 ◽

1964 ◽

Vol 17 (4) ◽

pp. 903 ◽

Cited By ~ 2

Author(s):

BK Filshie

Keyword(s):

Electron Microscope ◽

Aedes Aegypti ◽

Myxoma Virus ◽

Virus Particles ◽

Number Of Particles

The stylets of Aedes aegypti mosquitoes which have fed on rabbits infected with myxomat,osis have been examinod with the electron microscope. Virus particles were observed adhering to the mouthpa.rts. The means of attachment, the totol number of particles, and the distribution along the stylets are discussed.

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Atypical virus particles in glandular tissues from mice injected with a recombinant murine retrovirus encoding an avian V-MYC oncogene

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100159102 ◽

1990 ◽

Vol 48 (3) ◽

pp. 310-311

Author(s):

A. Beatrice Murray ◽

Jörg Schmidt ◽

Helma Becke ◽

Arne Luz

Keyword(s):

Electron Microscopy ◽

Salivary Gland ◽

Acinar Cells ◽

Lymphoblastic Lymphoma ◽

Normal Pancreas ◽

Intercellular Spaces ◽

Virus Particles ◽

Myc Oncogene ◽

Transmission Electron ◽

Large Numbers

A murine retrovirus construct encoding an avian v-myc oncogene (gift from B. Vennström EMBL, Heidelberg, F.R.G.) was injected into new born female NMRI mice. All mice developed malignant thymic lymphoblastic lymphoma within four months. Four of 9 mice also developed adenocarcinomas of the pancreas. Tissues were fixed in buffered formalin and samples from the pancreas tumours, lymph nodes, normal pancreas, liver and salivary gland were embedded in epon and examined by transmission electron microscopy.Large numbers of atypical virus-like particles were observed in all the samples from the pancreas tumours, normal pancreas and salivary gland, but only rare scattered particles in lymphomas. The pancreas tumours contained massive accumulations of particles in the intercellular spaces. In the normal pancreas, particles were preferentially located in pocketlike areas between the basal laminar and the surface of acinar cells (Fig.1), as well as in the narrow zone between adjoining acinar cells. Particles were occasionally seen budding from the cell surface.

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Maturation of chikungunya virus in salivary glands of Indonesia strains of Aedes aegypti and Aedes albopictus mosquitoes: An electron microscopic study.

Japanese Journal of Tropical Medicine and Hygiene ◽

10.2149/tmh1973.15.29 ◽

1987 ◽

Vol 15 (1) ◽

pp. 29-36

Author(s):

SOEDARTO SOEKIMAN ◽

TAKEO MATSUMURA

Keyword(s):

Aedes Aegypti ◽

Salivary Glands ◽

Microscopic Study ◽

Aedes Albopictus ◽

Electron Microscopic Study ◽

Chikungunya Virus ◽

Electron Microscopic

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Further Observations on the Virus of Epizootic Diarrhea of Infant Mice. An Electron Microscopic Study

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100060568 ◽

1967 ◽

Vol 25 ◽

pp. 110-111

Author(s):

W. G. Banfield ◽

G. Kasnic ◽

J. H. Blackwell

Keyword(s):

Electron Microscope ◽

Infected Cell ◽

Microscopic Study ◽

Intestinal Epithelium ◽

Ultrastructural Study ◽

Osmium Tetroxide ◽

Lead Citrate ◽

Electron Microscopic ◽

Virus Particles ◽

Infected Cells

An ultrastructural study of the intestinal epithelium of mice infected with the agent of epizootic diarrhea of infant mice (EDIM virus) was first performed by Adams and Kraft. We have extended their observations and have found developmental forms of the virus and associated structures not reported by them.Three-day-old NLM strain mice were infected with EDIM virus and killed 48 to 168 hours later. Specimens of bowel were fixed in glutaraldehyde, post fixed in osmium tetroxide and embedded in epon. Sections were stained with uranyl magnesium acetate followed by lead citrate and examined in an updated RCA EMU-3F electron microscope.The cells containing virus particles (infected) are at the tips of the villi and occur throughout the intestine from duodenum through colon. All developmental forms of the virus are present from 48 to 168 hours after infection. Figure 1 is of cells without virus particles and figure 2 is of an infected cell. The nucleus and cytoplasm of the infected cells appear clearer than the cells without virus particles.

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Selective Sampling and Orientation of Non-Homogeneous Tissues

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100100238 ◽

1968 ◽

Vol 26 ◽

pp. 98-99

Author(s):

C. C. Clawson ◽

L. W. Anderson ◽

R. A. Good

Keyword(s):

Electron Microscopy ◽

Electron Microscope ◽

Light Microscopy ◽

Random Sampling ◽

New Method ◽

Microscope Examination ◽

Small Areas ◽

Selective Sampling ◽

Large Numbers ◽

Specific Orientation

Investigations which require electron microscope examination of a few specific areas of non-homogeneous tissues make random sampling of small blocks an inefficient and unrewarding procedure. Therefore, several investigators have devised methods which allow obtaining sample blocks for electron microscopy from region of tissue previously identified by light microscopy of present here techniques which make possible: 1) sampling tissue for electron microscopy from selected areas previously identified by light microscopy of relatively large pieces of tissue; 2) dehydration and embedding large numbers of individually identified blocks while keeping each one separate; 3) a new method of maintaining specific orientation of blocks during embedding; 4) special light microscopic staining or fluorescent procedures and electron microscopy on immediately adjacent small areas of tissue.

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Electron Microscope Study of RNA's of Paramyxoviruses

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100094231 ◽

1972 ◽

Vol 30 ◽

pp. 196-197

Author(s):

Ruchama Baum ◽

J.T. Seto

Keyword(s):

Electron Microscopy ◽

Electron Microscope ◽

Electron Microscope Study ◽

Sendai Virus ◽

Sodium Dodecyl ◽

Rna Molecules ◽

Virus Particles ◽

Molecular Weights ◽

Length Measurements

The ribonucleic acid (RNA) of paramyxoviruses has been characterized by biochemical and physiochemical methods. However, paramyxovirus RNA molecules have not been studied by electron microscopy. The molecular weights of these single-stranded viral RNA molecules are not known as yet. Since electron microscopy has been found to be useful for the characterization of single-stranded RNA, this investigation was initiated to examine the morphology and length measurements of paramyxovirus RNA's.Sendai virus Z strain and Newcastle disease virus (NDV), Milano strain, were used. For these studies it was necessary to develop a method of extracting RNA molecules from purified virus particles. Highly purified Sendai virus was treated with pronase (300 μg/ml) at 37°C for 30 minutes and the RNA extracted by the sodium dodecyl sulfate (SDS)-phenol procedure.

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