The phospholipids of Hansenula wingei, mating type 21

1972 ◽  
Vol 18 (10) ◽  
pp. 1569-1573
Author(s):  
J. C. Wirth ◽  
A. R. Magliulo
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Phosphatidyl Choline ◽  
Phosphatidyl Ethanolamine ◽  
Deae Cellulose ◽  
Phosphatidyl Serine ◽  
Hansenula Wingei ◽  
Layer Chromatography ◽  
Cellulose Column ◽  
Choline Phosphatidyl

The phospholipid fraction of the yeast H. wingei was studied by a combination of DEAE-cellulose column chromatography and thin-layer chromatography. Phosphatidyl choline, phosphatidyl serine, and phosphatidyl ethanolamine were identified. The absence of phosphoinositide was noted. An unknown acidic phospholipid accounts for less than 1% of the total phosphorus. A cerebroside or cerebroside-like compound was provisionally identified.

PHOSPHOLIPID COMPONENTS EXTRACTED FROM SASKATCHEWAN SOILS

1971 ◽  
Vol 51 (1) ◽  
pp. 19-22 ◽  
Author(s):  
C. G. KOWALENKO ◽  
R. B. McKERCHER
Keyword(s):  
Fatty Acid ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Phosphatidyl Choline ◽  
Phosphatidyl Ethanolamine ◽  
Total Phospholipid ◽  
Plant Residues ◽  
Hydrolysis Products ◽  
Layer Chromatography ◽  
Choline Phosphatidyl

Soil phospholipids and their hydrolysis products, examined by thin-layer chromatography and selective sprays, revealed two major components, phosphatidyl ethanolamine and phosphatidyl choline. Phosphatidyl choline represented about 40% of the total phospholipid P and phosphatidyl ethanolamine about 30%. Small quantities of lyso-phosphatidyl choline were isolated from one soil. It is suggested that a study of the fatty acid constituents of phospholipids may indicate whether their source is largely from microorganisms or plant residues.

Thin Layer Chromatographic and Infra Red Spectral Evidence for the Presence of Phosphonolipids in Cicada oni

1987 ◽  
Vol 42 (9-10) ◽  
pp. 1023-1026 ◽  
Author(s):  
Michael C. Moschidis
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Phosphatidyl Choline ◽  
Phosphatidyl Ethanolamine ◽  
Preparative Thin Layer Chromatography ◽  
Spectral Evidence ◽  
Infra Red ◽  
Layer Chromatography ◽  
Choline Phosphatidyl

Abstract The phosphonolipids from whole Cicada oni have been isolated by preparative thin-layer chromatography (TLC) in methanol-water (2:1, v/v) and have been identified by TLC, infra-red spectroscopy, phosphono phosphorus determinations and elemental microanalyses. The presence has been confirmed of the phosphono analogues of phosphatidyl choline, phosphatidyl ethanolamine, sphingomyelin and of ceramide aminoethyl phosphonate.

THE LIVER PHOSPHOLIPIDS OF THE DEVELOPING CHICK EMBRYO

1967 ◽  
Vol 45 (5) ◽  
pp. 627-639 ◽  
Author(s):  
R. C. Noble ◽  
J. H. Moore
Keyword(s):  
Fatty Acid ◽  
Phosphatidyl Choline ◽  
Phosphatidyl Ethanolamine ◽  
Phosphatidyl Serine ◽  
Chick Embryos ◽  
Ethanolamine Phosphatidyl ◽  
The Individual ◽  
Docosahexaenoic Acids ◽  
Choline Phosphatidyl ◽  
Fatty Acid Compositions

The concentrations and fatty acid compositions of the individual phospholipids in the livers of chick embryos on the 13th, 15th, 17th, 19th, and 21st days of incubation were compared with the concentrations and fatty acid compositions of the individual yolk phospholipids. The liver phospholipids contained higher proportions of phosphatidyl ethanolamine, phosphatidyl serine, and diphosphatidyl glycerol, and lower proportions of phosphatidyl choline, than did the yolk phospholipids. There was a constant increment (0.96 mg/day) of phosphatidyl choline in the liver during the period of incubation studied. The phosphatidyl choline, phosphatidyl ethanolamine, and phosphatidyl serine of the liver generally contained higher concentrations of stearic, arachidonic, and docosahexaenoic acids, and lower concentrations of palmitic and oleic acids than did these phospholipid fractions in the yolk. The fatty acid compositions of the sphingomyelin in the liver and yolk were similar. The most pronounced changes in the fatty acid composition of the liver phospholipids during embryonic development were observed in the phosphatidyl choline fraction. These changes suggested that the α-palmitoyl-β-arachidonyl phosphatidyl choline in the liver was gradually replaced by α-stearoyl-β-linoleoyl phosphatidyl choline and a-stearoyl-β-docosahexaenoyl phosphatidyl choline.

Use of DEAE-cellulose in inorganic thin-layer chromatography

1970 ◽  
Vol 47 ◽  
pp. 453-463 ◽  
Author(s):  
R. Kuroda ◽  
N. Yoshikuni ◽  
K. Kawabuchi
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Deae Cellulose ◽  
Layer Chromatography
Sign in / Sign up

Export Citation Format

Share Document