Cell-free synthesis of cod preproinsulin
Keyword(s):
Poly(A)-containing ribonucleic acid from cod islet greatly stimulated the incorporation of radioactive amino acids into proteins when assayed in a wheat germ translation system. The translation products were examined by specific immunoprecipitation with guinea pig anti cod insulin antibodies and by extraction with acid–ethanol. These measurements revealed at least a fivefold increase in incorporation of labelled amino acid over the nonprogrammed system. Sodium dodecyl sulfate disc gel electrophoresis and gel filtration chromatography showed a product of molecular weight 12 500, a size considerably larger than cod proinsulin (9000). It is concluded that cod proinsulin is synthesized via a larger precursor, preproinsulin.
1987 ◽
Vol 166
(2)
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pp. 235-245
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1998 ◽
Vol 180
(4)
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pp. 945-949
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1992 ◽
Vol 68
(05)
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pp. 534-538
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1984 ◽
Vol 62
(5)
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pp. 276-279
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Keyword(s):
1978 ◽
Vol 79
(1)
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pp. 132-137
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