Halogenated compounds for the sensitive detection of clostridia by gas chromatography

1972 ◽  
Vol 18 (10) ◽  
pp. 1519-1523 ◽  
Author(s):  
Brij M. Mitruka ◽  
Martin Alexander
Keyword(s):  
Gas Chromatography ◽  
Organic Acids ◽  
Electron Capture ◽  
Clostridium Perfringens ◽  
Electron Capture Detector ◽  
Sensitive Detection ◽  
Gas Chromatograph ◽  
Halogenated Compounds ◽  
Clostridium Species

Metabolites formed by the equivalent of 230 to 3000 cells of several Clostridium species were detected by use of a gas chromatograph fitted with an electron capture detector. A variety of organic acids, alcohols, and ketones were produced by growing cultures of the 10 species tested. Compounds excreted by 300 to 3000 cells of 12 strains of Clostridium perfringens were detected, and both common and dissimilar metabolites were formed by these strains. The sensitivity for detecting six Clostridium species increased up to several thousandfold when chlorophenoxyacetates were added to the medium, the increase being most marked with 2,4-di- and 2,4,5-trichlorophenoxyacetates. The results show that cometabolism is useful in increasing the sensitivity and speed of detection of Clostridium species.

scholarly journals ANALYSIS OF FENPROPATHRIN, LAMBDA-CYHALOTHRIN, AND CHLOROTHALONIL IN POTATO AND TOMATO SAMPLES USING GAS CHROMATOGRAPHY WITH AN ELECTRON CAPTURE DETECTOR

2018 ◽  
Vol 10 (1) ◽  
pp. 70
Author(s):  
Nadia Rahmiani ◽  
Harmita . ◽  
Herman Suryadi
Keyword(s):  
Gas Chromatography ◽  
Electron Capture ◽  
Limit Of Detection ◽  
High Sensitivity ◽  
Electron Capture Detector ◽  
Gas Chromatograph ◽  
Vegetable Sample ◽  
Lambda Cyhalothrin ◽  
Limit Of Quantitation ◽  
Validation Tests

Objective: This study aimed to analyze pesticide contents in potato and tomato samples.Methods: In the present study, we determined the presence of the pesticides fenpropathrin, lambda-cyhalothrin, and chlorothalonil in conventionaland organic potatoes and tomatoes using a gas chromatograph equipped with an electron capture detector and validated the associated methods.Acetone-based extraction was performed using the Dutch mini-Luke method with minimal weights and volumes.Results: Validation tests showed a range of 70–120% and precision of ≤20%, and linearity tests on the three standard pesticides gave r values of≥0.9990 for all three pesticides. Limit of detection and limit of quantitation values showed high sensitivity, although in vegetable sample analyses,none of the three pesticides were detected.Conclusion: Our data show that the chosen method for analysis of the pesticides fenpropathrin, lambda-cyhalothrin, and chlorothalonil in potatoesand tomatoes is valid and that the marketed potatoes and tomatoes meet the SNI 7313: 2008 standard for “Maximum Limits of Pesticide Residues onAgricultural Products” and the associated Japanese standards

Gas Chromatographic Determination of N-Butyl-N-ethyl-α,α,α-trifluoro-2,6-dinitro-p-toluidine and α,α,α-Trifluoro-2,6-dinitro-N,N-dipropyl-p-toluidine in Formulations

1971 ◽  
Vol 54 (3) ◽  
pp. 711-712
Author(s):  
Martha Fuzesi
Keyword(s):  
Gas Chromatography ◽  
Quantitative Determination ◽  
Electron Capture ◽  
Chromatographic Method ◽  
Chromatographic Determination ◽  
Electron Capture Detector ◽  
Reference Solution ◽  
Gas Chromatographic Method

Abstract A gas chromatographic method is described for the quantitative determination of N-butyl-N-ethyl-α,α,α-trifluoro-2,6-dinitro-p-tolindine and α,α,α-trifluoro-2,6-dinitro-N,N-dipropyI-p-toluidine herbicides in formulations. The sample is extracted with benzene, and equal amounts of sample and reference solution in the same concentration range are analyzed by gas chromatography, using an electron capture detector and an SE-30/Diatoport S column. The method has been applied successfully to laboratory-prepared and commercial samples.

Rapid analysis of cyclamate in foods and beverages by gas chromatography-electron capture detector (GC-ECD)

2012 ◽  
Vol 134 (4) ◽  
pp. 2424-2429 ◽  
Author(s):  
Shengbing Yu ◽  
Binghui Zhu ◽  
Fen Lv ◽  
Shaoxiao Li ◽  
Weixiong Huang
Keyword(s):  
Gas Chromatography ◽  
Electron Capture ◽  
Rapid Analysis ◽  
Electron Capture Detector

ANALYSIS OF C4 EXPLOSIVE RESIDUES ON POST BLAST HAIR SAMPLES

2012 ◽  
Vol 57 (1) ◽  
Author(s):  
UMI KALTHOM AHMAD ◽  
NOOR HIDAYAT ABU BAKAR ◽  
SHAIKH ABDUL ADZIZ SHAIKH ABDULLAH
Keyword(s):  
Gas Chromatography ◽  
Electron Capture ◽  
Capillary Column ◽  
Human Head ◽  
Electron Capture Detector ◽  
Pentaerythritol Tetranitrate ◽  
High Explosives ◽  
Hair Samples

The occurrence of crimes involving high explosives in Malaysia in recent years has warrant the need for the analysis of high explosives in post blast samples, particularly residues that could be found on hairs of victims. This study reports on the detection of post blast residues of C4 explosives on human head hairs. Prior to a simulated explosion, a bundle of hair (200 strands) was tightly bound to a metal hook at each point of several pre–determined distances from the bomb seat. Hair samples containing post blast residues were extracted by ultrasonication using acetonitrile. Two analytes of interest, cyclotrimethylenetrinitramine (RDX) and pentaerythritol tetranitrate (PETN) were successfully separated using gas chromatography with electron capture detector (GC–ECD) on an HP5–MS capillary column. It was found that the amount of explosive residues decreased with increasing distance from the point of blast. At the nearest specified distance of 2.5m, the amount of PETN residues deposited on hair was much less compared to that of RDX residues.

Determination of indicator polychlorinated biphenyls (PCBs) by gas chromatography–electron capture detector

Chemosphere ◽  
2013 ◽  
Vol 93 (8) ◽  
pp. 1556-1560 ◽  
Author(s):  
Samuel Afful ◽  
Johannes A.M. Awudza ◽  
Stevester K. Twumasi ◽  
Shiloh Osae
Keyword(s):  
Gas Chromatography ◽  
Polychlorinated Biphenyls ◽  
Electron Capture ◽  
Electron Capture Detector

Microdetermination of Bioethanomethrin

1975 ◽  
Vol 58 (4) ◽  
pp. 781-784
Author(s):  
Donald A George ◽  
Leslie M Mcdonough
Keyword(s):  
Spectral Data ◽  
Electron Capture ◽  
Synthetic Pyrethroid ◽  
Pyrethroid Insecticide ◽  
Electron Capture Detector ◽  
Gas Chromatograph ◽  
Mass Spectral Data ◽  
Acid Moiety ◽  
Mass Spectral

Abstract A microanalytical method was developed for the determination of the experimental synthetic pyrethroid insecticide Bioethanomethrin ((5- benzyl-3-furyl) methyl trans-(+)-3-( cyclopentylidenemethyl)-2,2 -dimethylcyclopropanecarboxylate). After saponification, trichloroacetyl chloride is used to form an ester from the alcohol moiety, and trichloroethanol is used to form an ester from the acid moiety. Infrared and mass spectral data support the expected structures of the derivatives formed. Nanogram sensitivity is realized by utilizing a gas chromatograph with an electron capture detector.

scholarly journals Simultaneous Determination of Florfenicol and Florfenicol Amine in Fish, Shrimp, and Swine Muscle by Gas Chromatography with a Microcell Electron Capture Detector

2006 ◽  
Vol 89 (5) ◽  
pp. 1437-1442 ◽  
Author(s):  
Suxia Zhang ◽  
Fengyun Sun ◽  
Jiancheng Li ◽  
Linli Cheng ◽  
Jianzhong Shen
Keyword(s):  
Gas Chromatography ◽  
Detection Limit ◽  
Simultaneous Determination ◽  
Electron Capture ◽  
Solid Phase ◽  
Electron Capture Detector ◽  
Phase Extraction ◽  
Chromatography Method ◽  
Residue Study

Abstract A rapid and sensitive gas chromatography method was developed for the simultaneous determination of florfenicol (FF) and its metabolite florfenicol amine (FFA) in fish, shrimp, and swine muscle. The extracted samples were defatted with hexane and cleaned up by solid-phase extraction using Oasis MCX cartridges. The eluate was evaporated to dryness, and residues were derivatized and determined by gas chromatography with a microcell electron capture detector. Overall average recoveries ranged from 81.7 to 109.7% for fish, 94.1 to 103.4% for shrimp, and 71.5 to 91.4% for swine muscle. The detection limit was 0.5 ng/g for FF and 1 ng/g for FFA, respectively. The method was validated for the determination of incurred swine muscle samples in an actual residue study.

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