Secretion of bile salts by intact and isolated rat livers

1969 ◽  
Vol 47 (9) ◽  
pp. 847-854 ◽  
Author(s):  
M. T. Subbiah ◽  
A. Kuksis ◽  
Sailen Mookerjea
Keyword(s):  
Bile Acids ◽  
Chromatographic Analysis ◽  
Bile Salts ◽  
Bile Ducts ◽  
Liver Perfusion ◽  
Total Bile Acid ◽  
Bile Fistula ◽  
Rat Livers ◽  
Isolated Liver ◽  
Isolated Rat

Bile was collected from bile fistula rats and from the cannulated bile ducts of normal and 2-day choline-defîcient rats during 3 h of isolated liver perfusion. The bile acids were isolated as their taurine and glycine conjugates and the component acids analyzed. In comparison to the fistula bile of normal rats, the bile of the perfused livers of both choline-free and choline-supplemented animals showed increased conjugation of bile acids with glycine and a decreased cholate/chenodeoxycholate ratio. Although the volume of bile produced was similar for both groups of animals, the choline-deficient livers showed an average of 20% decrease in total bile acid production and a 20% decrease in cholate/chenodeoxycholate ratio, when compared to the acids from the bile of the perfused normal livers. By means of combined thin-layer and gas chromatographic analysis, all biles were shown to contain lithocholic, deoxycholic, chenodeoxycholic, hyodeoxycholic, ursodeoxycholic, cholic, and α- and β-muricholic acids as well as 3β,12α-dihydroxy- and 3α,12β-dihydroxy-cholanoic acids.

BILE ACIDS IN XENOGENEIC EX-VIVO LIVER PERFUSION: FUNCTION OF XENOPERFUSED LIVERS AND COMPATIBILITY WITH HUMAN BILE SALTS AND PORCINE LIVERS

1999 ◽  
Vol 67 (9) ◽  
pp. S595
Author(s):  
D P Foley ◽  
B R Collins ◽  
J C Magee ◽  
J L Platt ◽  
E Katz ◽  
...  
Keyword(s):  
Bile Acids ◽  
Bile Salts ◽  
Ex Vivo ◽  
Liver Perfusion ◽  
Human Bile

Effect of Bile Acids on Calcium Efflux from Isolated Rat Hepatocytes and Perfused Rat Livers

1989 ◽  
Vol 191 (2) ◽  
pp. 147-152 ◽  
Author(s):  
M. S. Anwer ◽  
J. M. Little ◽  
D. G. Oelberg ◽  
P. Zimniak ◽  
R. Lester
Keyword(s):  
Bile Acids ◽  
Rat Hepatocytes ◽  
Calcium Efflux ◽  
Isolated Rat Hepatocytes ◽  
Rat Livers ◽  
Isolated Rat

Effect of bile salt on phosphatidylcholine composition and secretion of hepatic high-density lipoproteins

1990 ◽  
Vol 259 (2) ◽  
pp. G205-G211 ◽  
Author(s):  
S. J. Robins ◽  
J. M. Fasulo ◽  
G. M. Patton
Keyword(s):  
Bile Salt ◽  
Molecular Species ◽  
Bile Salts ◽  
Low Density Lipoproteins ◽  
High Density ◽  
High Density Lipoproteins ◽  
Absolute Amount ◽  
Very Low Density Lipoproteins ◽  
Rat Livers ◽  
Isolated Rat

Bile salts are necessary for the secretion of phosphatidylcholines (PCs) in bile and result in the selective secretion of highly hydrophilic molecular species of PC that contain a 16:0 acyl group. To determine the effect of bile salt on the secretion of PCs in lipoproteins, isolated rat livers were perfused with and without taurocholate. The PC composition of very-low-density lipoproteins (VLDL), newly synthesized by the liver, precisely mirrored the composition of PCs in the whole liver and was not changed with the administration of taurocholate. In contrast, both the composition of PCs in high-density lipoproteins (HDL) and the absolute amount of newly synthesized HDL were markedly affected by the administration of taurocholate. With taurocholate the PC content of HDL was increased, HDL was enriched, like bile, in 16:0 molecular species of PC, and the amount of HDL that was recovered in the perfusate was 2.5-fold greater than without taurocholate (P less than 0.001). These findings suggest that VLDL and HDL are differently derived from within the liver, that the PCs of HDL and bile originate from the same hepatic pool or by the same mechanism, and that both the secretion of bile and HDL from the liver are susceptible to regulation by bile salt.

scholarly journals Rapid kinetic analysis of the bile-salt-dependent secretion of phospholipid, cholesterol and a plasma-membrane enzyme into bile

1984 ◽  
Vol 222 (3) ◽  
pp. 631-637 ◽  
Author(s):  
P J Lowe ◽  
S G Barnwell ◽  
R Coleman
Keyword(s):  
Plasma Membrane ◽  
Bile Salt ◽  
Kinetic Analysis ◽  
Bile Salts ◽  
Mixed Micelles ◽  
Biliary Lipid ◽  
Vesicle Traffic ◽  
Membrane Enzyme ◽  
Rat Livers ◽  
Isolated Rat

Isolated rat livers were perfused under ‘one-pass’ conditions and bile was collected at 1 min intervals. After 1 min pulse, taurocholate appeared in the collected bile within 2 min, peak output occurring 2 min later. In contrast, the increased output of phospholipids and cholesterol was slower, peak output occurring 6-11 min after the original pulse of taurocholate. These results suggest that mixed micelles cannot be formed inside the cell or during passage of bile salts through the membrane, since bile salt and lipids should then parallel each other. The bile salts must therefore be pumped into the lumen and the lipids added subsequently, due to the actions of the bile salts in the canalicular lumen. It is suggested that the biliary lipid is obtained from microdomains of biliary-type lipid in the canaliculus membrane, which are vesiculated and solubilized by the action of bile salts. It is also suggested that this biliary-type lipid is brought continuously to the membrane via vesicle traffic; this traffic is increased during increased bile-salt output, and is a process that can be inhibited by colchicine.

Ultrastructural changes in isolated rat liver perfused with cyclic heptapeptide toxins from norwegian freshwater cyanobacteria (blue-green algae)

1987 ◽  
Vol 45 ◽  
pp. 858-859
Author(s):  
A.S. Dabholkar ◽  
W.W. Carmichael ◽  
K. Berg ◽  
J. Wyman
Keyword(s):  
Ultrastructural Changes ◽  
Sprague Dawley ◽  
Isolated Rat Liver ◽  
Blue Green Algae ◽  
Sprague Dawley Rats ◽  
Cyclic Heptapeptide ◽  
Intracellular Changes ◽  
Oscillatoria Agardhii ◽  
Rat Livers ◽  
Isolated Rat

Intracellular changes in the hepatocytes of isolated rat livers perfused with cyclic heptapeptide toxins are described. The toxins used are 1) -Ala-Leu- β-methyl isoAsp-Arg-ADDA-isoGlu-mdha (M.W. 944) from Microcystis aeruginosa- Lake Akersvatn, Norway; 2) -Ala-Arg-isoAsp-Arg-ADDA-isoGlu-mdha (M.W. 1023) from Oscillatoria agardhii var. - Lake Kolbatnvatn, Norway; 3) -Ala-Arg-isoAsp-Arg-ADDA-isoGlu-dha (M.W. 1009) from Oscillatoria agardhii var. isothrix - Lake Froylandsvatn, Norway. Approximate LD intraperitoneal mouse for the toxins is 50, 500 and 1000 μg/kg respectively.Livers were removed from male Sprague Dawley rats and perfused for 15 min with a blood-free perfusate (50 ml) followed by 60 min with perfusate containing i) 25, 50, or 200 μg of M. aeruginosa toxin ii) 50, 250, 500 or 1000 μg of O. agardhii var. toxin and iii) 1000, 2000, 2500 or 5000 μg of O. agardhii var. isothrix toxin. Control livers were perfused for 75 min with the blood-free perfusate.

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