Regulation of Nod factor sulphation genes in Rhizobium tropici CIAT899

2001 ◽  
Vol 47 (6) ◽  
pp. 574-579 ◽  
Author(s):  
Hamid Manyani ◽  
Carolina Sousa ◽  
María-Eugenia Soria Díaz ◽  
Antonio Gil-Serrano ◽  
Manuel Megías
Keyword(s):  
Wide Spectrum ◽  
Constitutive Expression ◽  
Upstream Region ◽  
Broad Host Range ◽  
Nod Factors ◽  
Rhizobium Tropici ◽  
Free Living ◽  
Nod Factor ◽  
Symbiotic Plasmid ◽  
Transcriptional Fusions

Rhizobium tropici CIAT899 is a tropical symbiont able to nodulate various legumes such as Leucaena, Phaseolus, and Macroptilium. Broad host range of this species is related to its Nod factors wide spectrum. R. tropici contains Nod factors sulphation nod genes, nodHPQ genes, which control nodulation efficiency in Leucaena. To study nodHPQ regulation, we carried out different interposon insertions in its upstream region. One of these generated interruptions, nodI mutant produced nonsulphated Nod factors suggesting a possible dependence of these genes on nodI upstream region. Moreover, analysis results of lacZ transcriptional fusions with these genes in symbiotic plasmid showed dependence of these genes on NodD protein. In order to determine nodHPQ organization, we studied the effect of interposon insertion upstream of each lacZ transcriptional fusion, and the data obtained was used to indicate that nodHPQ belong to the nodABCSUIJ operon. However, comparison between nodP::lacZ β-galactosidase activity in the symbiotic plasmid and in the pHM500 plasmid (containing nodHPQ genes) suggested constitutive expression in free living, and flavonoid inducible expression in symbiotic conditions. Constitutive nodHPQ expression may play a role in bacterial house-keeping metabolism. On the other hand, the transference of R. tropici nodHPQ genes to other rhizobia that do not present sulphated substitutions demonstrated that NodH protein sulphotransference is specific to C6 at the reducing end.Key words: Nod factors, nodHPQ genes, Rhizobium tropici, nod-box.

scholarly journals Genes Essential for Nod Factor Production and Nodulation Are Located on a Symbiotic Amplicon (AMPRtrCFN299pc60) in Rhizobium tropici

1998 ◽  
Vol 180 (11) ◽  
pp. 2866-2874 ◽  
Author(s):  
Patrick Mavingui ◽  
Toon Laeremans ◽  
Margarita Flores ◽  
David Romero ◽  
Esperanza Martínez-Romero ◽  
...  
Keyword(s):  
Insertion Sequence ◽  
Gene Dosage ◽  
Kanamycin Resistance ◽  
Nod Factors ◽  
Rhizobium Tropici ◽  
Resistance Marker ◽  
Nod Factor ◽  
Origins Of Replication ◽  
Symbiotic Plasmid

ABSTRACT Amplifiable DNA regions (amplicons) have been identified in the genome of Rhizobium etli. Here we report the isolation and molecular characterization of a symbiotic amplicon of Rhizobium tropici. To search for symbiotic amplicons, a cartridge containing a kanamycin resistance marker that responds to gene dosage and conditional origins of replication and transfer was inserted in the nodulation region of the symbiotic plasmid (pSym) of R. tropici CFN299. Derivatives harboring amplifications were selected by increasing the concentration of kanamycin in the cell culture. The amplified DNA region was mobilized into Escherichia coli and then into Agrobacterium tumefaciens. The 60-kb symbiotic amplicon, which we termed AMPRtrCFN299pc60, contains several nodulation and nitrogen fixation genes and is flanked by a novel insertion sequence ISRtr1. Amplification of AMPRtrCFN299pc60 through homologous recombination between ISRtr1 repeats increased the amount of Nod factors. Strikingly, the conjugal transfer of the amplicon into a plasmidlessA. tumefaciens strain confers on the transconjugant the ability to produce R. tropici Nod factors and to nodulatePhaseolus vulgaris, indicating that R. tropicigenes essential for the nodulation process are confined to an ampliable DNA region of the pSym.

scholarly journals Phaseolus vulgaris Recognizes Azorhizobium caulinodans Nod Factors with a Variety of Chemical Substituents

1999 ◽  
Vol 12 (9) ◽  
pp. 820-824 ◽  
Author(s):  
T. Laeremans ◽  
C. Snoeck ◽  
J. Mariën ◽  
C. Verreth ◽  
E. Martínez-Romero ◽  
...  
Keyword(s):  
Phaseolus Vulgaris ◽  
Host Plant ◽  
Wide Spectrum ◽  
Nod Factors ◽  
Azorhizobium Caulinodans ◽  
Rhizobium Tropici ◽  
Nod Factor ◽  
Mutant Strains

Phaseolus vulgaris is a promiscuous host plant that can be nodulated by many different rhizobia representing a wide spectrum of Nod factors. In this study, we introduced the Rhizobium tropici CFN299 Nod factor sulfation genes nodHPQ into Azorhizobium caulinodans. The A. caulinodans transconjugants produce Nod factors that are mostly if not all sulfated and often with an arabinosyl residue as the reducing end glycosylation. Using A. caulinodans mutant strains, affected in reducing end decorations, and their respective transconjugants in a bean nodulation assay, we demonstrated that bean nodule induction efficiency, in decreasing order, is modulated by the Nod factor reducing end decorations fucose, arabinose or sulfate, and hydrogen.

scholarly journals Sulfation of Nod Factors via nodHPQ Is nodD Independent in Rhizobium tropici CIAT899

1998 ◽  
Vol 11 (10) ◽  
pp. 979-987 ◽  
Author(s):  
Jorge Luis Folch-Mallol ◽  
Hamid Manyani ◽  
Silvia Marroquí ◽  
Carolina Sousa ◽  
Carmen Vargas ◽  
...  
Keyword(s):  
Phaseolus Vulgaris ◽  
Leucaena Leucocephala ◽  
Regulatory Region ◽  
Constitutive Expression ◽  
Nod Factors ◽  
Rhizobium Tropici ◽  
Independent Manner ◽  
Macroptilium Atropurpureum ◽  
Tlc Analysis ◽  
Layer Chromatography

A cosmid from the Rhizobium tropici CIAT899 symbiotic plasmid, containing most of the nodulation genes described in this strain, has been isolated. Although this cosmid does not carry a nodD gene, it confers ability to heterologous Rhizobium spp. to nodulate R. tropici hosts (Phaseolus vulgaris, Macroptilium atropurpureum, and Leucaena leucocephala). The observed phenotype is due to constitutive expression of the nodABCSUIJ operon, which has lost its regulatory region and is expressed from a promoter present in the cloning vector. Thin-layer chromatography (TLC) analysis of the Nod factors produced by this construction shows that it is still capable of synthesizing sulfated compounds, suggesting that the nodHPQ genes are organized as an operon that is transcribed in a nodD-independent manner and is not regulated by flavonoids. Se ha aislado un cósmido del plásmido simbiótico de Rhizobium tropici CIAT899 que contiene la mayoría de los genes de nodulación descrito para esta estirpe, menos el gen regulador nodD. La introducción de este cósmido en una estirpe curada del plásmido simbiótico de R. tropici CIAT899 permite la nodulación en las plantas ensayadas (Phaseolus vulgaris, Macroptilium atropurpureum, y Leucaena leucocephala). El fenotipo observado se debe a la expresión constitutiva del operón nodABCSUIJ bajo el promotor del gen de resistencia a la kanamicina, que lleva el vector donde se ha clonado el fragmento de ADN. Análisis por cromatografia de capa fina demuestran que esta construcción es capaz de sulfatar el extremo reductor del factor Nod. Estas evidencias sugieren que los genes nodHPQ constituyen un operón, y que su expresión es independiente del gen regulador nodD.

scholarly journals Sinorhizobium fredii HH103 Invades Lotus burttii by Crack Entry in a Nod Factor–and Surface Polysaccharide–Dependent Manner

2016 ◽  
Vol 29 (12) ◽  
pp. 925-937 ◽  
Author(s):  
Sebastián Acosta-Jurado ◽  
Dulce-Nombre Rodríguez-Navarro ◽  
Yasuyuki Kawaharada ◽  
Juan Fernández Perea ◽  
Antonio Gil-Serrano ◽  
...  
Keyword(s):  
Root Hairs ◽  
Broad Host Range ◽  
Capsular Polysaccharides ◽  
Dependent Manner ◽  
Nod Factors ◽  
Sinorhizobium Fredii ◽  
Nod Factor ◽  
Mesorhizobium Loti ◽  
Infection Threads ◽  
Surface Polysaccharide

Sinorhizobium fredii HH103-Rifr, a broad host range rhizobial strain, induces nitrogen-fixing nodules in Lotus burttii but ineffective nodules in L. japonicus. Confocal microscopy studies showed that Mesorhizobium loti MAFF303099 and S. fredii HH103-Rifr invade L. burttii roots through infection threads or epidermal cracks, respectively. Infection threads in root hairs were not observed in L. burttii plants inoculated with S. fredii HH103-Rifr. A S. fredii HH103-Rifr nodA mutant failed to nodulate L. burttii, demonstrating that Nod factors are strictly necessary for this crack-entry mode, and a noeL mutant was also severely impaired in L. burttii nodulation, indicating that the presence of fucosyl residues in the Nod factor is symbiotically relevant. However, significant symbiotic impacts due to the absence of methylation or to acetylation of the fucosyl residue were not detected. In contrast S. fredii HH103-Rifr mutants showing lipopolysaccharide alterations had reduced symbiotic capacity, while mutants affected in production of either exopolysaccharides, capsular polysaccharides, or both were not impaired in nodulation. Mutants unable to produce cyclic glucans and purine or pyrimidine auxotrophic mutants formed ineffective nodules with L. burttii. Flagellin-dependent bacterial mobility was not required for crack infection, since HH103-Rifr fla mutants nodulated L. burttii. None of the S. fredii HH103-Rifr surface-polysaccharide mutants gained effective nodulation with L. japonicus.

scholarly journals Rhizobium sp. Strain NGR234 and R. fredii USDA257 Share Exceptionally Broad, Nested Host Ranges

1999 ◽  
Vol 12 (4) ◽  
pp. 293-318 ◽  
Author(s):  
Steven G. Pueppke ◽  
William J. Broughton
Keyword(s):  
Glycine Max ◽  
Papua New Guinea ◽  
Broad Host Range ◽  
Nod Factors ◽  
Chamaecrista Fasciculata ◽  
Nod Factor ◽  
Australian Species ◽  
Apios Americana ◽  
Preferred Hosts ◽  
Rhizobium Sp

Genetically, Rhizobium sp. strain NGR234 and R. fredii USDA257 are closely related. Small differences in their nodulation genes result in NGR234 secreting larger amounts of more diverse lipo-oligosaccharidic Nod factors than USDA257. What effects these differences have on nodulation were analyzed by inoculating 452 species of legumes, representing all three subfamilies of the Leguminosae, as well as the nonlegume Parasponia andersonii, with both strains. The two bacteria nodulated P. andersonii, induced ineffective outgrowths on Delonix regia, and nodulated Chamaecrista fasciculata, a member of the only nodulating genus of the Caesalpinieae tested. Both strains nodulated a range of mimosoid legumes, especially the Australian species of Acacia, and the tribe Ingeae. Highest compatibilities were found with the papilionoid tribes Phaseoleae and Desmodieae. On Vigna spp. (Phaseoleae), both bacteria formed more effective symbioses than rhizobia of the “cowpea” (V. unguiculata) miscellany. USDA257 nodulated an exact subset (79 genera) of the NGR234 hosts (112 genera). If only one of the bacteria formed effective, nitrogen-fixing nodules it was usually NGR234. The only exceptions were with Apios americana, Glycine max, and G. soja. Few correlations can be drawn between Nod-factor substituents and the ability to nodulate specific legumes. Relationships between the ability to nodulate and the origin of the host were not apparent. As both P. andersonii and NGR234 originate from Indonesia/Malaysia/Papua New Guinea, and NGR234's preferred hosts (Desmodiinae/ Phaseoleae) are largely Asian, we suggest that broad host range originated in Southeast Asia and spread outward.

scholarly journals Regulation of hsnT, nodF and nodE genes in Rhizobium tropici CIAT 899 and their roles in the synthesis of Nod factors and in the symbiosis

Microbiology ◽  
2019 ◽  
Vol 165 (9) ◽  
pp. 990-1000
Author(s):  
Douglas Fabiano Gomes ◽  
Leandro Datola Tullio ◽  
Pablo del Cerro ◽  
Andre Shigueyoshi Nakatani ◽  
Amanda Alves Paiva Rolla-Santos ◽  
...  
Keyword(s):  
Nod Factors ◽  
Rhizobium Tropici

scholarly journals Rhizobial NodL O-Acetyl Transferase and NodS N-Methyl Transferase Functionally Interfere in Production of Modified Nod Factors

2001 ◽  
Vol 183 (11) ◽  
pp. 3408-3416 ◽  
Author(s):  
Isabel M. López-Lara ◽  
Dimitris Kafetzopoulos ◽  
Herman P. Spaink ◽  
Jane E. Thomas-Oates
Keyword(s):  
Nodule Formation ◽  
Signal Molecules ◽  
Nod Factors ◽  
Rhizobium Tropici ◽  
Methyl Transferase ◽  
Mesorhizobium Loti ◽  
Methyl Group ◽  
Rhizobial Species ◽  
Acetyl Group

ABSTRACT The products of the rhizobial nodulation genes are involved in the biosynthesis of lipochitin oligosaccharides (LCOs), which are host-specific signal molecules required for nodule formation. The presence of an O-acetyl group on C-6 of the nonreducingN-acetylglucosamine residue of LCOs is due to the enzymatic activity of NodL. Here we show that transfer of the nodLgene into four rhizobial species that all normally produce LCOs that are not modified on C-6 of the nonreducing terminal residue results in production of LCOs, the majority of which have an acetyl residue substituted on C-6. Surprisingly, in transconjugant strains ofMesorhizobium loti, Rhizobium etli, and Rhizobium tropici carrying nodL, such acetylation of LCOs prevents the endogenous nodS-dependent transfer of theN-methyl group that is found as a substituent of the acylated nitrogen atom. To study this interference betweennodL and nodS, we have cloned thenodS gene of M. loti and used its product in in vitro experiments in combination with purified NodL protein. It has previously been shown that a chitooligosaccharide N deacetylated on the nonreducing terminus (the so-called NodBC metabolite) is the preferred substrate for NodS as well as for NodL. Here we show that the NodBC metabolite, acetylated by NodL, is not used by the NodS protein as a substrate while the NodL protein can acetylate the NodBC metabolite that has been methylated by NodS.

scholarly journals The Sinorhizobium fredii HH103 MucR1 Global Regulator Is Connected With the nod Regulon and Is Required for Efficient Symbiosis With Lotus burttii and Glycine max cv. Williams

2016 ◽  
Vol 29 (9) ◽  
pp. 700-712 ◽  
Author(s):  
Sebastián Acosta-Jurado ◽  
Cynthia Alias-Villegas ◽  
Pilar Navarro-Gómez ◽  
Susanne Zehner ◽  
Piedad del Socorro Murdoch ◽  
...  
Keyword(s):  
Glycine Max ◽  
Cell Aggregation ◽  
Broad Host Range ◽  
Rna Seq ◽  
Free Living ◽  
Sinorhizobium Fredii ◽  
Global Regulators ◽  
Enhanced Production ◽  
Free Living Conditions

Sinorhizobium fredii HH103 is a rhizobial strain showing a broad host range of nodulation. In addition to the induction of bacterial nodulation genes, transition from a free-living to a symbiotic state requires complex genetic expression changes with the participation of global regulators. We have analyzed the role of the zinc-finger transcriptional regulator MucR1 from S. fredii HH103 under both free-living conditions and symbiosis with two HH103 host plants, Glycine max and Lotus burttii. Inactivation of HH103 mucR1 led to a severe decrease in exopolysaccharide (EPS) biosynthesis but enhanced production of external cyclic glucans (CG). This mutant also showed increased cell aggregation capacity as well as a drastic reduction in nitrogen-fixation capacity with G. max and L. burttii. However, in these two legumes, the number of nodules induced by the mucR1 mutant was significantly increased and decreased, respectively, with respect to the wild-type strain, indicating that MucR1 can differently affect nodulation depending on the host plant. RNA-Seq analysis carried out in the absence and the presence of flavonoids showed that MucR1 controls the expression of hundreds of genes (including some related to EPS production and CG transport), some of them being related to the nod regulon.

Plasmid replicons of Rhizobium

2005 ◽  
Vol 33 (1) ◽  
pp. 157-158 ◽  
Author(s):  
L.C. Crossman
Keyword(s):  
Short Review ◽  
Nitrogen Fixing ◽  
Rhizobium Etli ◽  
Free Living ◽  
Leguminous Plants ◽  
Symbiotic Plasmid ◽  
Rhizobium Spp ◽  
Plasmid Replicons ◽  
Rhizobium Sp

Rhizobium spp. are found in soil. They are both free-living and found symbiotically associated with the nodules of leguminous plants. Traditionally, studies have focused on the association of these organisms with plants in nitrogen-fixing nodules, since this is regarded as the most important role of these bacteria in the environment. Rhizobium sp. are known to possess several replicons. Some, like the Rhizobium etli symbiotic plasmid p42d and the plasmid pNGR234b of Rhizobium NGR234, have been sequenced and characterized. The plasmids from these organisms are the focus of this short review.

scholarly journals Draft Genome Sequence of Rhizobium tropici SARCC-755, a Free-Living Rhizobium That Nodulated and Promoted Growth in Pigeonpea [Cajanus cajan (L.) Millsp.]

2020 ◽  
Vol 9 (2) ◽  
Author(s):  
Francina Lebogang Bopape ◽  
Ahmed Idris Hassen ◽  
Zacharias H. Swanevelder ◽  
Eastonce T. Gwata
Keyword(s):  
Genome Sequence ◽  
Cajanus Cajan ◽  
Draft Genome ◽  
Soil Bacterium ◽  
Draft Genome Sequence ◽  
Rhizobium Tropici ◽  
Free Living ◽  
Content Type ◽  
Legume Plant ◽  
The Common

Rhizobium tropici SARCC-755 is a free-living soil bacterium that formed nodules on pigeonpea roots in the present study. However, the draft genome sequence reveals that this Rhizobium species contains the nolR gene but lacks the common nodulation (nodABC) genes and probably uses other pathways to induce nodules on the legume plant.

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