Suitability of membrane-filter techniques to study the ultrastructure of Fusarium solani in soil

1976 ◽  
Vol 22 (11) ◽  
pp. 1628-1633 ◽  
Author(s):  
W. H. Van Eck
Keyword(s):  
Fusarium Solani ◽  
Membrane Filter ◽  
Microscopical Examination ◽  
Improved Method ◽  
Selective Effect ◽  
Electron Microscopical Examination ◽  
Membrane Filters ◽  
Transmission Electron ◽  
Electron Microscopical ◽  
Nuclepore Membrane

The suitability of two types of membrane filters for scanning and transmission electron-microscopical examination of chlamydospores formed from macroconidia of Fusarium solani from soil was tested. An improved method to incubate propagules in soil and to collect them free from soil particles for electron-microscopical observations is described. Best results were obtained if macroconidia were incubated in soil between two Nuclepore membrane filters. Both chlamydospore morphology and lysis, however, were affected to some extent in comparison with that on single membranes. This is probably due to a selective effect on the microflora colonizing the chlamydospores.

Pseudomonas bacteriophage contains an inner body in its capsid

1984 ◽  
Vol 30 (6) ◽  
pp. 758-762 ◽  
Author(s):  
V. N. Krylov ◽  
T. A. Smirnova ◽  
I. B. Minenkova ◽  
T. G. Plotnikova ◽  
I. Z. Zhazikov ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Electron Density ◽  
Fibrous Material ◽  
Dna Condensation ◽  
Microscopical Examination ◽  
Unusual Structure ◽  
Electron Microscopical Examination ◽  
Infected Cells ◽  
Ultrathin Sections ◽  
Electron Microscopical

Electron microscopical examination of the new virulent bacteriophage [Formula: see text], specific for Pseudomonas aeruginosa, has revealed an unusual structure in its capsid. In the center of the phage head is a cylinder of low electron density ("inner body"), surrounded by fibrous material which is packed around the inner body in a spoollike manner. The inner body itself has a springlike appearance. These structures disappear after adsorption of phage particles to bacteria. Various morphological forms, which can be interpreted as intermediate steps in [Formula: see text] DNA condensation, have been seen in ultrathin sections of [Formula: see text]-infected cells.

Quantitative microanalysis of cristobalite by X-ray powder diffraction

1983 ◽  
Vol 16 (1) ◽  
pp. 21-23 ◽  
Author(s):  
E. Bye
Keyword(s):  
Detection Limit ◽  
Membrane Filter ◽  
Sample Holder ◽  
X Ray Diffraction ◽  
X Ray ◽  
Membrane Filters ◽  
Quantitative Microanalysis ◽  
Instrumental Drift ◽  
Nuclepore Membrane

A method for the quantitative X-ray diffraction analysis of microgram amounts of cristobalite is presented. Dust samples (1–2 mg) distributed on Nuclepore membrane filters are placed upon a silver membrane filter in a rotating sample holder. The diffraction pattern intensities of the silver filter are used to correct for absorption and for long-term instrumental drift. The detection limit is 10 μg with an accuracy of ± 10% at the 0.3 mg level.

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