scholarly journals Lignolytic Enzymes Production from Selected Mushrooms

2015 ◽  
Vol 3 (2) ◽  
pp. 308-313 ◽  
Author(s):  
H.M. Shantaveera Swamy ◽  
Ramalingappa
Keyword(s):  
Lignin Peroxidase ◽  
Manganese Peroxidase ◽  
Western Ghats ◽  
Agar Plate ◽  
Ligninolytic Enzymes ◽  
Enzyme Assays ◽  
Lignolytic Enzymes ◽  
Plate Assay

In this paper, ligninase enzymes produced by selected mushrooms have been reported. We collected mushrooms from Western Ghats, most of them were edible food. Thirty samples isolated were tested using a plate assay through direct agar plate assay by using ABTS, decolourisation containing the fifteen isolates were able to decolourise the dye, indicating a lignin-degrading ability. Spectrophotometric enzyme assays from all selected isolates were carried out to examine the production of Ligninolytic enzymes (Laccase, lignin peroxidase and manganese peroxidase). Ten selected isolates produced all three kinds of enzymes tested. Lignolytic enzymes are groups of enzymes these are actively involved in bioremediation.Int J Appl Sci Biotechnol, Vol 3(2): 308-313 DOI: http://dx.doi.org/10.3126/ijasbt.v3i2.12732 

scholarly journals Quantitative Evaluation of the Production of Ligninolytic Enzymes- Lignin Peroxidase and Manganese Peroxidase by P. Sajor Caju During Coir Pith Composting

CORD ◽  
2012 ◽  
Vol 28 (1) ◽  
pp. 10 ◽  
Author(s):  
Radhakrishnan S
Keyword(s):  
Lignin Peroxidase ◽  
Manganese Peroxidase ◽  
Tamil Nadu ◽  
Lignin Content ◽  
Ligninolytic Enzymes ◽  
Oxidative Enzymes ◽  
Aquatic Life ◽  
Coir Pith ◽  
Coir Fibre ◽  
Pleurotus Sajor Caju

Coir is the natural hard fruit fibre extracted from the exocarp of the coconut. The fibre has over 40 percent lignin and is spun into yarn and rope. Coir is used globally for manufacturing floor coverings as home furnishing. The Coir Industry enjoys the status as the largest cottage industry in Kerala giving employment to over a million people, of which 80 percent constitute women. Coir pith is a biomass residue generated during the extraction of coir fibre from coconut husk. Coir pith produced during coir fibre extraction is of environmental concern as its dumping on shore line and leaching of its constituents alter water quality and aquatic life. Management of coir pith is a major problem with all coir industrialists. Hillocks of coir pith accumulate in the vicinities of coir fibre extraction units in Kerala, Tamil Nadu, Andhra Pradesh, Karnataka, and Orissa. These agricultural wastes have traditionally been disposed by burning which resulted in various environmental problems. Therefore, composting is an alternate way to dispose coir pith and is of critical importance. Ligninolytic enzyme production during coir pith composting by Pleurotus sajor caju has been studied in detail. Pleurotus sajor caju produces oxidative enzymes which degrade lignin in the presence of urea as nitrogen source. Substitution of urea with vegetative sources has resulted in the vigorous growth of the mushroom which leads to decreased lignin content and C: N ratio in the biodegraded coir pith. Combination of Azolla and Soya hulls as biological supplements was observed to be the best substitute for lignin peroxidase and manganese peroxidase production. Activity of manganese peroxidase and lignin peroxidase was maximum on the twentieth day of fermentation of coir pith. The level of enzyme activity during biological composting using vegetative sources was compared with the conventional process using urea. The enzyme profile exhibited variation with change in substrate and duration of decomposition. The colonization of Pleurotus sajor caju by its utilization leads to biochemical changes in coir pith converting it into an ideal plant nutrient.

scholarly journals The Influence of Inducers on the Coltricia cinnamomea Laccase Activity and its Ability to Degrade POME

2021 ◽  
Vol 13 (2) ◽  
pp. 243-249
Author(s):  
Yohanes Bernard Subowo ◽  
Arwan Sugiharto
Keyword(s):  
Palm Oil ◽  
Lignin Peroxidase ◽  
Manganese Peroxidase ◽  
Laccase Activity ◽  
Ligninolytic Enzymes ◽  
Veratryl Alcohol ◽  
White Rot ◽  
Growth Media ◽  
Palm Oil Mill ◽  
Low Activity

Some species of Basidiomycetes, specifically white rot groups, produce three ligninolytic enzymes, namely, Lignin Peroxidase (LiP), Manganese Peroxidase (MnP) and Laccase (Lac), which have low activity in degrading Palm Oil Mill Effluent (POME). The research objective was to obtain the data on the ability of the Coltricia cinnamomea to produce LiP, MnP, and Lac enzymes to degrade POME. This research also studied the effect of sucrose, alcohol, veratryl alcohol, CuSO4 and ZnSO4,as inducers. Isolates of Coltricia cinnamomea, which were stored in a PDA media at -20℃ were obtained from the Microbiology section of the Research Center for Biology (LIPI). Furthermore, the growth media used were DM, Bean sprout Extract (TE) and PDB. The result indicated that PDB is the most suitable growth media for the production of ligninolytic enzymes, because in this medium these enzymes showed the highest activity. It was also observed that sucrose increased the laccase activity by 40.80%. Furthermore, Coltricia cinnamomea was able to reduce the concentration of Poly R-478 by 60.74%, after the addition of ZnSO4. In addition, it degraded and decreased the color and COD of POME, by 72.63% and 91.19% respectively, after the addition of veratryl alcohol, and incubation for 10 days. Therefore, this fungus can be used to degrade POME in order to prevent environmental pollution. Coltricia cinnamomea has not been used for POME degradation. By using Coltricia cinnamomea, we  obtained new data regarding the activity of laccase and its ability to degrade POME. 

Comparative production of ligninolytic enzymes by Phanerochaete chrysosporium and Polyporus sanguineus

2009 ◽  
Vol 55 (12) ◽  
pp. 1397-1402 ◽  
Author(s):  
Paramjit Kaur Bajwa ◽  
Daljit Singh Arora
Keyword(s):  
Phanerochaete Chrysosporium ◽  
Lignin Peroxidase ◽  
Manganese Peroxidase ◽  
Ligninolytic Enzymes ◽  
Culture Conditions ◽  
Malt Extract ◽  
Mineral Salts ◽  
Wide Range ◽  
Extract Medium ◽  
Malt Extract Medium

The aim of the present study was to compare the effect of a wide range of culture conditions on production of ligninolytic enzymes by Polyporus sanguineus and Phanerochaete chrysosporium . Lignin peroxidase production by P. sanguineus was comparable with that of P. chrysosporium, although the culture conditions giving the highest yield varied greatly between the two fungi. Highest yield of manganese peroxidase by P. sanguineus obtained in 0.5% malt extract medium and peptone or malt extract supplemented mineral salts broth could not be surpassed by P. chrysosporium in any of the optimization experiments. In addition to lignin peroxidase and manganese peroxidase, P. sanguineus also produced laccase, which was best expressed in malt extract medium supplemented with sugarcane bagasse.

Effects of ratio of manganese peroxidase to lignin peroxidase on transfer of ligninolytic enzymes in different composting substrates

2012 ◽  
Vol 67 ◽  
pp. 132-139 ◽  
Author(s):  
Meihua Zhao ◽  
Zhuotong Zeng ◽  
Guangming Zeng ◽  
Danlian Huang ◽  
Chongling Feng ◽  
...  
Keyword(s):  
Lignin Peroxidase ◽  
Manganese Peroxidase ◽  
Ligninolytic Enzymes

scholarly journals Lignin-degrading activity and ligninolytic enzymes of different white-rot fungi: effects of manganese and malonate

1997 ◽  
Vol 75 (1) ◽  
pp. 61-71 ◽  
Author(s):  
Tamara Vares ◽  
Annele Hatakka
Keyword(s):  
Lignin Peroxidase ◽  
Manganese Peroxidase ◽  
White Rot Fungi ◽  
Ligninolytic Enzymes ◽  
Growth Conditions ◽  
Fungal Species ◽  
White Rot ◽  
Trametes Hirsuta ◽  
Air Atmosphere ◽  
Trametes Trogii

Ten species of white-rot fungi, mainly belonging to the family Polyporaceae (Basidiomycotina), were studied in terms of their ability to degrade14C-ring labelled synthetic lignin and secrete ligninolytic enzymes in liquid cultures under varying growth conditions. Lignin mineralization by the fungi in an air atmosphere did not exceed 14% within 29 days. Different responses to the elevated Mn2+concentration and the addition of a manganese chelator (sodium malonate) were observed among various fungal species. This could be related with the utilization of either lignin peroxidase (LiP) or manganese peroxidase (MnP) for lignin depolymerization, i.e., some fungi apparently had an LiP-dominating ligninolytic system and others an MnP-dominating ligninolytic system. The LiP isoforms were purified from Trametes gibbosa and Trametes trogii. Isoelectric focusing of purified ligninolytic enzymes revealed the expression of numerous MnP isoforms in Trametes gibbosa, Trametes hirsuta, Trametes trogii, and Abortiporus biennis grown under a high (50-fold) Mn2+level (120 μM) with the addition of the chelator. In addition, two to three laccase isoforms were detected. Key words: white-rot fungi, lignin degradation, lignin peroxidase, manganese peroxidase, manganese, malonate.

scholarly journals Simultaneous Determination of Manganese Peroxidase and Lignin Peroxidase by Capillary Electrophoresis Enzyme Assays

ACS Omega ◽  
2017 ◽  
Vol 2 (10) ◽  
pp. 7329-7333 ◽  
Author(s):  
Sumire Kudo ◽  
Airi Harada ◽  
Hiroe Kubota ◽  
Keiko Sasaki ◽  
Takashi Kaneta
Keyword(s):  
Capillary Electrophoresis ◽  
Simultaneous Determination ◽  
Lignin Peroxidase ◽  
Manganese Peroxidase ◽  
Enzyme Assays

scholarly journals Degradation of lignosulfonic and tannic acids by ligninolytic soil fungi cultivated under icroaerobic conditions

2010 ◽  
Vol 53 (3) ◽  
pp. 693-699 ◽  
Author(s):  
Isis Serrano Silva ◽  
Cristiano Ragagnin de Menezes ◽  
Elisangela Franciscon ◽  
Eder da Costa dos Santos ◽  
Lucia Regina Durrant
Keyword(s):  
Lignin Peroxidase ◽  
Manganese Peroxidase ◽  
Soil Fungi ◽  
Carbon Sources ◽  
Ligninolytic Enzymes ◽  
Reversed Phase ◽  
Reversed Phase Hplc ◽  
Low Oxygen ◽  
Oxygen Conditions ◽  
Related Compounds

Soil fungi were evaluated regarding their ability to degrade lignin-related compounds by producing the ligninolytic enzymes. Lignosulfonic and tannic acids were used as sole carbon sources during 30 days under microaerobic and very-low-oxygen conditions. The fungi produced lignin-peroxidase, manganese-peroxidase and laccase . Expressive degradations was observed by C18 reversed-phase HPLC, indicating the biodegradation potential of these fungi, showing more advantages than obligate anaerobes to decontaminate the environment when present naturally.

scholarly journals Lignolytic Enzymes of a Mushroom Stereum ostrea Isolated from Wood Logs

2011 ◽  
Vol 2011 ◽  
pp. 1-6 ◽  
Author(s):  
K. Praveen ◽  
B. Viswanath ◽  
K. Y. Usha ◽  
H. Pallavi ◽  
G. Venkata Subba Reddy ◽  
...  
Keyword(s):  
Liquid Medium ◽  
Phanerochaete Chrysosporium ◽  
Lignin Peroxidase ◽  
Vegetative Growth ◽  
Manganese Peroxidase ◽  
Time Interval ◽  
Production Time ◽  
Lignolytic Enzymes ◽  
Culture Growth ◽  
Laccase Enzyme

Production of lignolytic enzymes by the mushroom fungus Stereum ostrea in liquid medium under conditions of vegetative growth was examined for 10 days in comparison to the reference culture Phanerochaete chrysosporium. Though growth and secretion of extracellular protein by S. ostrea were comparable to those of P. chrysosporium, yields of laccase enzyme by S. ostrea were higher than laccase titres of P. chrysosporium by more than 2 folds on the peak production time interval (IVth day of incubation). S. ostrea yielded titres of 25 units of laccase/ml as against 8.9 units of laccase/ml on the IVth day of incubation. Stereum ostrea also exhibited activities of other lignolytic enzymes, lignin peroxidase (LiP) and manganese peroxidase (MnP), higher than the reference culture. Growth of S. ostrea on the medium in the presence of Remazol orange 16 resulted in the decolourisation of dye, confirming the presence of lignolytic enzymes. S. ostrea appears to be a promising culture with complete lignolytic system.

scholarly journals Overview of Mycelial Fungi - Lignin Destructors

2022 ◽  
Author(s):  
Ivanova Lyudmila Afanasevna ◽  
FomenkoIvan Andreevich ◽  
Churmasova Lyudmila Alekseevna ◽  
Kuzmicheva Tatyana Pavlovna
Keyword(s):  
Enzyme Activity ◽  
Lignin Peroxidase ◽  
Manganese Peroxidase ◽  
Alcohol Oxidase ◽  
Ligninolytic Enzymes ◽  
Mycelial Fungi

In this work, the following genuses of mycelial fungi, capable of producing ligninolytic enzymes of various actions, were considered:Penicillium, Aspergillus, Fusariumand Altermaria. Fungi of the genus Aspergilluswere capable of producing laccase, manganese peroxidase and lignin peroxidase in the medium. Penicillium mostly produced laccase. Fusariumproduced laccase, aryl alcohol oxidase, manganesedependent peroxidase, manganese-independent peroxidase and lignin peroxidase. Alternariaproduced laccase, lignin peroxidase and manganese peroxidase. The results demonstrated the possibility of using specific substrates in the study of enzyme activity, as well as the influence of some factors introduced into the medium on the synthesis of enzymes. The auxiliary influence of these fungi on the synthesis of ligninolytic enzymes in symbiosis with otherswas considered. Keywords: mycelial fungi, ligninolytic enzymes, Penicillium, Aspergillus, Fusarium, Altermaria

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