Characterization of the anti-cancer-cell parasporal proteins of aBacillus thuringiensisisolate

2000 ◽  
Vol 46 (10) ◽  
pp. 913-919 ◽  
Author(s):  
Satoko Yamashita ◽  
Tetsuyuki Akao ◽  
Eiichi Mizuki ◽  
Hiroyuki Saitoh ◽  
Kazuhiko Higuchi ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Cancer Cell ◽  
Human Cancer ◽  
Proteolytic Processing ◽  
Proteinase K ◽  
Major Protein ◽  
Human Cancer Cell ◽  
Soil Isolate ◽  
Anti Cancer

An unusual activity, associated with non-insecticidal and non-haemolytic parasporal inclusion proteins of a Bacillus thuringiensis soil isolate, designated 89-T-26-17, was characterized. The parasporal inclusion of this isolate was bipyramidal, rounded at both ends, containing proteins of 180, 150, 120, 100, and 88 kDa. No homologies with the Cry and Cyt proteins of B. thuringiensis were detected based on N-terminal sequences. Proteolytic processing of the inclusion proteins by proteinase K, trypsin, and chymotrypsin produced a major protein of 64 kDa exhibiting cytocidal activity against human leukaemic T cells and uterus cervix cancer (HeLa) cells. The protease-activated proteins showed no cytotoxicity to normal T cells.Key words: Bacillus thuringiensis parasporal inclusion, non-insecticidal, non-haemolytic, cytocidal activity, human cancer cell.

Occurrence of parasporin-producingBacillus thuringiensisin Vietnam

2006 ◽  
Vol 52 (4) ◽  
pp. 365-372 ◽  
Author(s):  
Koichi Yasutake ◽  
Ngo Dinh Binh ◽  
Kumiko Kagoshima ◽  
Akiko Uemori ◽  
Akira Ohgushi ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Cancer Cell ◽  
Human Cancer ◽  
Cell Killing ◽  
Nucleotide Sequence Analysis ◽  
Human Cancer Cells ◽  
Soil Isolate ◽  
Mosquitocidal Activity ◽  
Anti Cancer ◽  
Larvicidal Activities

A total of 63 Bacillus thuringiensis isolates were recovered from urban soils of Hanoi, Vietnam. Of these, 34 were identified to 12 H serogroups. None of the isolates showed larvicidal activities against three lepidopterous insects. Three isolates belonging to the two serovars, colmeri (H21) and konkukian (H34), were highly toxic to larvae of the mosquito Aedes aegypti. Parasporal inclusion proteins of four isolates exhibited cytocidal activities against HeLa cells. Immunologically, proteins of four isolates were closely related to parasporin-1 (Cry31Aa), a parasporal protein that preferentially kills human cancer cells. Haemolytic activities were associated with parasporal proteins of the three mosquitocidal isolates but not with those of the four cancer-cell-killing isolates. PCR experiments and nucleotide sequence analysis revealed that the genes of four anti-cancer isolates are closely related to the gene parasporin-1 (cry31Aa) but are dissimilar to those of the three other existing parasporins. Our results suggest that the soil of northern Vietnam is a good reservoir of parasporin-producing B. thuringiensis.Key words: Bacillus thuringiensis, soil isolate, Vietnam, mosquitocidal activity, parasporin, cancer-cell-killing activity.

The anti-cancer effect of amygdalin on human cancer cell lines

2019 ◽  
Vol 46 (2) ◽  
pp. 2059-2066 ◽  
Author(s):  
Asghar Arshi ◽  
Sayed Mostafa Hosseini ◽  
Fataneh Saleh Khaje Hosseini ◽  
Zahra Yousefnejad Amiri ◽  
Fatemeh Sadat Hosseini ◽  
...  
Keyword(s):  
Cell Lines ◽  
Cancer Cell ◽  
Human Cancer ◽  
Cancer Cell Lines ◽  
Human Cancer Cell ◽  
Human Cancer Cell Lines ◽  
Anti Cancer

scholarly journals The role of membrane-bound metal ions in toxicity of a human cancer cell-active pore-forming toxin Cry41Aa from Bacillus thuringiensis

Toxicon ◽  
2019 ◽  
Vol 167 ◽  
pp. 123-133 ◽  
Author(s):  
Barbara Domanska ◽  
Eva Fortea ◽  
Michelle J. West ◽  
Jean-Louis Schwartz ◽  
Neil Crickmore
Keyword(s):  
Bacillus Thuringiensis ◽  
Metal Ions ◽  
Cancer Cell ◽  
Human Cancer ◽  
Human Cancer Cell ◽  
Membrane Bound

scholarly journals Characterization of 2,3,6,7,10,11-hexahydroxytriphenylene and its effects on cell viability in human cancer cell lines

2016 ◽  
Vol 94 (2) ◽  
pp. 205-211 ◽  
Author(s):  
Karine P. Naidek ◽  
Cristiane R. Zuconelli ◽  
Otavio M. Cruz ◽  
Ronny Ribeiro ◽  
Sheila M.B. Winnischofer ◽  
...  
Keyword(s):  
Cell Viability ◽  
Cell Lines ◽  
Cancer Cell ◽  
Human Cancer ◽  
Annexin V ◽  
Cancer Cell Lines ◽  
Human Cancer Cell ◽  
Paramagnetic Resonance ◽  
Human Cancer Cell Lines

We synthesized 2,3,6,7,10,11-hexahydroxytriphenylene (HHTP), characterized it by electrochemistry, spectroelectrochemistry, and electron paramagnetic resonance techniques, and evaluated its cytotoxicity to human cancer cell lines. The results revealed that HHTP has accessible higher-oxidation states, especially the tris-semiquinone monoradical. This species is stable and is formed after being stored for months. HHTP exhibited cytotoxic effects on 5 human cancer cell lines, including glioma and lung cancer cells. The cytotoxic effect was evaluated based on the decrease in cell viability, increases in the percentage of cells with fragmented DNA, and elevated numbers of annexin V–PI-positive cells after HHTP treatment.

scholarly journals Purification and Characterization of a Lectin from Arisaema tortuosum Schott Having in-vitro Anticancer Activity against Human Cancer Cell Lines

BMB Reports ◽  
2005 ◽  
Vol 38 (5) ◽  
pp. 526-532 ◽  
Author(s):  
Vikram Dhuna ◽  
Jagmohan Singh Bains ◽  
Sukhdev Singh Kamboj ◽  
Jatinder Singh ◽  
Shanmugavel ◽  
...  
Keyword(s):  
Anticancer Activity ◽  
Cell Lines ◽  
Cancer Cell ◽  
Human Cancer ◽  
Cancer Cell Lines ◽  
Human Cancer Cell ◽  
Purification And Characterization ◽  
Human Cancer Cell Lines

scholarly journals α-Tetralonyl Glucosides from the Green Walnut Husks of Juglans mandshurica and Their Antiproliferative Effects

Planta Medica ◽  
2019 ◽  
Vol 85 (04) ◽  
pp. 335-339
Author(s):  
An-dong Wang ◽  
Chao-jie Xie ◽  
Yun-qiang Zhang ◽  
Mei-chen Li ◽  
Xia Wang ◽  
...  
Keyword(s):  
Cell Lines ◽  
Cancer Cell ◽  
Human Cancer ◽  
2D Nmr ◽  
Cancer Cell Lines ◽  
Human Cancer Cell ◽  
Juglans Mandshurica ◽  
Antiproliferative Effects ◽  
Human Cancer Cell Lines

AbstractTwo new α-tetralonyl glucosides, (4S)-4,5,8-trihydroxy-α-tetralone-5-O-β-D-glucopyranosyl(1 → 6)-β-D-glucopyranoside (1) and (4S)-4,8-dihydroxy-α-tetralone-4-O-β-D-glucopyranosyl(1 → 6)-β-D-glucopyranoside (2), together with eight known compounds (3 – 10) were isolated from the green walnut husks of Juglans mandshurica. The structural characterization of all compounds was performed by spectroscopic analyses, including 1D and 2D NMR and HR-ESI-MS experiments. The isolated compounds were assayed for their cytotoxicity against two human cancer cell lines, A549 and HeLa. Four compounds (7 – 10) exhibited inhibitory effects against two human cancer cell lines with GI50 values between 1.3 and 5.8 µM.

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