DETERMINATION OF GLUCOSE IN FISH BLOOD: A MODIFICATION OF THE FOLIN–MALMROS MICROPROCEDURE

1958 ◽  
Vol 36 (11) ◽  
pp. 1121-1124 ◽  
Author(s):  
Leonard R. Murrell ◽  
Paul F. Nace
Keyword(s):  
Blood Glucose ◽  
Prussian Blue ◽  
Tungstic Acid ◽  
Sodium Lauryl Sulphate ◽  
Blood Proteins ◽  
Colorimetric Measurement ◽  
Efficient Separation ◽  
Fish Blood

A modification of the Folin–Malmros micromethod is described for the determination of fish blood glucose. A more efficient separation of blood proteins is obtained by precipitating 0.1 ml blood in 10.0 ml tungstic acid. This is followed by hot alkaline reduction of ferricyanide, and colorimetric measurement of a Prussian blue – sodium lauryl sulphate suspension. The method, which eliminates certain difficulties found in other procedures, has been successfully applied in more than a thousand analyses of blood from two species of fish (Opsanus and Ictalurus) having glucose concentrations between 15 and 250 mg per cent.

DETERMINATION OF GLUCOSE IN FISH BLOOD: A MODIFICATION OF THE FOLIN–MALMROS MICROPROCEDURE

1958 ◽  
Vol 36 (1) ◽  
pp. 1121-1124 ◽  
Author(s):  
Leonard R. Murrell ◽  
Paul F. Nace
Keyword(s):  
Blood Glucose ◽  
Prussian Blue ◽  
Tungstic Acid ◽  
Sodium Lauryl Sulphate ◽  
Blood Proteins ◽  
Colorimetric Measurement ◽  
Efficient Separation ◽  
Fish Blood

A modification of the Folin–Malmros micromethod is described for the determination of fish blood glucose. A more efficient separation of blood proteins is obtained by precipitating 0.1 ml blood in 10.0 ml tungstic acid. This is followed by hot alkaline reduction of ferricyanide, and colorimetric measurement of a Prussian blue – sodium lauryl sulphate suspension. The method, which eliminates certain difficulties found in other procedures, has been successfully applied in more than a thousand analyses of blood from two species of fish (Opsanus and Ictalurus) having glucose concentrations between 15 and 250 mg per cent.

Determination of Amphetamine, Methamphetamine, and Related Amines in Blood and Urine by Gas Chromatography with Hydrogen-Flame Ionization Detector

1970 ◽  
Vol 16 (3) ◽  
pp. 195-200 ◽  
Author(s):  
Paul Lebish ◽  
Bryan S Finkle ◽  
J W Brackett
Keyword(s):  
Tungstic Acid ◽  
Retention Data ◽  
Blood Proteins ◽  
Ionization Detector ◽  
Flame Ionization ◽  
Nonpolar Column ◽  
Hydrogen Flame ◽  
Gas Chromatographic Method ◽  
Extracting Solvent

Abstract A sensitive gas-chromatographic method suitable for clinical and forensic purposes is presented for measuring methamphetamine, amphetamine, and other phenethylamines in blood and urine. When testing blood, we include a known quantity of a homologous standard, n-propylamphetamine hydrochloride, in the tungstic-acid solution used to precipitate blood proteins; the acid filtrate is washed, alkalinized, and extracted with ether. Extracted phenethylamines are converted to the corresponding acetamides, which are then concentrated and gas-chromatographed. The method for urine is similar except the known quantity of n-propylamphetamine hydrochloride is added to the sample, the protein precipitation is omitted, and chloroform is the extracting solvent. Suitable tests are described for the more stringent confirmation of amphetamine and methamphetamine required for court testimony. Retention data obtained from both a polar and a nonpolar column packing are presented for over 50 compounds related to phenethylamine.

scholarly journals Determination of light source modules on blood glucose biomimetics using the reflectance method

2021 ◽  
Author(s):  
Bayu Prastowo ◽  
Renan Prasta Jenie ◽  
Ichsan Hardyanto ◽  
Muhammad Dahrul ◽  
Johan Iskandar ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Light Source

scholarly journals Inaccuracy in automated measurement of hematocrit and corpuscular indices in the presence of severe hyperglycemia

Blood ◽  
1981 ◽  
Vol 57 (6) ◽  
pp. 1065-1067 ◽  
Author(s):  
JA Strauchen ◽  
W Alston ◽  
J Anderson ◽  
Z Gustafson ◽  
LF Fajardo
Keyword(s):  
Blood Glucose ◽  
Mean Corpuscular Volume ◽  
Hemoglobin Concentration ◽  
Mean Corpuscular Hemoglobin ◽  
Corpuscular Volume ◽  
Automated Measurement ◽  
Mean Corpuscular Hemoglobin Concentration ◽  
Corpuscular Hemoglobin ◽  
Hyperosmolar Glucose

Abstract Because we recently observed two patients with severe diabetic hyperglycemia and spuriously elevated electronically determined hematocrit and mean corpuscular volume (MCV), we investigated the effect of hyperglycemia on two popular automated hematology systems, the Coulter S and Ortho ELT-8. Marked hyperglycemia (blood glucose 800-- 2000 mg/dl) caused consistent overestimation of the electronically determined MCV compared to that derived from a simultaneous spun microhematocrit. The resultant overestimation and underestimation, respectively, of the derived values for hematocrit and mean corpuscular hemoglobin concentration may be clinically misleading. The mechanism of MCV elevation in hyperglycemia appears to be swelling of hyperosmolar glucose “loaded” erythrocytes when diluted into “isotonic” counting medium. This effect is readily circumvented by determination of a spun microhematocrit.

Micro gasometric determination of oxygen in fish blood

1956 ◽  
Vol 48 (3) ◽  
pp. 529-532 ◽  
Author(s):  
P. F. Scholander ◽  
L. van Dam
Keyword(s):  
Fish Blood

THE MICRO-DETERMINATION OF BLOOD GLUCOSE

1963 ◽  
Vol 41 (3) ◽  
pp. 211-220 ◽  
Author(s):  
D Watson ◽  
MEK Stevenson
Keyword(s):  
Blood Glucose
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