Lactobacillus casei as a probiotic in malnourished Giardia lamblia-infected mice: a biochemical and histopathological study

2011 ◽  
Vol 57 (2) ◽  
pp. 127-135 ◽  
Author(s):  
Geeta Shukla ◽  
Ramandeep Kaur Sidhu
Keyword(s):  
Epithelial Cells ◽  
Giardia Lamblia ◽  
Lactobacillus Casei ◽  
Membrane Integrity ◽  
Mucosal Damage ◽  
Oral Feeding ◽  
Daily Administration ◽  
Histopathological Study ◽  
Microvillus Membrane

The study describes the in vivo activity of Lactobacillus casei in malnourished Giardia lamblia -infected BALB/c mice. By experimentation, it was found that daily administration of the probiotic 7 days before inoculation with Giardia trophozoites in malnourished mice efficiently reduced both the severity and duration of giardiasis. More specifically, excretion of Giardia cysts and trophozoites counts were reduced, while faecal lactobacilli counts increased significantly in probiotic-fed malnourished mice, compared with control mice. Interestingly, it was also observed that oral feeding of the probiotic to malnourished mice abrogated all the anthropometric and biochemical anomalies. Histologically, morphological and cellular alteration of microvillus membrane integrity revealed that probiotic administration ameliorated the mucosal damage in malnourished, probiotic-inoculated, Giardia-infected mice compared with the severe microvillus atrophy, œdematous and vacuolated epithelial cells, and ileitis in malnourished Giardia-infected mice. The results clearly show the antigiardial effect of the probiotic in vivo by modulating the gut cells to inhibit the colonization and multiplication of Giardia trophozoites, thus reducing the severity and duration of murine giardiasis.

Protective potential of L. acidophilus in murine giardiasis

Open Medicine ◽  
2010 ◽  
Vol 5 (4) ◽  
pp. 456-463 ◽  
Author(s):  
Geeta Shukla ◽  
Tarveen Kaur ◽  
Rakesh Sehgal ◽  
Praveen Rishi ◽  
Vijay Prabha
Keyword(s):  
Histological Analysis ◽  
Villous Atrophy ◽  
Membrane Integrity ◽  
Mucosal Damage ◽  
Post Inoculation ◽  
Intestinal Epithelial ◽  
Giardia Infection ◽  
Probiotic Treatment ◽  
Igg Level

AbstractThis study describes the in vivo activity of Lactobacillus acidophilus in Giardia lamblia infected BALB/c mice. Experimentally, it was observed that daily administration of lactobacilli 7 days before or in simultaneous inoculation with Giardia trophozoites efficiently reduced G. lamblia infection in mice. More specifically, excretion of Giardia cysts were reduced significantly in probiotic-treated groups, and resolution of infection was observed by day 21 post-inoculation. It was also observed that the lactobacillus count increased tremendously and continuously in faeces of all probiotic-fed mice, and was significantly higher as compared with that in control mice. Histological analysis of microvilli membrane integrity revealed that probiotic administration also protected mice against parasite-induced mucosal damage, whereas Giardia-infected mice had severe villous atrophy, oedema, vacuolation and ileitis. Immunologically, the anti-Giardia serum IgG level was not stimulated significantly by probiotic treatment administered both prior to and simultaneous with Giardia infection, but remained high after the infection peak. Taken together, the data demonstrates the anti-giardial effect of the probiotic in vivo by modulation of the intestinal epithelial cells, inhibiting the colonization of Giardia trophozoites and thereby reducing the severity of Giardia infection.

Growth and characterization of porcine urinary bladder epithelial cells in vitro

1994 ◽  
Vol 266 (2) ◽  
pp. F298-F308 ◽  
Author(s):  
C. Guhe ◽  
W. Follmann
Keyword(s):  
Epithelial Cells ◽  
Urinary Bladder ◽  
Cultured Cells ◽  
Apical Cell ◽  
Membrane Integrity ◽  
Membrane Vesicles ◽  
Lactate Dehydrogenase Activity ◽  
And Function

Dividing long-term monolayer cultures of porcine urinary bladder epithelial cells were obtained by a combined mechanical and enzymatic isolation method. The serum-free cultured cells were investigated morphologically and characterized according to their growth characteristics and enzymatic functions. Investigations over a period up to 12 wk demonstrated that the cells regain their in vivo polarization with apically situated membrane vesicles and tight junctions between neighboring cells when they have built up a confluent monolayer. Activities of most marker enzymes for cell-differentiated status and function of such cells observed over a period of 4 wk in culture were conserved compared with the original tissue. Lactate dehydrogenase activity release into the medium was at low levels (< or = 5% of the total amount), indicating a good membrane integrity and cell viability. The chromosome set (2n = 38) did not change significantly during the first 5 wk, but, with additional culture time, the degree of polyploid and polynucleated cells increased comparable to the in vivo situation, in which the apical cell layer of the bladder mucosa also showed a high degree of polynucleation and polyploidy, indicative of a senescence process.

scholarly journals Lactobacillus johnsonii La1 Antagonizes Giardia intestinalis In Vivo

2005 ◽  
Vol 73 (2) ◽  
pp. 1265-1269 ◽  
Author(s):  
Martín A. Humen ◽  
Graciela L. De Antoni ◽  
Jalil Benyacoub ◽  
María E. Costas ◽  
Marta I. Cardozo ◽  
...  
Keyword(s):  
Cellular Response ◽  
Membrane Integrity ◽  
Treated Group ◽  
Mucosal Damage ◽  
Meriones Unguiculatus ◽  
Giardia Intestinalis ◽  
Lactobacillus Johnsonii ◽  
Enzymatic Markers ◽  
Probiotic Lactobacilli

ABSTRACT This study describes the in vivo activity of Lactobacillus johnsonii La1 (NCC533) in Giardia intestinalis-infected gerbils (Meriones unguiculatus). Daily administration of lactobacilli in the drinking water from 7 days before inoculation with Giardia trophozoites efficiently prevented G. intestinalis strain WB clone C6 from infecting gerbils. More specifically, shedding of fecal Giardia antigens (GSA65 protein) was diminished in the La1-treated group, and resolution of infection was observed by 21 days postinoculation. Histology and analysis of enzymatic markers of microvillus membrane integrity revealed that probiotic administration also protected against parasite-induced mucosal damage. In addition, a cellular response to Giardia antigens was stimulated in spleen cells from La1-treated gerbils. Results show for the first time the antigiardial effect of probiotic lactobacilli in vivo and provide further insight into the antagonistic properties of lactic acid bacteria against protozoa involved in intestinal infections.

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