BILE ACIDS IN XENOGENEIC EX-VIVO LIVER PERFUSION: FUNCTION OF XENOPERFUSED LIVERS AND COMPATIBILITY WITH HUMAN BILE SALTS AND PORCINE LIVERS

1999 ◽  
Vol 67 (9) ◽  
pp. S595
Author(s):  
D P Foley ◽  
B R Collins ◽  
J C Magee ◽  
J L Platt ◽  
E Katz ◽  
...  
Keyword(s):  
Bile Acids ◽  
Bile Salts ◽  
Ex Vivo ◽  
Liver Perfusion ◽  
Human Bile

Secretion of bile salts by intact and isolated rat livers

1969 ◽  
Vol 47 (9) ◽  
pp. 847-854 ◽  
Author(s):  
M. T. Subbiah ◽  
A. Kuksis ◽  
Sailen Mookerjea
Keyword(s):  
Bile Acids ◽  
Chromatographic Analysis ◽  
Bile Salts ◽  
Bile Ducts ◽  
Liver Perfusion ◽  
Total Bile Acid ◽  
Bile Fistula ◽  
Rat Livers ◽  
Isolated Liver ◽  
Isolated Rat

Bile was collected from bile fistula rats and from the cannulated bile ducts of normal and 2-day choline-defîcient rats during 3 h of isolated liver perfusion. The bile acids were isolated as their taurine and glycine conjugates and the component acids analyzed. In comparison to the fistula bile of normal rats, the bile of the perfused livers of both choline-free and choline-supplemented animals showed increased conjugation of bile acids with glycine and a decreased cholate/chenodeoxycholate ratio. Although the volume of bile produced was similar for both groups of animals, the choline-deficient livers showed an average of 20% decrease in total bile acid production and a 20% decrease in cholate/chenodeoxycholate ratio, when compared to the acids from the bile of the perfused normal livers. By means of combined thin-layer and gas chromatographic analysis, all biles were shown to contain lithocholic, deoxycholic, chenodeoxycholic, hyodeoxycholic, ursodeoxycholic, cholic, and α- and β-muricholic acids as well as 3β,12α-dihydroxy- and 3α,12β-dihydroxy-cholanoic acids.

Formulation buoyancy of nanoencapsulated gliclazide using primary, conjugated and deconjugated bile acids

2019 ◽  
Vol 10 (9) ◽  
pp. 573-583
Author(s):  
Sangeetha Mathavan ◽  
Corina M Ionescu ◽  
Bozica Kovacevic ◽  
Momir Mikov ◽  
Svetlana Golocorbin-Kon ◽  
...  
Keyword(s):  
Bile Acids ◽  
Sodium Alginate ◽  
Ex Vivo ◽  
Permeation Enhancers ◽  
Human Bile ◽  
Potential Applications ◽  
Specific Uptake ◽  
The Stability

Aim: Recent studies suggest potential applications of endogenously produced human bile acids as formulation-excipient and drug tissue permeation enhancers in Type 1 diabetes. We aimed to examine the stability, tissue permeation and ex vivo muscle-cell effects of microencapsulated gliclazide (G) incorporated with a primary (chenodeoxycholic acid [CDCA]), a secondary (ursodeoxycholic acid [UDCA]) or a tertiary (taurocholic acid [TCA]) bile acid. Materials & methods: Four formulations made of sodium alginate, CDCA, UDCA and TCA were examined for buoyancy, tissue-enhancing effects ( in vivo) and local ( ex vivo) viability effects. Results & conclusion: CDCA, UDCA and TCA improved buoyancy and cell viability but not tissue-specific uptake. G-TCA-sodium alginate microcapsules exerted hypoglycemic effects, suggesting significant improvement of G gut-uptake by TCA, possibly via improving buoyancy.

scholarly journals Pharmacological and Advanced Cell Respiration Effects, Enhanced by Toxic Human-Bile Nano-Pharmaceuticals of Probucol Cell-Targeting Formulations

Pharmaceutics ◽  
2020 ◽  
Vol 12 (8) ◽  
pp. 708
Author(s):  
Susbin Raj Wagle ◽  
Bozica Kovacevic ◽  
Daniel Walker ◽  
Corina Mihaela Ionescu ◽  
Melissa Jones ◽  
...  
Keyword(s):  
Drug Release ◽  
Bile Acids ◽  
Oral Delivery ◽  
Oral Absorption ◽  
Lithocholic Acid ◽  
Ex Vivo ◽  
Biological Effects ◽  
Drug Formulation ◽  
Human Bile

Bile acids have recently been studied for potential applications as formulation excipients and enhancers for drug release; however, some bile acids are not suitable for this application. Unconjugated lithocholic acid (ULCA) has recently shown drug formulation-stabilizing and anti-inflammatory effects. Lipophilic drugs have poor gut absorption after an oral dose, which necessitates the administration of high doses and causes subsequent side effects. Probucol (PB) is a highly lipophilic drug with poor oral absorption that resulted in restrictions on its clinical prescribing. Hence, this study aimed to design new delivery systems for PB using ULCA-based matrices and to test drug formulation, release, temperature, and biological effects. ULCA-based matrices were formulated for PB oral delivery by applying the jet-flow microencapsulation technique using sodium alginate as a polymer. ULCA addition to new PB matrices improved the microcapsule’s stability, drug release in vitro (formulation study), and showed a promising effect in ex vivo study (p < 0.05), suggesting that ULCA can optimize the oral delivery of PB and support its potential application in diabetes treatment.

BILE ACIDS IN XENOGENEIC EX-VIVO LIVER PERFUSION

2000 ◽  
Vol 69 (2) ◽  
pp. 242 ◽  
Author(s):  
David P. Foley ◽  
Bradley R. Collins ◽  
John C. Magee ◽  
Jeffrey L. Platt ◽  
Elizer Katz ◽  
...  
Keyword(s):  
Bile Acids ◽  
Ex Vivo ◽  
Liver Perfusion

scholarly journals Treatment of acute hepatic failure and encephalopathy with extracorporeal ex vivo pig-liver perfusion in the critical care unit

Critical Care ◽  
10.1186/cc560 ◽  
1999 ◽  
Vol 3 (Suppl 1) ◽  
pp. P187
Author(s):  
MC DaSilva ◽  
M Gupta ◽  
MJ Holman ◽  
HG Yang ◽  
RL Conter ◽  
...  
Keyword(s):  
Critical Care ◽  
Hepatic Failure ◽  
Critical Care Unit ◽  
Ex Vivo ◽  
Liver Perfusion ◽  
Acute Hepatic Failure ◽  
Pig Liver

scholarly journals Bakteri Asam Laktat Lactobacillus Plantarum C410LI dan Lactobacillus Rossiae LS6 yang Diisolasi dari Lemea Rejang terhadap Suhu, pH dan Garam Empedu Berpotensi sebagai Prebiotik

2018 ◽  
Vol 6 (1) ◽  
pp. 49-58
Author(s):  
Yenni Okfrianti ◽  
Darwis Darwis ◽  
Ayu Pravita
Keyword(s):  
Experimental Study ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Bile Acids ◽  
Bile Salts ◽  
Salt Resistance ◽  
Low Ph ◽  
Traditional Food ◽  
Temperature Resistance ◽  
Bile Acids And Salts

Based on previous research it was found that lemea (traditional food rejang) was proven to contain 2 types of lactic acid bacteria (BAL) namely L.aplantarum C410L1 and L. crossiae LS6 which could be probiotic and beneficial for health. The development of lemea as a potential probiotic must be proven its resistance to bile acids and salts as an indication of being able to survive in the gastrointestinal tract. This study aims to determine the resistance of BAL isolated from lemea against low pH, bile acids, and temperature. This research is an experimental study with all research units controlled. Analysis of BAL resistance to high temperatures, low pH, and bile salts was carried out in the Bengkulu Polytechnic Health Polytechnic laboratory. The total BAL colonies increased at 49 ° C and decreased at 64 ° C. The increase in the total number of BAL colonies within 0-30 hours occurred at pH 5 and pH 6. There was no increase or decrease in the total number of BAL colonies in salts 0.30%, 0.60%, and 0.90%. The diisolate lactic acid (BAL) bacteria from lemea have a temperature resistance of 42 ° C to 64 ° C, pH 2 to pH 7, have a salt resistance concentration of 0.30% to 0.90%. Lactic acid bacteria (BAL) which are diisolate from lemea have the potential as probiotics.

scholarly journals Bile Acids Activate NLRP3 Inflammasome, Promoting Murine Liver Inflammation or Fibrosis in a Cell Type-Specific Manner

Cells ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 2618
Author(s):  
Theresa Maria Holtmann ◽  
Maria Eugenia Inzaugarat ◽  
Jana Knorr ◽  
Lukas Geisler ◽  
Marten Schulz ◽  
...  
Keyword(s):  
Bile Acids ◽  
Liver Damage ◽  
Nlrp3 Inflammasome ◽  
Lithocholic Acid ◽  
Ex Vivo ◽  
Cell Types ◽  
Inflammasome Activation ◽  
Inflammatory Phenotype ◽  
Murine Liver ◽  
A Cell

Bile acids (BA) as important signaling molecules are considered crucial in development of cholestatic liver injury, but there is limited understanding on the involved cell types and signaling pathways. The aim of this study was to evaluate the inflammatory and fibrotic potential of key BA and the role of distinct liver cell subsets focusing on the NLRP3 inflammasome. C57BL/6 wild-type (WT) and Nlrp3−/− mice were fed with a diet supplemented with cholic (CA), deoxycholic (DCA) or lithocholic acid (LCA) for 7 days. Additionally, primary hepatocytes, Kupffer cells (KC) and hepatic stellate cells (HSC) from WT and Nlrp3−/− mice were stimulated with aforementioned BA ex vivo. LCA feeding led to strong liver damage and activation of NLRP3 inflammasome. Ex vivo KC were the most affected cells by LCA, resulting in a pro-inflammatory phenotype. Liver damage and primary KC activation was both ameliorated in Nlrp3-deficient mice or cells. DCA feeding induced fibrotic alterations. Primary HSC upregulated the NLRP3 inflammasome and early fibrotic markers when stimulated with DCA, but not LCA. Pro-fibrogenic signals in liver and primary HSC were attenuated in Nlrp3−/− mice or cells. The data shows that distinct BA induce NLRP3 inflammasome activation in HSC or KC, promoting fibrosis or inflammation.

Successful ex vivo liver perfusion system for hepatic failure pending liver regeneration or liver transplantation

2001 ◽  
Vol 33 (1-2) ◽  
pp. 1962-1964 ◽  
Author(s):  
G.M Abouna ◽  
P Ganguly ◽  
S Jabur ◽  
W Tweed ◽  
H Hamdy ◽  
...  
Keyword(s):  
Liver Transplantation ◽  
Liver Regeneration ◽  
Hepatic Failure ◽  
Ex Vivo ◽  
Liver Perfusion ◽  
Perfusion System

Three probiotic strains exert different effects on plasma bile acid profiles in healthy obese adults: randomised, double-blind placebo-controlled crossover study

2019 ◽  
Vol 10 (5) ◽  
pp. 497-509 ◽  
Author(s):  
T. Culpepper ◽  
C.C. Rowe ◽  
C.T. Rusch ◽  
A.M. Burns ◽  
A.P. Federico ◽  
...  
Keyword(s):  
Bile Acid ◽  
Glucose Metabolism ◽  
Crossover Study ◽  
Bile Acids ◽  
Disease Risk ◽  
Cardiovascular Disease Risk ◽  
Bile Acid Metabolism ◽  
Double Blind ◽  
Human Bile ◽  
Probiotic Strains

Microbial metabolism in the gut may alter human bile acid metabolism in a way that beneficially affects lipid homeostasis and therefore cardiovascular disease risk. Deconjugation of bile acids by microbes is thought to be key to this mechanism but has yet to be characterised in blood and stool while observing lipid markers. The aim of this study was to determine the effect of 3 different probiotic strains on plasma and stool bile acids in the context of lipid and glucose metabolism. In this 18-week, randomised, double-blind crossover study, healthy adults (53±8 years) with a high waist circumference underwent a 1-week pre-baseline period and were then randomised to receive 1 capsule/day of Bacillus subtilis R0179 (2.5×109 cfu/capsule; n=39), Lactobacillus plantarum HA-119 (5×109 cfu/capsule; n=38), Bifidobacterium animalis subsp. lactis B94 (5×109 cfu/capsule; n=37) or placebo for 6 weeks. Following a 3-week washout and second pre-baseline week, participants were crossed to the other intervention for 6 weeks followed by a 1-week post-intervention period. Blood and stool samples were collected at the beginning and end of each intervention to measure bile acids, serum lipid profiles, and glucose and insulin levels. Data from the placebo intervention were combined for all participants for analyses. In obese participants, the difference (final-baseline) in the sum of deconjugated plasma bile acids was greater with consumption of B. subtilis (691±378 nmol/l, P=0.01) and B. lactis (380±165 nmol/l, P=0.04) than with placebo (98±176 nmol/l, n=57). No significant differences were observed for any probiotics for stool bile acids, serum lipids, blood glucose or insulin. These data suggest that B. subtilis and B. lactis had no effect on glucose metabolism or serum cholesterol but increased deconjugated plasma bile acids in obese individuals. Additional studies should be conducted to confirm these findings and explore potential mechanisms. This trial was registered at clinicaltrials.gov as NCT01879098.

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