Identification and Distribution of p-Tyramine in the Rat

1974 ◽  
Vol 52 (5) ◽  
pp. 366-373 ◽  
Author(s):  
S. R. Philips ◽  
D. A. Durden ◽  
A. A. Boulton
Keyword(s):  
Standard Deviation ◽  
Ion Exchange ◽  
Wistar Rats ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Mass Spectrometric ◽  
Ion Current ◽  
Mammalian Tissues ◽  
Male Wistar Rats ◽  
The Brain

A procedure for the quantitative evaluation of p-tyramine in mammalian tissues is described. It involves isolation of the amine by ion-exchange chromatography, followed by conversion to the dansyl derivative, chromatographic separation, and quantitation by the mass spectrometric integrated ion current technique using an isotopically labelled internal standard.The concentrations of p-tyramine in some tissues of male Wistar rats were (mean ± standard deviation (ng/g)): brain 2.0 ± 0.9, heart 3.4 ± 1.4, kidney 32.7 ± 13.1, liver 1.5 ± 0.5, lung 3.0 ± 1.2, and spleen 3.4 ± 1.2. In the brain, hypothalamus contained 11.3 ± 3.7, cerebellum 2.3 ± 1.7, stem 2.2 ± 1.0, caudate nucleus 19.2 ± 2.5, and the 'rest' 1.6 ± 0.4 ng/g, respectively.

Identification and Distribution of m-Tyramine in the Rat

1975 ◽  
Vol 53 (1) ◽  
pp. 65-69 ◽  
Author(s):  
S. R. Philips ◽  
B. A. Davis ◽  
D. A. Durden ◽  
Alan A. Boulton
Keyword(s):  
Ion Exchange ◽  
Chromatographic Separation ◽  
Wistar Rats ◽  
Internal Standard ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Mass Spectrometric ◽  
Ion Current ◽  
Mammalian Tissues ◽  
Male Wistar Rats

A procedure for the quantitative evaluation of m-tyramine in mammalian tissues is described. It involves isolation of the amine by ion-exchange chromatography, followed by conversion to the dansyl derivative, chromatographic separation, and quantitation by the mass spectrometric integrated ion current technique using an isotopically labelled internal standard. The concentrations of m-tyramine in some tissues of male Wistar rats were (mean ± S.D., nanograms per gram): brain 0.32 ± 0.03, heart 0.44 ± 0.13. kidney 12.6 ± 3.4, liver 0.27 ± 0.04, lung 0.33 ± 0.11, spleen 0.25 ± 0.07, and blood 0.15 ± 0.04.

Identification and Distribution of β-Phenylethylamine in the Rat

1973 ◽  
Vol 51 (7) ◽  
pp. 995-1002 ◽  
Author(s):  
D. A. Durden ◽  
S. R. Philips ◽  
Alan A. Boulton
Keyword(s):  
Wistar Rats ◽  
Internal Standard ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Mass Spectrometric ◽  
Ion Current ◽  
Mammalian Tissues ◽  
Male Wistar Rats ◽  
Dansyl Derivatives ◽  
The Brain

A procedure for the quantitative evaluation of some amines present in mammalian tissues has been developed. It includes isolation of the amines by ion exchange chromatography followed by conversion to dansyl derivatives, chromatographic separation, and quantitation by the mass spectrometric integrated ion current technique. The use of an isotopically labelled internal standard improves the precision and sensitivity of the analysis.The concentrations of β-phenylethylamine in some tissues of male Wistar rats were (ng/g); brain 1.8 ± 0.4, heart 5.7 ± 3.1, kidney 20.5 ± 2.2, liver 2.0 ± 0.7, lung 4.0 ± 1.4, and spleen 4.7 ± 2.7. In the brain the hypothalamus contained 25.3 ± 5.0, the cerebellum 3.4 ± 0.5, the stem 2.2 ± 0.9, the caudate nucleus 8.0 ± 0.3, and the 'rest' 1.1 ± 0.2 ng/g, respectively.

Identification and Distribution of Tryptamine in the Rat

1974 ◽  
Vol 52 (6) ◽  
pp. 447-451 ◽  
Author(s):  
S. R. Philips ◽  
D. A. Burden ◽  
A. A. Boulton
Keyword(s):  
Wistar Rats ◽  
Quantitative Measurement ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Ion Current ◽  
Mean Deviation ◽  
Mammalian Tissues ◽  
Male Wistar Rats ◽  
Layer Chromatography ◽  
The Brain

A procedure for the quantitative measurement of tryptamine in mammalian tissues is described. The amine is isolated by ion-exchange chromatography, converted to its dansyl derivative, further purified by thin-layer chromatography, and quantitated by the mass-spectrometric integrated ion current technique using an isotopically labelled internal standard.The concentrations of tryptamine in some tissues of male Wistar rats were (ng/g ± S.D.): brain 0.50 ± 0.07, heart 0.62 ± 0.10, kidney 8.04 ± 2.10, liver 0.73 ± 0.07, lung 0.54 ± 0.18, and spleen 0.43 ± 0.14. In the brain, the hypothalamus contained 0.94 ± 0.22, the cerebellum 0.27 ± 0.02, the stem 0.24 ± 0.06, the caudate nucleus 2.93 ± 1.14, and the "rest" 0.32 ± 0.05 ng/g (mean ± mean deviation).

scholarly journals Ion Exchange Chromatography and Mass Spectrometric Methods for Analysis of Cadmium-Phytochelatin (II) Complexes

2013 ◽  
Vol 10 (4) ◽  
pp. 1304-1311 ◽  
Author(s):  
Miguel Rodrigo ◽  
Natalia Cernei ◽  
Marketa Kominkova ◽  
Ondrej Zitka ◽  
Miroslava Beklova ◽  
...  
Keyword(s):  
Ion Exchange ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Mass Spectrometric

scholarly journals A Sulfuryl Group Transfer Strategy to Selectively Prepare Sulfated Steroids and Isotopically Labelled Derivatives

2021 ◽  
Vol 8 ◽  
Author(s):  
Jaber A. Alshehri ◽  
Daniel M. Gill ◽  
Alan M. Jones
Keyword(s):  
Ion Exchange ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Mass Spectrometric ◽  
Sodium Salts ◽  
Group Transfer ◽  
Biologically Relevant ◽  
Biological Studies ◽  
Sulfated Steroids ◽  
Phenol Moiety

The treatment of common steroids: estrone, estradiol, cortisol, and pregnenolone with tributylsulfoammonium betaine (TBSAB) provides a convenient chemoselective conversion of the steroids alcohol/phenol moiety to the corresponding steroidal organosulfate. An important feature of the disclosed methodology is the millimolar scale of the reaction, and the isolation of the corresponding steroid sulfates as their biologically relevant sodium salts without the need for ion-exchange chromatography. The scope of the method was further explored in the estradiol and pregnanediol steroid systems with the bis-sulfated derivatives. Ultimately, a method to install an isotopic label, deuterium (2H) combined with estrone sulfation is a valuable tool for its mass-spectrometric quantification in biological studies.

Effective Transduction of Brain Neurons with Lentiviral Vectors Purified by Ion-Exchange Chromatography

2020 ◽  
Vol 36 (5) ◽  
pp. 89-97
Author(s):  
D.A. Lanshakov
Keyword(s):  
Gene Therapy ◽  
Ion Exchange ◽  
High Titer ◽  
Lentiviral Vectors ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
In Vivo Studies ◽  
Modern Biology ◽  
The Brain

The development of methods for purification viral vectors for gene therapy is one of the most important and urgent problems of modern biology and medicine. Recently, drugs that carry cerebral neurotrophic factors, such as BDNF, have become increasingly popular. However, viral drugs for gene therapy should meet certain requirements, including high titer and applicability for in vivo studies. At the same time, the creation of such vectors requires cost-effective, inexpensive and affordable methods for standard laboratories. This study compares various methods for purification of lentiviral vectors encoding the brain neurotrophic factor, BDNF. The highest titer (1.12 ∙ 109/mL) was obtained via PEG 6 000 precipitation followed by anion-exchange chromatography on two columns of sorbents containing quaternary ammonium groups. Abnormal aggregates of transduced neurons were detected after lentiviruses purified only by PEG precipitation were injected into the brain of a newborn rat. This fact confirms the necessity of the proposed additional chromatographic purification stage. lentivirus, BDNF, ion exchange chromatography, gene therapy, PEG This work was financially supported by budgetary funding project no. 0259-2019-0003-C-01.

Studies on an Inhibitor of Plasminogen Activation in Human Serum

1973 ◽  
Vol 30 (02) ◽  
pp. 414-424 ◽  
Author(s):  
Ulla Hedner
Keyword(s):  
Ion Exchange ◽  
Human Serum ◽  
Antithrombin Iii ◽  
Gel Filtration ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Specific Adsorption ◽  
Partial Purification ◽  
Plasminogen Activation ◽  
Preparative Electrophoresis

SummaryA procedure is described for partial purification of an inhibitor of the activation of plasminogen by urokinase and streptokinase. The method involves specific adsorption of contammants, ion-exchange chromatography on DEAE-Sephadex, gel filtration on Sephadex G-200 and preparative electrophoresis. The inhibitor fraction contained no antiplasmin, no plasminogen, no α1-antitrypsin, no antithrombin-III and was shown not to be α2 M or inter-α-inhibitor. It contained traces of prothrombin and cerulo-plasmin. An antiserum against the inhibitor fraction capable of neutralising the inhibitor in serum was raised in rabbits.

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