ETHYLENE PRODUCTION FROM β-ALANINE BY AN ENZYME POWDER

1967 ◽  
Vol 45 (4) ◽  
pp. 563-571 ◽  
Author(s):  
John E. Thompson ◽  
Mary Spencer

In the investigation of a pathway for ethylene biosynthesis, intermediates arising from the metabolism of β-alanine-2-14C to ethylene by enzyme powders from wax-bean cotyledons were isolated and identified by paper and thin-layer chromatography. Quantitative determinations of the label have provided evidence for the conversion of β-alanine through malonic semialdehyde, β-hydroxypropionic acid, and acrylic acid to ethylene.

Author(s):  
H. R. Bolliger ◽  
M. Brenner ◽  
H. Gänshirt ◽  
Helmut K. Mangold ◽  
H. Seiler ◽  
...  

1969 ◽  
Vol 61 (4) ◽  
pp. 641-648 ◽  
Author(s):  
Leon J. Sholiton ◽  
Emile E. Werk

ABSTRACT Rat and bovine brain have been incubated with testosterone-4-14C under standard conditions. With use of paper chromatography, the extracted metabolites were noted to fall into less-polar, iso-polar, and more polar fractions. The components of the less-polar fraction were separated by acetylation and thin-layer chromatography and the major end-products identified by recrystallization to constant specific activity or constant 3H/14C ratios. Androst-4-enedione and 5α-dihydrotestosterone were formed consistently under the conditions utilized. Trace amounts of other less-polar metabolites were noted occasionally.


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