Isolation and characterization of ZH14 with antiviral activity againstTobacco mosaic virus

2008 ◽  
Vol 54 (6) ◽  
pp. 441-449 ◽  
Author(s):  
Wen-Wen Zhou ◽  
Li-Xiang Zhang ◽  
Bin Zhang ◽  
Fei Wang ◽  
Zhi-Hong Liang ◽  
...  
Keyword(s):  
Antiviral Activity ◽  
Mosaic Virus ◽  
Culture Filtrate ◽  
Plant Diseases ◽  
16S Rdna Sequence ◽  
Virus Inhibition ◽  
Isolation And Characterization ◽  
Phenotypic Tests ◽  
Number Of Bacteria

A large number of bacteria were isolated from plant samples and screened for antiviral activity against the Tobacco mosaic virus (TMV). The bacterium ZH14, which was isolated from Chinese Anxi oolong tea, secreted the antiviral substances, having 94.2% virus inhibition when the bacterial culture filtrate and TMV extract were mixed at a ratio of 1:1. The ZH14 strain is a gram-positive, spore-forming rod and has the ability to degrade ribonucleic acid. Based on its effectiveness on virus inhibition, ZH14 was selected for characterization and was identified as a strain of the Bacillus cereus group based on phenotypic tests and comparative analysis of its 16S rDNA sequence. At the same time, we determined the antiviral product of ZH14 as an extracellular protein with high molecular mass, having an optimum temperature of 15–60 °C and an optimum pH of 6–10. Hence, the ZH14 strain and its culture filtrate have potential application in controlling plant diseases caused by TMV.

Isolation and Characterization of Cell Lines Resistant to Crude Culture Filtrate of Fusarium in Chickpea

1994 ◽  
Vol 3 (1) ◽  
pp. 63-65 ◽  
Author(s):  
Surya Parkash ◽  
Anuradha Sehgal ◽  
Randhir Singh ◽  
J. B. Chowdhury
Keyword(s):  
Cell Lines ◽  
Culture Filtrate ◽  
Isolation And Characterization

Isolation and Characterization of a Phosphate-Solubilizing Bacterium Pseudochrobactrum sp. PSB13 from Petroleum-Contaminated Drill Cuttings

2014 ◽  
Vol 955-959 ◽  
pp. 407-410
Author(s):  
Li Bin Zhao ◽  
Xin Xin Wang ◽  
Chen Li ◽  
Yu Chen ◽  
Wei An ◽  
...  
Keyword(s):  
Drilling Process ◽  
Phosphate Solubilizing Bacteria ◽  
Phenotypic Characteristics ◽  
Drill Cuttings ◽  
16S Rdna Sequence ◽  
Isolation And Characterization ◽  
Bacterium Strain ◽  
Phosphate Solubilizing ◽  
First Time

Phosphate-solubilizing bacteria were extensively studied in many environment. However, little is known about them in drill cuttings, as wastes from drilling process. A phosphate-solubilizing bacterium strain PSB13 was isolated from petroleum-contaminated drill cuttings. This strain was identified asPseudochrobactrumsp. based on its 16S rDNA sequence and phenotypic characteristics. This strain could solubilize 97.6 μg/ml phosphates in 6 days when grown in NBRIP liquid medium. The increase in solubilization of phosphate coincided with the drop in pH, which indicates organic acid was responsible for the phosphate-solubilization. Phosphate-solubilizing bacterium was reported in drill cuttings for the first time, which suggests its potential in the bioremediation of petroleum-contaminated drill cuttings.

scholarly journals Detection, isolation, and characterization of the cucumber mosaic virus in Pseudostellaria heterophylla from Korea

2020 ◽  
Vol 47 (2) ◽  
pp. 150-156
Author(s):  
Da Hyun Lee ◽  
Jinki Kim ◽  
Jun Soo Han ◽  
Jae-Hyeon Lee ◽  
ByulHaNa Lee ◽  
...  
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Isolation And Characterization ◽  
Pseudostellaria Heterophylla

scholarly journals Isolation and characterization of Bacillus endophytic strains producers for non ribosomal lipopeptides NRLPs from tomato

2018 ◽  
Vol 9 (1) ◽  
pp. 128
Author(s):  
Walaa Hussein ◽  
Ramadan WA ◽  
Sameh Fahim
Keyword(s):  
High Performance ◽  
Plant Defence ◽  
Plant Diseases ◽  
Bacillus Species ◽  
Vegetable Crops ◽  
Defence Mechanisms ◽  
Isolation And Characterization ◽  
Genes Encoding ◽  
Plant Defence Mechanisms

Tomato (Solanum lycopersicum) are consid­ered one of the most important vegetable crops and infected by numbers of different diseases. Studying the use of biological alternatives, instead of chemical substances against plant diseases became necessary for the treatment by beneficial microorganisms endophytes, which can excrete natural products benefits to plant in reducing disease severity, promoting growth and inducing plant defence mechanisms. In this work, three endophytes strains were isolated from tomato stems and their 16srDNA have been found to belong to Bacillus species. The first strain was named BMG100, the second BMG101 and the third BMG102. Two Bacillus strains BMG100 and BMG101 have been found to harbour synthetases genes from three lipopeptides families; surfactin, plipastatin, and iturin (mycosubtilin) which can be detected by degenerated primers designed to detect the presence of synthetases genes encoding lipopeptides. The lipopeptides production was proved by their quantification using High Performance Liquid Chromatography (HPLC), whereas BMG100 produced 105, 178 and 293 mg/L of plipastatin, mycosubtilin and surfactin, respectively, BMG101 produced 385 mg/L of surfactin and 236 mg/L of mycosubtilin, while BMG102 showed no lipopeptides production. Keywords: Tomato; Endophytic bacteria; Lipopeptides; Bacillus species

scholarly journals Bacillus velezensis PEA1 Inhibits Fusarium oxysporum Growth and Induces Systemic Resistance to Cucumber Mosaic Virus

Agronomy ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 1312 ◽  
Author(s):  
Ahmed Abdelkhalek ◽  
Said I. Behiry ◽  
Abdulaziz A. Al-Askar
Keyword(s):  
Antiviral Activity ◽  
Fusarium Oxysporum ◽  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Plant Pathogens ◽  
Plant Diseases ◽  
Mass Spectrometry Analysis ◽  
Systemic Resistance ◽  
Bacillus Velezensis ◽  
Antiviral Activities

Bacillus velezensis manifests robust biocontrol activity against fungal plant pathogens; however, its antiviral activity has rarely been investigated. Bacillus velezensis strain PEA1 was isolated, characterized, and evaluated for antifungal and antiviral activities against Fusarium oxysporum MT270445 and cucumber mosaic virus (CMV) MN594112. Our findings proved that strain PEA1 had intense antagonist activity against F.oxysporum. Under greenhouse conditions, the antiviral activities (protective, curative, and inactivation) of PEA1-culture filtrate (CF) on Datura stramonium plants were assayed, using a half-leaf method. The inactivation treatment exhibited the highest inhibition rate (97.56%) and the most considerable reduction of CMV-CP accumulation levels (2.1-fold) in PEA1-CF-treated plants when compared with untreated plants (26.9-fold). Furthermore, PEA1-CF induced systemic resistance with significantly elevated transcriptional levels of PAL, CHS, HQT, PR-1, and POD genes in D. stramonium leaves after all treatments. Gas chromatography‒mass spectrometry analysis showed that pyrrolo[1,2-a]pyrazine-1,4-dione is the main compound in the PEA1-CF ethyl acetate extract, which may act as an elicitor molecule that induces plant systemic resistance and inhibits both fungal growth and viral replication. Consequently, B. velezensis can be considered as a potential source for the production of bioactive compounds for the management of plant diseases. To our knowledge, this is the first report of the antiviral activity of B. velezensis against plant viral infection.

scholarly journals Isolation and Characterization of a 66-kDa Antigen from Culture Filtrate of Aspergillus fumigatus NCPF2109.

1995 ◽  
Vol 36 (1) ◽  
pp. 25-32
Author(s):  
Jotika Boon-Long ◽  
Takeshi Mikami ◽  
Tomonori Murakami ◽  
Tatsuji Matsumoto ◽  
Minehiro Tojo ◽  
...  
Keyword(s):  
Aspergillus Fumigatus ◽  
Culture Filtrate ◽  
Isolation And Characterization
Sign in / Sign up

Export Citation Format

Share Document