scholarly journals Separation of phosphatidyl ethanolamine, phosphatidyl serine, and other phospholipids by thin-layer chromatography

1962 ◽  
Vol 3 (4) ◽  
pp. 467-470 ◽  
Author(s):  
Vladimir P. Skipski ◽  
Robert F. Peterson ◽  
Marion Barclay
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Phosphatidyl Ethanolamine ◽  
Phosphatidyl Serine ◽  
Ethanolamine Phosphatidyl ◽  
Layer Chromatography

The phospholipids of Hansenula wingei, mating type 21

1972 ◽  
Vol 18 (10) ◽  
pp. 1569-1573
Author(s):  
J. C. Wirth ◽  
A. R. Magliulo
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Phosphatidyl Choline ◽  
Phosphatidyl Ethanolamine ◽  
Deae Cellulose ◽  
Phosphatidyl Serine ◽  
Hansenula Wingei ◽  
Layer Chromatography ◽  
Cellulose Column ◽  
Choline Phosphatidyl

The phospholipid fraction of the yeast H. wingei was studied by a combination of DEAE-cellulose column chromatography and thin-layer chromatography. Phosphatidyl choline, phosphatidyl serine, and phosphatidyl ethanolamine were identified. The absence of phosphoinositide was noted. An unknown acidic phospholipid accounts for less than 1% of the total phosphorus. A cerebroside or cerebroside-like compound was provisionally identified.

PHOSPHOLIPID COMPONENTS EXTRACTED FROM SASKATCHEWAN SOILS

1971 ◽  
Vol 51 (1) ◽  
pp. 19-22 ◽  
Author(s):  
C. G. KOWALENKO ◽  
R. B. McKERCHER
Keyword(s):  
Fatty Acid ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Phosphatidyl Choline ◽  
Phosphatidyl Ethanolamine ◽  
Total Phospholipid ◽  
Plant Residues ◽  
Hydrolysis Products ◽  
Layer Chromatography ◽  
Choline Phosphatidyl

Soil phospholipids and their hydrolysis products, examined by thin-layer chromatography and selective sprays, revealed two major components, phosphatidyl ethanolamine and phosphatidyl choline. Phosphatidyl choline represented about 40% of the total phospholipid P and phosphatidyl ethanolamine about 30%. Small quantities of lyso-phosphatidyl choline were isolated from one soil. It is suggested that a study of the fatty acid constituents of phospholipids may indicate whether their source is largely from microorganisms or plant residues.

Thin Layer Chromatographic and Infra Red Spectral Evidence for the Presence of Phosphonolipids in Cicada oni

1987 ◽  
Vol 42 (9-10) ◽  
pp. 1023-1026 ◽  
Author(s):  
Michael C. Moschidis
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Phosphatidyl Choline ◽  
Phosphatidyl Ethanolamine ◽  
Preparative Thin Layer Chromatography ◽  
Spectral Evidence ◽  
Infra Red ◽  
Layer Chromatography ◽  
Choline Phosphatidyl

Abstract The phosphonolipids from whole Cicada oni have been isolated by preparative thin-layer chromatography (TLC) in methanol-water (2:1, v/v) and have been identified by TLC, infra-red spectroscopy, phosphono phosphorus determinations and elemental microanalyses. The presence has been confirmed of the phosphono analogues of phosphatidyl choline, phosphatidyl ethanolamine, sphingomyelin and of ceramide aminoethyl phosphonate.

Thin-Layer Chromatography

1965 ◽  
Author(s):  
H. R. Bolliger ◽  
M. Brenner ◽  
H. Gänshirt ◽  
Helmut K. Mangold ◽  
H. Seiler ◽  
...  
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Layer Chromatography

THE LESS-POLAR METABOLITES PRODUCED BY INCUBATION OF TESTOSTERONE-4-14C WITH RAT AND BOVINE BRAIN

1969 ◽  
Vol 61 (4) ◽  
pp. 641-648 ◽  
Author(s):  
Leon J. Sholiton ◽  
Emile E. Werk
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Paper Chromatography ◽  
Specific Activity ◽  
Bovine Brain ◽  
Polar Fraction ◽  
End Products ◽  
Polar Metabolites ◽  
Layer Chromatography ◽  
Constant Specific Activity

ABSTRACT Rat and bovine brain have been incubated with testosterone-4-14C under standard conditions. With use of paper chromatography, the extracted metabolites were noted to fall into less-polar, iso-polar, and more polar fractions. The components of the less-polar fraction were separated by acetylation and thin-layer chromatography and the major end-products identified by recrystallization to constant specific activity or constant 3H/14C ratios. Androst-4-enedione and 5α-dihydrotestosterone were formed consistently under the conditions utilized. Trace amounts of other less-polar metabolites were noted occasionally.

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