Structural characterization of the O-polysaccharide of the lipopolysaccharide produced by Salmonella milwaukee O:43 (group U) which possesses human blood group B activity

1992 ◽  
Vol 70 (1) ◽  
pp. 49-55 ◽  
Author(s):  
Malcolm B. Perry ◽  
Leann L. MacLean
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Blood Group ◽  
Human Blood ◽  
Periodate Oxidation ◽  
Composition Analysis ◽  
Human Blood Group ◽  
O 43 ◽  
Group B ◽  
Nuclear Magnetic

The O-polysaccharide of the lipopolysaccharide produced by Salmonella milwaukee O:43 (group U) was shown by composition analysis, methylation, periodate oxidation and 1H and 13C nuclear magnetic resonance spectroscopic analytical methods to be a polymer of branched pentasaccharide repeating units having the structure:[Formula: see text]The blood-group activity of the O-polysaccharide was established by its serological reactivity with a specific monoclonal antibody to human blood group B, using passive hemaglutination and ELISA assays, indicating the common antigenic epitope to be a nonreducing terminal trisaccharide unit composed of L-Fucp and D-Galp (1:2) residues.Key words: Salmonella milwaukee, lipopolysaccharide, blood group B, polysaccharide, nuclear magnetic resonance.

scholarly journals A Single Point Mutation Reverses the Donor Specificity of Human Blood Group B-synthesizing Galactosyltransferase

2003 ◽  
Vol 278 (14) ◽  
pp. 12403-12405 ◽  
Author(s):  
Sandra L. Marcus ◽  
Robert Polakowski ◽  
Nina O. L. Seto ◽  
Eeva Leinala ◽  
Svetlana Borisova ◽  
...  
Keyword(s):  
Point Mutation ◽  
Blood Group ◽  
Human Blood ◽  
Single Point ◽  
Single Point Mutation ◽  
Human Blood Group ◽  
Group B

scholarly journals A sensitive on‐chip probe–based portable nuclear magnetic resonance for detecting low parasitaemia plasmodium falciparum in human blood

2020 ◽  
Vol 3 (4) ◽  
Author(s):  
Manish Gupta ◽  
Kundan Singh ◽  
Daya Krishan Lobiyal ◽  
Cholakka Parambath Safvan ◽  
Bhuban Kumar Sahu ◽  
...  
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Plasmodium Falciparum ◽  
Magnetic Resonance ◽  
Human Blood ◽  
On Chip ◽  
Nuclear Magnetic

Structural analysis of the three lipopolysaccharides produced by Salmonella madelia (1,6,14,25)

1989 ◽  
Vol 67 (2-3) ◽  
pp. 78-85 ◽  
Author(s):  
José L. Di Fabio ◽  
Jean-Robert Brisson ◽  
Malcolm B. Perry
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Acetic Acid ◽  
Magnetic Resonance ◽  
Nitrous Acid ◽  
Periodate Oxidation ◽  
Ps Ii ◽  
Polysaccharide Fraction ◽  
Ps I ◽  
Hydrolysis Of ◽  
Nuclear Magnetic

Salmonella madelia reported to express the O-antigenic factors 1, 6, 14, and 25, defined in the Kauffmann–White classification system, was found to produce three different homogeneous lipopolysaccharides, which differed in having three structurally distinct O-polysaccharide components. The O-polysaccharide fraction obtained by mild acetic acid hydrolysis of the S. madelia lipopolysaccharide was analyzed by chemical composition, nitrous acid deamination, periodate oxidation, methylation, and 1H and 13C nuclear magnetic resonance methods and was demonstrated to be composed of three polysaccharides, PS(I), PS(II), and PS(III), which had the structures of repeating oligosaccharide units:[Formula: see text]Key words: Salmonella madelia, lipopolysaccharide, structure, analysis, nuclear magnetic resonance.

scholarly journals Structural Effects of Naturally Occurring Human Blood Group B Galactosyltransferase Mutations Adjacent to the DXD Motif

2007 ◽  
Vol 282 (13) ◽  
pp. 9564-9570 ◽  
Author(s):  
Mattias Persson ◽  
James A. Letts ◽  
Bahram Hosseini-Maaf ◽  
Svetlana N. Borisova ◽  
Monica M. Palcic ◽  
...  
Keyword(s):  
Blood Group ◽  
Human Blood ◽  
Structural Effects ◽  
Human Blood Group ◽  
Naturally Occurring ◽  
Group B

NMR-based exploration of the acceptor binding site of human blood group B galactosyltransferase with molecular fragments

2010 ◽  
Vol 27 (3) ◽  
pp. 349-358 ◽  
Author(s):  
Christoph Rademacher ◽  
Jens Landström ◽  
Nora Sindhuwinata ◽  
Monica M. Palcic ◽  
Göran Widmalm ◽  
...  
Keyword(s):  
Binding Site ◽  
Blood Group ◽  
Human Blood ◽  
Molecular Fragments ◽  
Human Blood Group ◽  
Group B
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