scholarly journals An Electron Microscope Study of Polyoma Virus in Hamster Kidney

1960 ◽  
Vol 7 (4) ◽  
pp. 753-760 ◽  
Author(s):  
Allan F. Howatson ◽  
June D. Almeida
Keyword(s):  
Electron Microscope ◽  
Stromal Cells ◽  
Polyoma Virus ◽  
Tubular Structures ◽  
Cytoplasmic Inclusions ◽  
Virus Particles ◽  
Large Numbers ◽  
Mouse Cells ◽  
Newborn Animals

Electron microscope studies were made of hamster kidneys taken at daily intervals after injection of a variant of polyoma virus into newborn animals. Particular attention was paid to the period 5 to 6 days after injection at which time the necrotizing response was at its peak and virus particles were seen in greatest numbers. The most numerous particles were about 28 mµ in diameter. They were observed mainly within nuclei of stromal cells and are similar to the particles seen in large numbers in polyoma-infected mouse cells growing in vitro. They were not observed in cells of fully developed tumors. Filamentous or tubular structures closely associated with the 28 mµ particles and probably concerned in their formation are described. Considerable quantities of viral material were contained within cytoplasmic inclusions. In some of the inclusions larger particles of diameter 60 mµ were observed. The origin of these particles and their relation to the 28 mµ particles is discussed.

scholarly journals ELECTRON MICROSCOPY OF PLASMA-CELL TUMORS OF THE MOUSE

1961 ◽  
Vol 9 (2) ◽  
pp. 369-381 ◽  
Author(s):  
D. F. Parsons ◽  
M. A. Bender ◽  
E. B. Darden ◽  
Guthrie T. Pratt ◽  
D. L. Lindsley
Keyword(s):  
Electron Microscopy ◽  
Tissue Culture ◽  
Complex Structure ◽  
Granular Body ◽  
Virus Particles ◽  
Culture Cells ◽  
Large Numbers ◽  
Tumor Agent

The X5563 tumor has been grown in tissue culture. Cells similar to those of the original tumor migrated from the explant and attached to the glass walls of the culture vessels. Electron microscopy showed that large numbers of particles, similar in morphology to virus particles, were associated with these cells after 7 days of culture. The two principal types of particles found in the tumor in vivo appear to be present in vitro. Many more of these particles, however, were larger and showed a more complex structure. Whereas the particles were mainly localized inside endoplasmic reticulum or the Golgi zone in the tumors in vivo, in the tissue culture the majority of the particles were associated with the plasma membrane and were found outside of the cells. The relation of the particles to the granular body is discussed as well as a possible relation to the mammary tumor agent.

scholarly journals 315 Alstroemeria Plants Free of Alstroemeria Mosaic Potyvirus (AIMN) through in Vitro Culture Shoots and Thermotherapy

HortScience ◽  
2000 ◽  
Vol 35 (3) ◽  
pp. 446C-446
Author(s):  
Lourdes Cervantes-Dl̀az ◽  
Emma Zavaleta-Mejl̀a ◽  
Alejandra Gutièrrez-Espinosa ◽  
J. Antonio Santizo-Rincan
Keyword(s):  
In Vitro Culture ◽  
Cut Flowers ◽  
Indole Butyric Acid ◽  
Cytoplasmic Inclusions ◽  
Virus Particles ◽  
Murashige Skoog ◽  
Isopentenyl Adenine ◽  
Propagation Methods ◽  
Viral Inclusions

Alstroemeria (Alstroemeria spp.) is cultivated for cut flowers. Traditional propagation methods are by division of rhizomes from mature plants, so that viruses occurring in the crop can be multiplied and cause a decrease in the quality and production. The objective of this work was to obtain Alstroemeria cv. Rosario plants free of Alstromeria Mosaic Potyvirus (AlMV) by in vitro culture of shoots and thermotherapy. The best percentage of explants without contamination was obtained when adding the disinfectant PPM (1%) to the medium Murashige-Skoog (MS) while the best induction of buds was obtained when using explants of 1.5 cm. in length. In vitro multiplication of shoots was best in treatments with 2iP (isopentenyl adenine), BA (benzyladenine), and zeatin (4.4, 6.1, and 6.6 buds per explant, respectively). Rhizogenesis was observed in rhizomes growing in MS with 4.9 μM AIB (indole butyric acid) and 1.5 g·L-1 of sugar. Sixty-seven percent of plants growing in vitro did not react to AlMV antiserum and did not show particles and viral inclusions. Thermotherapy treatments of 45, 50, and 55 °C during different periods of time produced from 25% to 87.5% of plants that did not react to AlMV antiserum and did not show virus particles or cytoplasmic inclusions.

Electron-Microscopic Observations of Polyoma Virus-Transformed Mouse Cells Treated with Specific Immune Serum

1970 ◽  
Vol 7 (3) ◽  
pp. 711-718
Author(s):  
G. NEGRONI ◽  
RITA TILLY
Keyword(s):  
Electron Microscopy ◽  
Immune Serum ◽  
Polyoma Virus ◽  
Malignant Cells ◽  
Serum Complement ◽  
Cell Organelles ◽  
Electron Microscopic ◽  
Mouse Cells ◽  
Pig Serum

Transformed, non-malignant cells from a polyoma virus-induced mouse fibrosarcoma were treated with immune serum raised in isologous mice, and fresh guinea-pig serum (complement). Electron microscopy showed that reduction in cell viability in vitro was associated with damage to cell membranes. The extravasated cell organelles showed only minimal changes.

Chikungunya virus in salivary glands of Aedes aegypti (L.): an electron microscope study

1970 ◽  
Vol 16 (7) ◽  
pp. 581-586 ◽  
Author(s):  
H. G. Janzen ◽  
A. J. Rhodes ◽  
F. W. Doane
Keyword(s):  
Electron Microscope ◽  
Aedes Aegypti ◽  
Salivary Glands ◽  
Chikungunya Virus ◽  
Intercellular Spaces ◽  
Virus Suspension ◽  
Virus Particles ◽  
Enveloped Virus ◽  
Large Numbers ◽  
Cytoplasmic Vesicles

Aedes aegypti were infected with chikungunya virus by being fed on a blood–virus suspension poured over a sugar cube. The virus infection in the salivary glands was then studied with the electron microscope. In the proximal portion of the lateral lobes, 250–310 Å virus precursor particles were seen in the nucleus, in the cytoplasm, and on the membranes of cytoplasmic vesicles. Enveloped 500–580 Å virus particles with a 250–310 Å core were seen within the vesicles, in intercellular spaces, and in large numbers in the apical cavity and periductal space. In the distal portions of the lateral and median lobes precursor particles were present in the nucleus and cytoplasm, but no cytoplasmic vesicles were seen. Numerous enveloped virus particles were seen in the apical cavity and periductal space, and in the median lobe within the duct lumen as well. No evidence of virus replication was seen in the intermediate portion of the median lobe.In the distal portions, virus particles were frequently associated with a concentration of the secretory material. No other microscopically visible pathological changes were seen in the infected salivary glands.

scholarly journals AN ELECTRON MICROSCOPE STUDY OF THE DEVELOPMENT OF SV40 VIRUS

1963 ◽  
Vol 17 (2) ◽  
pp. 423-441 ◽  
Author(s):  
Nicole Granboulan ◽  
P. Tournier ◽  
R. Wicker ◽  
W. Bernhard
Keyword(s):  
Electron Microscope ◽  
Close Contact ◽  
Kidney Cells ◽  
Thin Sections ◽  
Sv40 Virus ◽  
Virus Particles ◽  
The Relationship ◽  
Infectious Cycle ◽  
Number Of Cells

Kidney cells, predominantly from Cercopithecus monkeys but also from baboons, were infected in vitro with the SV40 virus. The infectious cycle was studied with the electron microscope by means of thin sections of cells fixed from 3 hours up to 11 days after infection. The frequency of virus formation and various nuclear and cytoplasmic lesions in relation to the infection are described. The virus particles appear in the nucleus in close contact with the chromatin. In a small number of cells they have been observed as early as 10 to 12 hours after infection, but most often they appear 24 to 48 hours afterward. Their mean diameter is 33 mµ. They have no membrane and are frequently arranged as crystal-like structures. In addition to the appearance of virus, one observes various lesions in the nucleoplasm and particularly in the nucleolus, which shows an early hypertrophy and produces unusual, dense condensations in contact with the nucleolonema. The importance of these nucleolar lesions and the relationship between the SV40 virus and the polyoma, common wart, and Shope papilloma viruses are discussed.

Further Observations on the Virus of Epizootic Diarrhea of Infant Mice. An Electron Microscopic Study

1967 ◽  
Vol 25 ◽  
pp. 110-111
Author(s):  
W. G. Banfield ◽  
G. Kasnic ◽  
J. H. Blackwell
Keyword(s):  
Electron Microscope ◽  
Infected Cell ◽  
Microscopic Study ◽  
Intestinal Epithelium ◽  
Ultrastructural Study ◽  
Osmium Tetroxide ◽  
Lead Citrate ◽  
Electron Microscopic ◽  
Virus Particles ◽  
Infected Cells

An ultrastructural study of the intestinal epithelium of mice infected with the agent of epizootic diarrhea of infant mice (EDIM virus) was first performed by Adams and Kraft. We have extended their observations and have found developmental forms of the virus and associated structures not reported by them.Three-day-old NLM strain mice were infected with EDIM virus and killed 48 to 168 hours later. Specimens of bowel were fixed in glutaraldehyde, post fixed in osmium tetroxide and embedded in epon. Sections were stained with uranyl magnesium acetate followed by lead citrate and examined in an updated RCA EMU-3F electron microscope.The cells containing virus particles (infected) are at the tips of the villi and occur throughout the intestine from duodenum through colon. All developmental forms of the virus are present from 48 to 168 hours after infection. Figure 1 is of cells without virus particles and figure 2 is of an infected cell. The nucleus and cytoplasm of the infected cells appear clearer than the cells without virus particles.

Cytoplasmic Granules in Lymphocytes from Rats Dosed with SK&F 14336-D

1971 ◽  
Vol 29 ◽  
pp. 556-557
Author(s):  
T. G. Merrill ◽  
B. J. Payne ◽  
A. J. Tousimis
Keyword(s):  
Lipid Storage ◽  
Pokeweed Mitogen ◽  
Electron Microscopic ◽  
Cytoplasmic Granules ◽  
Storage Diseases ◽  
Tay Sachs Disease ◽  
Large Numbers ◽  
Microscopic Studies ◽  
Neurovisceral Lipidosis

Rats given SK&F 14336-D (9-[3-Dimethylamino propyl]-2-chloroacridane), a tranquilizing drug, developed an increased number of vacuolated lymphocytes as observed by light microscopy. Vacuoles in peripheral blood of rats and humans apparently are rare and are not usually reported in differential counts. Transforming agents such as phytohemagglutinin and pokeweed mitogen induce similar vacuoles in in vitro cultures of lymphocytes. These vacuoles have also been reported in some of the lipid-storage diseases of humans such as amaurotic familial idiocy, familial neurovisceral lipidosis, lipomucopolysaccharidosis and sphingomyelinosis. Electron microscopic studies of Tay-Sachs' disease and of chloroquine treated swine have demonstrated large numbers of “membranous cytoplasmic granules” in the cytoplasm of neurons, in addition to lymphocytes. The present study was undertaken with the purpose of characterizing the membranous inclusions and developing an experimental animal model which may be used for the study of lipid storage diseases.

Some Structural Features of Metaphase Chromosomes of Mice and Men

1967 ◽  
Vol 25 ◽  
pp. 190-191
Author(s):  
Godfrey C. Hoskins ◽  
Betty B. Hoskins
Keyword(s):  
Electron Microscopy ◽  
Electron Micrographs ◽  
Structural Features ◽  
Metaphase Chromosomes ◽  
The Future ◽  
Of Mice And Men ◽  
Mouse Cells ◽  
Human And Mouse

Metaphase chromosomes from human and mouse cells in vitro are isolated by micrurgy, fixed, and placed on grids for electron microscopy. Interpretations of electron micrographs by current methods indicate the following structural features.Chromosomal spindle fibrils about 200Å thick form fascicles about 600Å thick, wrapped by dense spiraling fibrils (DSF) less than 100Å thick as they near the kinomere. Such a fascicle joins the future daughter kinomere of each metaphase chromatid with those of adjacent non-homologous chromatids to either side. Thus, four fascicles (SF, 1-4) attach to each metaphase kinomere (K). It is thought that fascicles extend from the kinomere poleward, fray out to let chromosomal fibrils act as traction fibrils against polar fibrils, then regroup to join the adjacent kinomere.

Selective Sampling and Orientation of Non-Homogeneous Tissues

1968 ◽  
Vol 26 ◽  
pp. 98-99
Author(s):  
C. C. Clawson ◽  
L. W. Anderson ◽  
R. A. Good
Keyword(s):  
Electron Microscopy ◽  
Electron Microscope ◽  
Light Microscopy ◽  
Random Sampling ◽  
New Method ◽  
Microscope Examination ◽  
Small Areas ◽  
Selective Sampling ◽  
Large Numbers ◽  
Specific Orientation

Investigations which require electron microscope examination of a few specific areas of non-homogeneous tissues make random sampling of small blocks an inefficient and unrewarding procedure. Therefore, several investigators have devised methods which allow obtaining sample blocks for electron microscopy from region of tissue previously identified by light microscopy of present here techniques which make possible: 1) sampling tissue for electron microscopy from selected areas previously identified by light microscopy of relatively large pieces of tissue; 2) dehydration and embedding large numbers of individually identified blocks while keeping each one separate; 3) a new method of maintaining specific orientation of blocks during embedding; 4) special light microscopic staining or fluorescent procedures and electron microscopy on immediately adjacent small areas of tissue.

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