Pseudomonas bacteriophage contains an inner body in its capsid

1984 ◽  
Vol 30 (6) ◽  
pp. 758-762 ◽  
Author(s):  
V. N. Krylov ◽  
T. A. Smirnova ◽  
I. B. Minenkova ◽  
T. G. Plotnikova ◽  
I. Z. Zhazikov ◽  
...  

Electron microscopical examination of the new virulent bacteriophage [Formula: see text], specific for Pseudomonas aeruginosa, has revealed an unusual structure in its capsid. In the center of the phage head is a cylinder of low electron density ("inner body"), surrounded by fibrous material which is packed around the inner body in a spoollike manner. The inner body itself has a springlike appearance. These structures disappear after adsorption of phage particles to bacteria. Various morphological forms, which can be interpreted as intermediate steps in [Formula: see text] DNA condensation, have been seen in ultrathin sections of [Formula: see text]-infected cells.

1976 ◽  
Vol 22 (11) ◽  
pp. 1628-1633 ◽  
Author(s):  
W. H. Van Eck

The suitability of two types of membrane filters for scanning and transmission electron-microscopical examination of chlamydospores formed from macroconidia of Fusarium solani from soil was tested. An improved method to incubate propagules in soil and to collect them free from soil particles for electron-microscopical observations is described. Best results were obtained if macroconidia were incubated in soil between two Nuclepore membrane filters. Both chlamydospore morphology and lysis, however, were affected to some extent in comparison with that on single membranes. This is probably due to a selective effect on the microflora colonizing the chlamydospores.


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